Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"collagen gel"”.
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Stimulation of angiogenesis through collagen gel by applying shear stress and interstitial flow
… this chamber allows the cells to be seeded on collagen gel rather than a rigid substrate to more closely mimic in vivo environment. A shear stress of I Pa was applied to the endothelial cells for a duration of 36 hours. Results show that after flow was initiated for this duration, a change in …
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Development of a collagen gel sandwich hepatocyte bioreactor for detecting hepatotoxicity of drugs and chemicals
… that culturing hepatocytes between two layers of collagen type I maintains in vivo-like morphology and also many drug metabolizing enzymes for weeks. In spite of the research examining drug metabolism in collagen sandwiches, there are very few studies evaluating this system for investigating …
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Studies on collagen stability and mechanical properties in tissue engineering applications
… This project aims to measure the stiffness of collagen based gel matrix. This work provides mechanical study of 0.3 % collagen hydrogel monolayer properties under compression. In order to understand how the collagen gel properties change in relation with time and cell concentrations, the …
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The Role of Mechanical Interactions between Cells and the Extracellular Matrix on Mineralization in a Stem Cell-Seeded Scaffold: a Multi-Scale Approach
… healing rate in difficult-to-heal cases. Cell-collagen gel constructs have been recently used in several studies to promote mineralization. However, the role of mechanical factors to accelerate fracture repair has not been optimized nor is fully understood. The goal of this study was to …
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Biomimetic and vascularized 3-d liver cancer model
… cells, are fabricated and encapsulated in collagen gel; 2) heparin crosslinked, wet-spun chitosan microfiber/electrospun chitosan mat tube are coated with endothelial cells; and 3) spheroids and endothelial coated electrospun chitosan mat tube are embedded together on Matrigel. Using the …
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Investigation of Neutrophil-Like HL-60 Cell Migration in a 3D Collagen Matrix
… cell migration patterns, we designed a 3D hydrogel model to create a concentration gradient inside a collagen gel that contained neutrophil-like (dHL-60) cells. Our experimental setup and computational analysis allowed us to study the cell migration indicators such as percentage and velocity of …
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Synthetic peptides derived from decorin as building blocks for biomaterials based on supramolecular interactions
… of the binding capability of the peptides to Collagen I was performed by surface plasmon resonance analyses, from which all but one of the peptides representing the inner surface of decorin showed binding affinity to collagen with values of dissociation constant between 2•10-7 M and 2.3•10-4 …
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Microvessel structure formation in a 3D perfused co-culture of rat hepatocytes and liver endothelial cells
… cells were not observed in parallel 2D collagen gel sandwich cultures. Immunohistochemistry further confirmed the presence of sinusoidal endothelia within the 3D co-culture tissue, as well as other non-parenchymal cells in both 3D mono-culture and co-culture.
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Evaluation of Prototype Cell Delivery Catheters Using Agarose Gel and Cell Culture Experiments
… 2.0 mm large bore) were evaluated using agarose gel and cell culture experiments. We initially delivered pheochromocytoma (PC 12) cells through a 25-gauge syringe needle to optimize cell growth. We observed in the agarose gel experiments that when the inner catheter was filled and then inserted …
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Stiffness of vascular smooth muscle cells from aged primates measured using reconstituted tissue model
… has implicated stiffening of the extracellular collagen matrix with age. The study reported in this thesis focused instead on the potential contribution from stiffening of the vascular smooth muscle cells (VSMCs) with age. VSMCs previously obtained from young and old monkeys (macaca …
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Characterisation of the expression profile and endothelial function of Rho GTPase RhoJ
… focal adhesions, actin stress fibres and collagen gel contraction, suggesting increased actomyosin contractility. Pharmacological inhibition of ROCK and myosin II, two regulators of actomyosin contractility, restored motility and tube formation after RhoJ knockdown. RhoJ localised to blood …
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Fabrication and evaluation of a collagen-based fiber-gel three-dimensional construct for peripheral nerve repair
… nervous tissue constructs consisting of type I collagen are fabricated into a composite biomaterial scaffold to promote contact-guided growth of neuronal and glial cultures in vitro. The growth of adult tissue on these collagen-based materials is further evaluated. These constructs are assembled …
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Quantitative modeling and control of nascent sprout geometry in in vitro Angiogenesis
… a new microfluidic assay consisting of a collagen gel scaffold with features to reduce assay-to-assay variability and increase experimental throughput. This high throughput assay reveals that there is an inverse relationship between nascent vessel elongation rate and diameter under diverse …
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Use of gene expression to characterize heterogeneous liver cell populations
… and kupffer cells (KC). The same analysis of 2D collagen gel sandwiches shows the presence of all NPC cell types. This indicates that our process is currently limited by the ability of EC's and KC's to incorporate into spheroid aggregates. Future work that validates the temporal expression …
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The extraction of type 1 collagen and the fabrication of multi-filament embedded hydrogels for guided nerve regeneration
… by using natural materials such as type I collagen, which is the largest constituent of the extra- cellular matrix of living tissue. In this project, fabrication of novel constructs for nerve tissue guidance was carried out using homogenous hydrogels and multi-filament arrays of wet-spun …
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Characterisation of Cutaneous Wound Healing Process in Naked Mole Rats
… of reduced scarring in NMR wounds including low collagen I to III ratio, increased HA expression (HMW) and increased fibronectin expression. Transcriptional profiling of TGFβ isoforms and different pro/anti-inflammatory cytokines revealed a balance in the expression and repression of different …
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Bioinspired soft matter self-assembly and its use in biomedical applications
… a dynamic collection of polymers, colloids, and gels. Therefore, most biological matter is soft matter, and many biomedical products, such as 3D cell culture platforms or nanocarriers for drug and imaging agent delivery, often consist mostly of soft matter. Despite advances in these fields, …
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Nanoparticle effects on cell migration
… (PDGF). Cells were cultured and placed into 3D collagen gel in a specialized chemotaxis slide, in which a steady chemoattractant concentration was produced. Cell migration was recorded through bright-field optical microscopy. Gold nanospheres with different surface chemistries were introduced …
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Design of a Gel-in-Scaffold Hybrid Substrate for a Multicellular Endometrial Model
… have been developed using scaffolds or hydrogels to study the interactions between the endometrium and blastocysts. However, these models fail to fully replicate cellular complexity, and lack key structural features vital for successful blastocyst implantation. This thesis explores a range of …
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Characterization of the cardiogenesis of embryonic stem cells
… systematically studied. Next, the effects of collagen I and cell alignment, two biophysical signatures of the adult myocardium, on promoting phenotypic changes of isolated embryonic stem cell derived cardiomyocytes (ESCDMs) were investigated. Effects of collagen I depended on how it was …
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