Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"coactivators"”.
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Regulation of Anaphase Promoting Complex Coactivators
… substrates by the evolutionarily conserved coactivators Cdc20 and Cdh1. After an initial wave of APC-Cdc20 activity, APC-Cdh1 degrades multiple mitotic proteins from mitotic exit through G1; inhibitory phosphorylation of Cdh1 by CDK and Polo kinase may allow accumulation of Cdh1 targets in …
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Interactions of Steroid Hormone Receptors With Coactivators and DNA
… response elements (HREs) on DNA, recruiting coactivators and then chromatin remodeling complexes and ultimately the basal transcription machinery. In vivo data suggests that the components of transcription complexes at HREs undergo rapid exchange and replacement. Using our Fluorescence …
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Preliminary Study on How Tumor Suppressor Nf2 Inhibits Transcriptional Coactivators Yap/Taz in the Developing Mouse Brain
… the Hippo pathway effectors, transcriptional coactivators Yap/Taz. The molecular mechanism of how Nf2 regulates Yap/Taz is not understood. Here I showed that Nf2 regulated the Yap/Taz activity by decreasing the stability of Yap/Taz. The regulation was independent of Yap-S366 phosphorylation, …
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Development and Application of a Fluorescence Anisotropy Microplate Assay for Analyzing the Interaction of Steroid Hormone Receptors With DNA and Coactivators
… transcription is the recruitment of P160 coactivators by ER. The FAMA represents the first reliable in vitro assay for the binding of full-length P160 coactivators to the ERE-ER complex. Binding of full-length SRC1a to the ERE-ERalpha and ERE-ERbeta complex occurred only in the presence of …
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Structural Characterization and Chemical Inhibition of the ARNT/TACC3 Complex
… studies of a new group of transcriptional coactivators (coiled-coil coactivators: TACC3, TRIP230, CoCoA), involved in cancer development and progression. Normally, these coactivators play an essential role in the HIF hypoxia response, directly interacting with the ARNT subunit of HIF in a …
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Enhancers and phase separation in the control of gene expression
… which are bound by transcription factors and coactivators, leading to the recruitment of RNA polymerase II and the production of RNA. Enhancers are thought to loop to specific gene promoters to stimulate transcription, but the mechanisms that cause enhancers to selectively loop to specific …
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A four-and-a-half LIM protein FHL2 acts as a coactivator for the Wilms' tumor suppressor WT1 during gonadal differentiation
… the MIS gene. Importantly, so far no bona fide coactivators for WT1 have been described.
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Role of P300 Zz Domain In Chromatin Association and Histone Acetylation
… by numerous factors including transcription coactivators. The p300 protein and its close paralogue CREB-binding protein (CREBBP, aka CBP) are well-known transcriptional coactivators that have intrinsic lysine acetyltransferase activity. The functions of p300/CBP largely rely on their …
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STAT3-protein interactions in IL-6/gp130 signaling
… IL-6 signal transduction by interacting with its coactivators. First, the function of an IL-6 inducible complex of STAT3 with cyclin-dependent kinase 9 (CDK9) was examined by using gamma-Fibrinogen (ã-FBG), an acute phase protein, as a model. IL-6 induces a strong nuclear association of STAT3 with …
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Regulation of SRC-3 localization and dynamics by phosphorylation during ERα-dependent transcriptional activation
… these factors involves either the recruitment of coactivators or corepressors and direct interaction with the basal transcriptional machinery (1). Hormone-activated nuclear receptors (NRs) are well characterized transcriptional factors (2) that bind to the promoters of their target genes and …
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UNRAVELING TAZ REGULATION IN BREAST CANCER BY CRISPR SCREENING
YAP and TAZ are transcriptional coactivators belonging to the Hippo signaling pathway. Their activity is regulated by a complex network of upstream signals. As consequence, they orchestrate a wide spectrum of downstream cellular responses. In physiological conditions, a precisely balanced …
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Phenotypic screening and AI uncovers modulators of YAP and TAZ activity in glioblastoma
… for GBM. The main Hippo pathway transcriptional coactivators YAP and TAZ are master regulators of up to 70% of the entire glioma stem cell gene signature and so represent key molecular targets in the development of new therapeutic strategies for GBM. However, targeting these transcription …
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Regulation of Pyruvate Dehydrogenase Kinase 4 by Thyroid Hormone / Role of Peroxisome Proliferator Activated Receptor Gamma Coactivator-1 alpha and Ccaat Enhancer Binding Protein
… thyroid hormone receptor (TR), transcriptional coactivators especially the peroxisome proliferator activated receptor gamma coactivator-1 (PGC-1α) and other transcription factors that act as accessory factors in T3 actions. </p> <p>Thyroid hormone receptors (TRα or TRβ) are part of the nuclear …
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The Effect of HACE1 on RAR Protein Stability
… in the presence of ligand with the aid of coactivators. The mechanism of the ligand independent action of the AF-1 domain is less clear. The AF-1 domain of RARs may be regulated by interacting proteins such as HACE1. In vitro and in vivo studies in our lab have shown that HACE1 interacts …
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The Role of YAP/TAZ in von Willebrand Factor Gene Expression
… in venous regions. YAP and TAZ are paralogous coactivators largely known for their role in the Hippo pathway. They function predominantly through interactions with TEAD transcription factors to regulate transcription of target genes, which ultimately execute the physiological output of upstream …
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Estrogen Response Element and the Promoter Context of the Human and Mouse Lactoferrin Genes Influence Estrogen Receptor alpha-Mediated Transactivation Activity in Mammary Gland Cells
… and that the ERRE influenced the selectivity of coactivators in liganded ER-alpha-mediated transcriptional activity. Like the lactoferrin gene ERE, most known natural estrogen response elements are imperfect palindromes that differ from the consensus by at least a 1 base pair change and confer …
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Characterizing the Mechanisms Regulating Enhancer Commissioning by MyoD in Muscle Cells
… with mass spectrometry to identify potential coactivators of MyoD. Our results reveal a link between MyoD and the Mediator complex, a key component of enhancer-promoter looping. Next, using phenotypic assays and transcriptomics, we demonstrate that knocking down Mediator function severely …
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Estrogen Receptor Ligands and Regulation of Gene Expression
… due to ligand-differential recruitment of ER and coactivators; (2) EREs mediate both E2 and tamoxifen effects, while certain EREs preferentially mediate the E2 effect, presumably dependent on the ERE sequence and promoter context. These findings provide new insight about gene regulation by …
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Molecular Mechanisms Underlying The Transcriptional Regulation of T Helper 17 and Regulatory T Cells
… the association of the nuclear receptors to coactivators or to recruit histone deacetylases to RORa- or RORgt-target genes. Since TGF-b is a common cytokine required for the commitment towards both Th lineages, we determined the role of the TGF-b-dependent signaling pathway in the generation …
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Roles of Myocardin-Related Transcription Factors in Muscle and Brain
… Myocardin Related Transcription Factor (MRTF) coactivators during development. I present here my studies on the functions of MRTFs during brain and muscle development. First, I show that MRTF-A and -B redundantly control neuronal migration and neurite outgrowth during brain development. …
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