Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 86 for “"coat protein"”.
-
Specific Bacteriophage Coat Protein-Rna Interactions
The coat proteins of the group I bacteriophages translationally repress their replicase genes by binding specifically to a region around the initiation codon called the translational operator. A large number of operator variants have been synthesized by using RNA ligase and transcription of …
-
Molecular characterization of the cucumber necrosis virus coat protein gene
… mutant which lacks the coding sequence for the coat protein protruding domain, PD(-), was constructed by site-directed mutagenesis of an infectious CNV cDNA clone, pK2/M5 (wild-type, 4701 nt). Transcripts of PD(-) were infectious in Nicotiana clevelandii, however, the resulting local lesions …
-
Coat Protein -Mediated Resistance of Apple Mosaic Virus in Nicotiana Benthamiana
The detection of protein accumulation in transgenic plants carrying the full-length ApMV-CP and conferring resistance to ApMV suggested that transgenic coat protein accumulation was involved in eliciting the ApMV resistance response. Protein accumulation of lower-truncated ApMV-CP did not induce …
-
Characterization and nucleotide sequence of apple mosaic virus coat protein gene
… nucleotides, respectively. The ORF encodes for a coat protein of a 25,085 Mr. The sequence contains several inverted repeats that could form hair-pin structures. In addition, two palindromic sequence were found in the ORF with a third palindrome in the 3$\sp\prime$-nontranslated region. These …
-
Molecular diversity and coat protein expression of Sweet potato leaf curl virus
… of a fragment of the replication-associated protein gene (AC1). Ten of these begomoviruses were closely related to SPLCV and one was closely related to Sweet potato leaf curl Georgia virus (SPLCGV). These results suggest that in the US, SPLCV may be more common in sweetpotato genotypes than …
-
Characterization of soybean mosaic virus coat protein-mediated resistance in transgenic soybeans
<p>Soybean plants transformed with the coat protein (CP) gene from soybean mosaic virus, strain N, were obtained by using Agribacterium tumefaciens mediated transformation. Polymerase chain reaction and Southern blot analyses were used to detect the transgene. Four insertion events were detected in …
-
Sequence Specific Interaction of R17 Coat Protein With Its Rna Binding Site
The interaction between RNA bacteriophage R17 coat protein and its RNA binding site for translational repression was studied as an example of a sequence-specific RNA-protein interaction. The binding site consists of fewer than 20 contiguous nucleotides which form a small hairpin. The thermodynamic …
-
Identification of Protein-Phosphate Contacts in the R17 Coat Protein Binding Site
Small RNAs of defined length and sequence can be synthesized enzymatically using T7 RNA polymerase and synthetic DNA templates which contain the T7 promoter. The templates are base paired from $-17$ to +1 and contain a long 5$\sp\prime$ overhang. The products of the transcription reactions have the …
-
Transformation of Brassica napus cv. Westar with the beet western yellows virus coat protein gene
… copies of the beet western yellows virus (BWYV) coat protein (CP) gene were synthesized from genomic BVVYV RNA using reverse transcriptase (RT) followed by the polymerase chain reaction (PCR). The ds cDNA copy of the BWYV CP was then cloned into plasmid pRT103, a plant expression vector which …
-
Inheritance and expression of Cry3Aa and PVY-O coat protein transgenes in diploid and tetraploid potato
… lines of potato were developed with a PVY-O coat protein transgene. These plants were challenged with PVY-O and monitored for symptoms visually and for virus serologically. One transgenic line exhibited complete resistance to PVY-O while two others showed a delay in symptom occurrence. …
-
Molecular characterization and nucleotide sequence of the coat protein gene of the NL-3 strain of bean common mosaic virus
… cloned into lambda Uni-ZAP XR vector. The coat protein gene of the potyvirus is located at the extreme 3$\sp\prime$ end of the viral RNA, so clones of approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing …
-
Plant Virus Diagnostics: Comparison of classical and membrane-based techniques for immunoassay and coat protein sequence characterization for Cucumber mosaic virus and three potyviruses
Diagnostics is important in the development and implementation of pest management strategies. The virus diagnostic capabilities of several plant pathology collaborators within the Integrated Pest Management Collaborative Research Support Program (IPM CRSP) host countries were evaluated with the aid …
-
Electron microscopic studies on barley yellow dwarf virus infection in oats
… the subcellular accumulation of two viral proteins, the coat protein and a single strand RNA binding protein (17 kDa protein), and the viral RNA. Viral coat protein was first detected in the cytoplasm surrounding clusters of filamentous material that was shown to contain viral RNA. It is …
-
A study of the molecular variability of some South African isolates of tobacco necrosis virus
… by NA hybridization with probes derived to the coat protein of an A- (TNVWheat) and a D-type (TNV-Papaya green lesion) isolate. RT-PCR with A and D specific primers did not amplify the coat protein of three A- and D-type TNV isolates which appears to indicate that the detection by PCR with …
-
Biochemical and Genetic Analyses of Rna-Protein Interactions
The coat protein from bacteriophage R17/MS2 functions to repress translation of replicase and functions to encapsidate the RNA genome of the phage. The coat protein accomplishes these functions by interacting with a 21 nucleotide stem-loop structure located within the translation initiation region …
-
Investigations into the use of maize streak virus as a gene vector
… cassette in place of the viral movement and coat protein genes were used to generate transformed maize cell lines. A high proportion of these contained MSV-based episomes at high copy number. However, embryogenic maize tissue of the Hill line was not regenerable when an MSV-based replicon was …
-
The mechanism of component recognition of bipartite geminiviruses: Genetic studies of the replication of squash leaf curl virus, and, Genetic and functional studies of squash leaf curl virus coat protein AR1
… for efficient viral replication; (3) replication protein AL1 interacts with the sequence between the directly repeated motif and the stem-loop structure to balance replication of the two viral components; (4) balanced replication of the two viral components constitutes the molecular basis of …
-
Protein-Protein and Protein-Rna Interactions in the in Vitro Assembly of the R17 Bacteriophage
… infects male strains of E. coli. The role of RNA protein interactions in the in vitro assembly of the virus is examined in order to determine both what features of the RNA are important for its selective packaging by R17 coat protein and how the polymerization of the coat protein into the T = 3 …
-
Assessing TMV as an immunogenic particle : the expression of HIV-1 subtype C V3 loop neutralizing antibody epitopes on the surface of TMV virions
… were expressed on the surface of the TMV coat protein, which served as an ideal means of antigen display. This model antigen display system was capable of assembling into multivalent, highly repetitive structures thereby displaying many copies of the attached epitope in the assembled …
-
Cloning and characterization of the 3' terminal regions of RNA from select strains of maize dwarf mosaic virus and sugarcane mosaic virus
… of the coding region of the nuclear inclusion II protein, the complete coat protein cistron, and the complete untranslated region of these viruses plus a poly-adenylated 3$\sp\prime$-terminal tail. Nucleotide sequence analyses of each of these cDNA inserts have been determined. Homology studies …
Page 1 of 5