Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"collagen hydrogels"”.
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The mechanics of cell-seeded collagen hydrogels
… permeability and stiffness, of HepG2 cell-seeded collagen hydrogels. Confined compression testing in conjunction with biphasic theory modelling was utilised to provide measures of these mechanical parameters of interest. Different cell seeding densities and culture durations were tested to observe …
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Development of a Workflow to study Neuronal Injury In Vivo in the Retina and In Vitro in Collagen Hydrogels
… neuroblastoma cell line into neurons in 3D collagen hydrogels. The protocol determined the optimal collagen concentration and seeding density. SH-SY5Y differentiation was effective in a 0.5 mg/mL and 1.0 mg/mL collagen hydrogels seeded at a density of 6x105 cells/mL. Next, a method to …
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Substrate elasticity regulates the biophysical properties of hematopoietic stem and progenitor cells
… femurs and tibias were cultured in or on top of collagen hydrogels or on top of 2D polyacrylamide (PA) gels with varying mechanics and ligand densities. With collagen hydrogels and type I collagen-coated PA gels with varying stiffness, simple in vitro biomaterials system to probe the effects of …
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Biomechanical biomarkers of cancer
… for determining the mechanical properties of collagen hydrogels. Therefore, FEBio was used to create both force and displacement control confined compression tests of tissues modelled by collagen hydrogels. The tissue models contained a 'tumour' layer and the effects of varying the stiffness …
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Mechanics and transport characterization of bioengineered tissue microenvironment platforms
… In this work, extensive characterization of collagen I hydrogels, popular for use as extracellular matrix mimics, was performed in order to enable tuning to specific in vivo conditions. Additionally, perfusion of blood in a 3D tissue microenvironment platform fabricated using collagen …
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Bacteria - Hydrogel Interactions: Mechanistic Insights via Microelastography and Deep Learning
… motile behavior within human tissue phantoms (collagen and agar) and the bacteria-hydrogel interactions. Apart from motility, it is important to analyze the mechanical properties of the hydrogels the bacteria interact with as they play a vital role in overall behavior and physics of bacteria …
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3-D Bio-inspired Microenvironments for In Vitro Cell Migration
… devices in PDMS to create 3-D structures in collagen hydrogels to mimic native tissue structures. We have studied the interaction of endothelial cells with model geometries of blood vessels in collagen hydrogel at different concentrations to mimic the biomechanical properties of tissues …
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Rational Engineering of Bacteria and Biohybrids for Enhanced Transport and Colonization in the Tumor Microenvironment
… of extracellular matrix (ECM) proteins such as collagen. Coupled with limited specificity, the high chemotherapeutic doses needed to effectively treat tumors often lead to unacceptable levels of damage to healthy tissues. Bacteria-based cancer therapy (BBCT) is an innovative alternative. …
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NOVEL NANOFIBER-BASED GRAFT FOR HEART VALVE REPLACEMENT
… tissue engineering. While processed conventional collagen-based heart valves have been used for many years, they eventually fail because of inadequate cell infiltration and insufficient replacement of graft material by tissue remodeling. To overcome the graft failure due to inadequate tissue …
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Scleraxis-mediated regulation of tendon and ligament cell mechanobiology
… combined with mechanical strain in a 3D collagen hydrogel model was investigated as a means of deriving mature ligament cells from stem cells for use in ligament tissue engineering. Scx overexpression in C3H10T1/2 cells cultured in collagen hydrogels under static strain resulted in …
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Engineering chemoattractant gradients using controlled release polysaccharide microspheres
… around single source cells. Further we used 3D collagen hydrogels embedded with ASCs as an in vitro model to investigate single human T-cell and dendritic cell migration dynamics to CCL21 and CCL19 chemokine gradients. Individual T-cells exhibited a binary response to isolated attractant …
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COLLAGEN AND ANTIOXIDANTS FROM SEA URCHINS: WASTE VALORIZATION CHALLENGES TOWARDS REGENERATIVE MEDICINE
… that sea urchin waste contains highly valuable collagen derived from by-product tissues such as the peristomal membrane, which can be used as an innovative biomaterial for tissue engineering. The remaining part of the waste (test and spines) can be an additional source of biologically …
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Keratose Hydrogels Promote Vascular Smooth Muscle Differentiation from c-kit+ Human Cardiac Stem Cells: Underlying Mechanism and Therapeutic Potential
… cardiac regeneration. My studies utilize KOS hydrogels to modulate c-kit+ hCSC differentiation, and explore the capability of differentiated cells to regenerate vascular tissue. In the first Chapter, we reviewed literature relevant to keratin-based biomaterials and their biomedical …