Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"co-transcriptional"”.
-
Co-transcriptional recruitment of the U1 snRNP
… of most pre-mRNAs occurs, at least in part, co-transcriptionally. In order to validate this principle in yeast and establish an experimental system for monitoring spliceosome assembly in vivo, I have employed the chromatin immunoprecipitation (ChIP) assay to study co-transcriptional splicing …
-
On co-transcriptional splicing and U6 snRNA biogenesis
… a cap structure is added to its 5’ end, noncoding, intervening sequences (introns) are removed and coding exons are ligated together and a poly(A) tail is added to its 3’end. Splicing, the process of intron removal, is carried out in the spliceosome, a megacomplex comprehending up to 300 …
-
Mechanisms of piRNA-guided co-transcriptional gene silencing
… at these sequences prevents ectopic recombination and TE mobilisation. In *Drosophila* ovaries, this depends on Piwi, which is guided by a bound small RNA to nascent TE transcripts where it regulates the formation of transcriptionally repressive chromatin states. As well as numerous …
-
HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
RNA interference (RNAi) is a post-transcriptional gene silencing mechanism found in all eukaryotic organisms. It is characterized by a family of small non-coding RNAs, either endogenous (in the case of microRNAs) or exogenous (in the case of siRNAs), that inhibits gene expression …
-
Mechanisms of piRNA biogenesis and co-transcriptional silencing of transposable elements in Drosophila
A large fraction of eukaryotic genomes consists of mobile, repetitive elements called transposons. Since their uncontrolled mobilisation is a potentially harmful event, several molecular mechanisms have evolved to counteract transposon activation and thus safeguard genome integrity. Among these is …
-
Placental co-transcriptional activator Vestigial-like 1 (VGLL1) drives tumorigenesis via increasing transcription of proliferation and invasion genes
<p>Vestigial-like 1 (VGLL1) is a co-transcriptional activator that binds to TEA domain containing transcription factors (TEADs). Its expression is upregulated in a variety of aggressive cancer types, including pancreatic and basal-like breast cancer, and increased transcription of VGLL1 is strongly …
-
Multiple regulatory layers in the establishment of Rbfox2 splicing networks
… is mediated by RNA binding proteins (RBPs) recognizing short sequence motifs in nascent transcripts. The Rbfox RBPs are highly conserved splicing factors that regulate tissue-specific exon inclusion by binding the RNA motif UGCAUG. We sought to comprehensively define the Rbfox2 splicing …
-
Making Ribosomes: Biochemical and Structural Studies of Early Ribosome Biogenesis in Yeast
<p>The ribosome is a complex macromolecule responsible for the synthesis of all proteins in the cell. In yeast, it is made of four ribosomal RNAs and 79 proteins, asymmetrically divided in a small and large subunit. In a growing yeast cell, more than 2000 ribosomes are assembled every minute. The …
-
IDENTIFICATION OF SPLICING PATHWAY MUTATIONS VIA TARGETED SEQUENCING
… the spliceosome is an essential and regulated component of eukaryotic gene expression. While ever-increasing numbers of human genetic diseases can be linked to defects in the splicing pathway, our molecular understanding of how these mutations disrupt this complex process remains incomplete. To …
-
Vestigial-Like 1 Is A Shared Targetable Cancer-Placenta Antigen Expressed By Pancreatic and Basal-Like Breast Cancers
… HLA-A*0101 restricted peptide derived from co-transcriptional activator Vestigial-like 1 (VGLL1), a novel putative TAA demonstrating overexpression in multiple tumor types and low or absent transcript expression in normal tissues with the exception of placenta. VGLL1-specific CTL isolated …
-
Spliceosomal small nuclear ribonucleoprotein particle trafficking and maturation in the nuclear compartment
… fluorescein-labeled spliceosomal snRNAs, in the context of functional snRNPs, are targeted to elongating pre-mRNAs. This finding presents us with a novel assay with which to dissect the mechanism by which snRNPs are targeted to nascent pre-mRNA transcripts. Two critical advantages offered by this …
-
14-3-3 Zeta Overexpression Serves As A Novel Molecular Switch Turning Tgf-Beta From Tumor Suppressor to Tumor Promoter
… However, the role of TGF-β in cancer is complicate. TGF-β has primarily been recognized as tumor suppressor, because it can directly inhibit cell proliferation of normal and premalignant epithelial cell. However, in the last stage of tumor progression, TGF-β functions as tumor promoter to …
-
Chromatin Control of the Antiviral Response to Influenza
… for our understanding of the mechanisms that control gene expression has been the finding that the histones recruit proteins with effector functions to chromatin. This is mediated primarily by post-translational modifications that occur on the histone N-terminal domains ("tails"). Single or …
-
Single-Molecule Characterization of RNA via Nanopore Sensing
… RNA characterization methods often provide an incomplete picture of transcript populations. Some approaches fail to resolve transcript heterogeneity, while those that offer broad overviews typically struggle to capture sequence-specific features or detect chemical modifications which can …
-
Defining the molecular basis for the β-catenin and CDC73 interaction
… (Pol II) is required for expression of protein coding genes. To initiate gene transcription, Pol II is recruited to gene promoters by transcription factors. Gene specific transcription factors, including β-catenin, a component of the Wnt signaling pathway, are used to regulate gene expression in …
-
Uncovering Signalling Pathways Mediating Gastrointestinal Morphogenesis and Tumorigenesis
… gastrointestinal development requires precisely coordinated intercellular communication via signalling pathways, mechanisms underlying intestinal morphogenesis remain unclear. The Hedgehog signalling pathway is critical for many aspects of gut development, however, its downstream transcriptional …
-
piRNA-dependent transcriptional gene silencing during Drosophila oogenesis and embryogenesis
… In Drosophila melanogaster ovaries, piRNA-Piwi complexes localise to the nucleus and scan nascent transcripts for transposon expression by using complementary antisense piRNAs as guides. Following target engagement, the gonad-specific protein Panoramix (Panx) is recruited and induces …
-
The Role of Maelstrom in the Drosophila piRNA Pathway
… portion of eukaryotic genomes and their uncontrolled activity can lead to genomic instability. In Drosophila ovaries, silencing of these elements is tightly regulated through the establishment of heterochromatin which prevents TE mobilisation. Piwi guided by piRNAs to nascent TE …
-
ELUCIDATION OF REGULATORY MECHANISMS OF TRANSCRIPTION AND COUPLED mRNA EXPORT
Gene expression is driven by the combined actions of numerous proteins that act in a regulated fashion. One of the central processes in gene expression is the export of the synthesized mRNA from nucleus to the cytoplasm where it gets translated into proteins. This journey of mRNA is mediated by …
-
The Role of Hippo Signalling in Merlin Null Tumours of the Nervous System
… The Hippo pathway is an evolutionarily conserved pathway that has been shown to control tissue size by contactmediated growth inhibition. Previous studies have shown that there is aberrant nuclear activity of yes-associated protein (YAP) and transcriptional coactivator with PDZbinding …
Page 1 of 2