Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 3418 for “"coli"”.
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Genomic Approaches for the Characterization of Shiga-toxigenic Escherichia coli (E. coli)
… disability, and loss of life. Shiga-toxigenic E. coli (STEC) are a significant cause of foodborne illness in Canada, and consequently, it is of high priority to improve detection, isolation, and characterization methods to mitigate their impact on public health. While current methods generally …
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Calcium homeostasis in Escherichia coli
… role as overactive calcium efflux systems in E. coli. My data also raises the distinct possibility of calcium's involvement in stress response as evidenced by upregulation of marB in transposon mutants and by 10-fold induction of rpoS in elevated calcium condition in mutants defective in calcium …
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Superoxide Toxicity in Escherichia Coli
… copper is unavailable. Mutants of sodC in E. coli and S. typhimurium suffer damage during aerobic growth from a cellular source of superoxide. The unknown damage sensitizes the mutants to subsequent challenges with H2O2. This result indicates that there is a role for CuZnSOD other than …
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Superoxide stress in Escherichia coli
… were shown to be inactivated in the Escherichia coli strain which lacks the ability to scavenge O2- (denoted as SOD- mutant). Moreover, the inactivation of DAHP synthase, the first enzyme in the aromatic amino acid biosynthetic pathway, could partially explain the aromatic amino acid auxotrophy …
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Biotin synthesis in Escherichia coli
… acid. However, the mechanism whereby E. coli assembles this pimelate intermediate was unclear. Genetic analyses identified only two genes of unknown functions, bioC and bioH, which are required for pimelate synthesis. BioC is annotated as an S-adenosyl-L-methionine (SAM) dependent …
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Electrofusion of Escherichia coli with Giant Lipid Vesicles and the Compaction of Escherichia coli Nucleoid
… applied for inducing the fusion of Escherichia coli-derived biological vesicles and synthetic lipid vesicles and proved to have a great potential as a new tool for delivering bulky cargos into biological vesicles.
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Directed evolution of antimutator E. coli
… used to engineer antimutators in Escherichia coli, Periodic Reselection for Evolutionarily Reliable Variants (PResERV). In this first PResERV experiment, I observe that the antimutator phenotype is due to mutations in genes involved DNA replication and RNA metabolism (polA, polB, and rne). In …
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Tryptophanase Regulatory Mechanisms in Escherichia coli
… species, but most studied in *Escherichia coli*. Indole is formed by the degradation of L-tryptophan through the action of the enzyme tryptophanase (TnaA). The *tnaA* gene is part of the tryptophanase (*tna*) operon, which is regulated by catabolite repression and tryptophan-induced …
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Crystalline Cytochrome B1 From Escherichia Coli
Made available in DSpace on 2014-12-09T17:34:53Z (GMT). No. of bitstreams: 1 6408372.pdf: 4879635 bytes, checksum: 1adaafe1b90c03b17de7b22e97b56c0f (MD5) Previous issue date: 1964
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How Escherichia coli uses environmental cysteine
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01
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Shedding light on E. coli phototaxis
… has been focused on phototaxis response in E. coli. I will review the current state of knowledge on chemotaxis and phototaxis and discuss the motivation behind this project in Chapter 1. In Chapters 2 and 3, I will describe a high-throughput framework for studying phototaxis in bacteria, which …
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Rewriting the genome of Escherichia coli
… reported strategies for genome engineering in E. coli (REXER and GENESIS) are extended and used to create a synthetic, recoded E. coli genome. Chapter 2 describes a strategy for assembling large natural genomic DNA pieces into BACs that are substrates for genome replacement. Experiments with these …
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Algorithms for E. coli genome engineering
Author summary: Lamba red recombineering is one of methods of performing genome engineering. However, this method of genome editing is not very specific and efficient and is highly dependent on the genomic regions that are targeted (integration sites). In this project we explored ways of …
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Investigation of Escherichia coli [NiFe]-Hydrogenase Maturation
… to the [NiFe]-hydrogenase in Escherichia coli was examined using microbiological, biochemical, spectroscopic, and computational methods. The results demonstrate that protein-protein interactions between the metallochaperones afford layers of nickel selectivity. The protein complexes …
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Die Regulation der Transkriptionsantitermination in Escherichia Coli.
… bestehend aus den Escherichia coli (E. coli) Nus-Proteinen (A, B, E, G), der RNAP, der nut RNA-Sequenz und dem Lambda N-Protein. Die ribosomalen (rrn) Operons in E. coli werden durch intrinsische Antitermination reguliert. Fluoreszenz-Anisotropie-Messungen konnten zeigen, dass …
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