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Showing 1 to 8 of 8 for “"cofactor regeneration"”.
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Ganzzellbiotransformationen mit rekombinanten Escherichia coli zur Synthese chiraler Alkohole
… enzymes. Due to their internal production of cofactors biotransformation processes using whole cell catalysts can be operated without addition of external cofactors. In this study recombinant Escherichia coli cells were applied as biocatalysts for the reduction of ketones. By the expression of …
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Intensified biocatalysis for production of fuel and chemicals from lipids.
… of novel carbon electrode materials for cofactor regeneration, and reactor design. One potential advantage of using bioelectrochemical method for cofactor regeneration is the possibility to integrate the biochemical process with biofuel cells for simultaneous chemical production and power …
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Laminar Flow-Based Microchemical Systems for Power Generation, Electrochemical Synthesis, and Biological Cell Studies
… fine chemicals by microscale-enabled efficient regeneration of cofactors, as well as for (2) the study of intestinal stem cells on a surface that mimics their in vivo environment. In the first system, the ability to focus reagent streams close to the electrode wall results in a cofactor …
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Beyond bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell biocatalysis
… processing costs, and the need for costly cofactor supplementation. In vivo operation can overcome these issues, but brings its own set of limitations. This thesis presents research on the in vivo biotransformation of n-octane to 1-octanol by CYP153A6 and its natural redox partners, …
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Cloning, expression, charakterization and immobilization of carbonyl reductase from Candida parapsilosis
… avoid stoichiometric amounts, substrate-coupled cofactor regeneration was carried out with 2-propanol as co-substrate in all the systems. The highest product concentration was obtained in a liquid two-phase system with 25% (v/v) hexane as substrate reservoir. The activity and stability of the …
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Prozessentwicklung mit maßgeschneiderten rekombinanten Biokatalysatoren zur Synthese einer Bulk- und einer Feinchemikalie
… of both enzymes enabled self-sufficient regeneration of host internal cofactors, so that high costs for external addition of cofactors or auxiliaries are saved. Buffer screening and selection further improved process economy but also sustainability. The monooxygenase as the limiting …
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Vibrational spectroscopic studies on [NiFe] hydrogenases: insights into structure and function
… biotechnological applications such as NAD(P)H-cofactor regeneration systems and biosensors. Despite these achievements, these enzymes also display a few drawbacks limiting their application. First, they are not easy to produce as their maturation depends on a complex biosynthetic machinery. …
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Stabilitäten der Benzaldehydlyase aus Pseudomonas fluorescens und der Carbonylreduktase aus Candida parapsilosis in wässrig-organischen Zweiphasensystemen
… isopropanol which was used for substrate-coupled cofactor regeneration. CPCR stability in reactive systems with cyclohexane and n-heptane was comparable to stability under non-reactive conditions. Variant CPCR-I79T,V83S showed increased process stability in all three biphasic systems.