Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"cold shock"”.
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Cold Shock Domain Proteins in Normal and Leukemic Hematopoiesis
<p>Cold shock domain (CSD) proteins are the most evolutionarily conserved family of nucleic-acid binding proteins. There are four functional genes that contain CSDs in humans and mice: YBX1, MSY4, MSY2, and CSDE1. YBX1 is overexpressed in most cancers, and is frequently associated with poor …
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Characterisation of the cold-shock response in Mycobacterium smegmatis
The response of Mycobacterium smegmatis to a cold shock was investigated in order to gain insight into the stress responses of members of the genus Mycobacterium. Mycobacterium smegmatis cultures were shocked from 37°C to 30°C, 25°C, 15°C, and 10°C and the effects on both growth (ATP concentration, …
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The role of the cold shock protein, RBM3, in cooling, synaptic structural plasticity and neuroprotection
… regeneration, driven by failed induction of the cold shock protein, RNA-binding motif protein 3 (RBM3). Overexpression of neuronal RBM3, either by cooling or lentiviral delivery, restores synapse regeneration, preventing subsequent neuronal loss and significantly prolonging survival. How RBM3 …
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Control of Passive Influx of Calcium in Corn Root Tissue (calcium-2+ Channels, Ion Transport, Voltage-Regulator, Injury, Cold Shock)
… as in animal cells. Environment stresses or shocks such as low and high temperatures, wounding, low pH, or application of metabolic inhibitors which lead to depolarization of the cell potential, increase the passive entry of Ca('2+) to the cells. Voltage-control is lost upon severe injury, …
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I. Identification of thecis-regulatory elements of the Arabidopsis thaliana TCH3 gene. II. Developmental and molecular responses to touch stimulation in Arabidopsis mutants defective in ethylene responses
… of TCH3 following touch, darkness, heat shock, and cold shock, transgenic plants harboring fusion genes containing 5$\sp\prime$ fragments of the TCH3 locus fused to the reporter gene uidA were created. A 43 bp sequence adjacent and 5$\sp\prime$ to the transcription unit of TCH3 is …
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Molecular adaptation to low-temperature in New Zealand stick insects
… New Zealand suggests these species have suitable cold-tolerant phenotypes and genotypes warranting investigation. The objectives of this thesis were to: (i) verify the application of RNA-Seq to identify mild cold-shock responsive genes in an alpine stick insect (Micrarchus nov. sp. 2); (ii) …
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Stress-induzierte posttranslationale Modifikationen des Y-box Proteins-1 (YB-1) beinhalten Acetylierung und Phosphorylierung
YB-1 is a member of the cold-shock domain protein superfamily. It performs a wide variety of cellular functions, including transcriptional and translational regulation, DNA repair, drug resistence and stress responses to extracellular signals. We demonstate increasing levels of acetylated …
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Herstellung monoklonaler Antikörper gegen YB-1 und Charakterisierung ihrer Bindungseigenschaften
Y-box protein-1 (YB-1) belongs to the cold shock protein family. Members of this family are highly conserved in evolution and fulfill numerous functions in gene regulation and mRNA translation. Here, we have successfully generated six distinct anti-YB-1 monoclonal antibodies by immunizing mice with …
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Genetic interaction landscape of the Escherichia coli transcriptional factor machinery
… putrescine pathway and efflux pump, and cold shock adaptation, respectively. Pan-bacterial conservation suggests that TF genes with similar GI profiles are co-conserved in bacterial evolution. Moreover, the E. coli TF GI network provided deep insights on conserved genetic association …
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Nuclear Transfer With Porcine Embryonic Stem Cells
… ethanol had no effect on activation rates, (2) cold shock, sham enucleation, electroactivation and electroactivation + culture with cycloheximide improved activation rates, and (3) electroactivation + culture with cycloheximide was the most efficient treatment for production of pronuclear stage …
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Studies into Mg-preconditioning of brewer’s yeasts
… yeast cells was altered by temperature shock (both cold shock at 4°C and heat shock at 40°C) and toxic ethanol shock (at 10-20%). This resulted in significant cellular losses of Mg, which indicated that some of the extra Mg present in these cells was in a free (releasable) form rather …
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Stressed Induced Changes in <i>Karenia brevis</i> Ribosomal RNA
… “stress” conditions, namely nutritional stress, cold shock, and decreased salinity. When the total RNA is extracted we have detected aberrant rRNA bands on the microcapillary electrophoresis BioAnalyzer (Agilent, Inc.) Interestingly when stressed, the large ribosomal subunit (LSU) becomes larger …
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Transcriptomic analysis of the response of the lactic acid bacteria Lactococcus piscium for the identification of possible adaptive genes during thermal shock in cold and warm temperatures
… this study, to analyze in a transcriptome level, cold and heat shock stress to spoilage lactic acid bacteria, a time-dependent RNA-seq for Lactococcus piscium with a temperature of 0 °C and 28 °C was conducted. The data of protein regulation during the experiment shows that Lactococcus piscium has …
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Identifizierung und Charakterisierung von Genen für die Entwicklung des cerebralen Cortex
… besitzt mit der stark konservierten putativen "cold-shock"-Domäne die Fähigkeit, mit DNA und RNA zu interagieren und somit potentielle Transkriptionsfaktor-Aktivität.Zur Funktionsanalyse von Maus-PIPPin in der Corticogenese wurde das Gen kloniert und mittels homologer Rekombination inaktiviert. …
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Investigating temperature signalling pathways in Arabidopsis thaliana using small molecules
Upon exposure to heat or cold, Arabidopsis thaliana seedlings undergo rapid transcriptional reprogramming of several hundreds of genes that promote stress tolerance. Despite extensive characterisation of the transcriptional responses to these stimuli, however, relatively little is known about the …
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Mechanisms Underlying Variation in Insect Chill Tolerance: The Role of Ion and Water Transport
… temperatures; loss of this homeostasis in the cold likely contributes to chronic chilling injury, and reestablishment of homeostasis is required for recovery from chilling. Both plastic and interspecific variation in cold tolerance correlates with enhanced defense of water and ion homeostasis …
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Effects Of Cholesterol On Bovine Sperm Survival During Cooling and Freezing
… the sperm was examined prior to freezing, after cold shock treatment, 0 and 3 hrs after cooling, and post-thaw by phase contrast microscopy. Percent viability was evaluated post-thaw by propidium iodide (PI) and SYBR 14 staining using the LIVE/DEAD Sperm Viability Kit protocol. For the study …
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Subzelluläre Lokalisation des Y-box-Proteins-1 (YB-1) in Mesangialzellen und Charakterisierung der dafür verantwortlichen Proteindomänen
As a DNA- and RNA binding protein the cold-shock protein YB-1 serves multiple functions in the nucleus and cytoplasm, e.g. regulating transcription, RNA-processing, -translation and –storage. In the present study we examined the expression and localisation pattern of YB-1 in various models of …
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The impacts of environmental and anthropogenic stressors on largemouth bass: an integration of field and laboratory studies
… homeostasis during large (12°C) temperature shocks and low (< 4 mg O2/L) levels of dissolved oxygen. Cumulative physiological impacts of multiple cold shocks were only slightly greater than the disturbances sustained during a single cold shock, suggesting largemouth bass are able to tolerate …
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