Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"co-localisation"”.
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Defining the functional role of MAGE-C1 in Multiple Myeloma
… and techniques to study MAGE proteins by comparing MAGE-C1 to better described CTAs: MAGE-C2 and MAGE-A1. Firstly, the expression of MAGE-C1, MAGE-A1, MAGE-C2 and their respective binding partners, Ski interacting protein (SKIP) and Ring-box 1 (RBX1), was confirmed in RPMI-8662. Next, …
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Palmitoylation and Glutathionylation of Key Sarcolemmal Signalling Proteins in Cardiac Muscle
… Ventricular Myocytes (ARVM) under physiological conditions, glutathionylated proteins were identified using LC-MS/MS and cross referenced with the palmitoylproteome of ARVM. This identified 129 proteins that are both palmitoylated and glutathionylated in ARVM.<br/><br/>Novel glutathionylated …
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Neuropeptide hormones from the eyestalks of Jasus Lalandii
The X-organ sinus gland complex, situated in the eyestalks of decapod crustaceans, are known to be a source of a variety of neuropeptide hormones that regulate a number of diverse physiological processes. This neuroendocrine complex was investigated in 3 crustacean species, viz. the European shore …
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Developing a selective sphingosine-1-phosphate-5 (s1p5) radiotracer to image oligodendrocytes using preclinical positron emission tomography (PET)
… be of particular interest as S1P5 is a G-protein coupled receptor and agonists show a bias to binding receptors in the active state. If successfully translated, these ligands could aid in improving trials of novel therapeutics, improving assessment of disease progression and importantly furthering …
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Investigation of conserved Flagellum proteins in Trypanosoma brucei
… More than 300 proteins have been identified as components of the T. brucei flagellum but functional analysis of the majority of these proteins has not been carried out to date. This project used a bioinformatics approach to identify potential flagellum proteins in T. brucei that were also …
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A genomic investigation into gastrointestinal antimicrobial resistance and clinical infection in patients undergoing transplantation
… barrier to the improvement of transplant outcomes worldwide. My thesis re-examines the significant body of research that encompasses gut-origin sepsis and contextualises it within the ongoing AMR crisis. I combined whole-genome and metagenomic sequencing approaches to 1) characterise …
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GPR50, a potential factor involved in psychiatric disorders interacts with Alzheimer’s disease-related protein β- secretase (BACE1)
GPR50, an X-linked orphan G protein-coupled receptor (GPCR), is a risk factor for bipolar disorder (BD) in female subjects. It has been shown that GPR50 plays a part in neurite outgrowth, glucocorticoid receptor signalling and leptin signalling by interacting with major factors involved in these …
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INVESTIGATION OF THE DISTAL APPENDAGE PROTEIN CEP164 IN Trypanosoma brucei
Cilia and flagella are highly conserved organelles required for eukaryotic cells to perform sensory and motility functions. They are found in many cells of the human body and are essential for the survival of single celled protozoans. These organelles are nucleated from a basal body after docking …
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Central Expression of Sex Steroid Receptors during the Ring Dove (Streptopeliarisoria) Breeding Cycle
… of male and female ring doves. Nuclear AR-ir in courting birds was widespread throughout the telencephalong diencephalon, and mesencephalon. Nuclear PR-ir was only localised in discrete regions of the preoptic-hypothalamus of both sexes. In the anterior and posterior hypothalamus, high density of …
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Characterisation of Nuclear EnvelopeAssociated Proteins (NEAP) in Arabidopsis thaliana
… wrapping DNA around histone proteins. The NE is composed of two membranes: on the nucleoplasmic side, the Inner Nuclear Membrane (INM) and on the cytoplasmic side, the Outer Nuclear Membrane. The NE allows communication between both compartments through Nuclear Pore Complexes and bridges the …
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Understanding the pathogenesis of myotonic dystrophy type 1
… the full range of targets and the pathogenic consequences, we sought to mimic the pathogenesis of myotonic dystrophy type 1 with temporal and spatial control: temporal to reproduce the developmental pathogenesis of the congenital form, and spatial to isolate tissue specific pathology. To do …
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Investigating the role of Klhl31 in striated muscle
… in the somites and is slightly delayed compared to the expression of early myogenic regulatory factors. With this study we wanted to analyse the function of Klhl31 during myogenesis in more detail. Using C2C12 mouse myotubes as our model cell line to study myogenic differentiation and …
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CRAMP1 drives linker histone expression to enable Polycomb repression
The essential role of the core histones (H2A, H2B, H3 and H4) in DNA packaging has been well-understood for decades, but the function of the linker histone (H1) remains enigmatic. H1 compacts nucleosome arrays in vitro, but the multiple H1 subtypes and variants encoded in mammalian genomes (11 in …
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Production, characterisation and use of fluorescent markers in the study of plant nuclear envelope dynamics
… least studied membranes in plant cells. Genes encoding NE protein homologues are absent from currently available sequenced plant genomes. To produce a specific marker for the plant NE and in view of previous positive results with heterologously expressed proteins in plant cells, mammalian NE …
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Design and Application of Probes of Novel Bioactivities
… relatively unexplored. Since the plant kingdom contains a greater diversity of potential biocatalysts this represents an untapped resource. Two simple plant enzyme systems were investigated: AtSFGH and AtCXE12. A series of profluorescent esters based on 4 methylumbelliferone and fluorescein were …
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The neurobiology of central dopamine and progesterone in the reproductive cycle of the ring dove (Streptopelia risoria)
… posterior thalami in female brooding birds. This confirms that increases in dopamine activity during the brooding period are exhibited in regions integrating neuroendocrine and sensory information to mediate the expression of parental defence behaviours. Immunofluorescence studies revealed no …
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The role of tissue-resident immune cells in PanIN to PDAC progression
… although their role in PDAC remains contentious with both pro- and anti-cancer roles having been described. In this thesis, I have characterised the role of ILC2 in early- and late-stage PDAC with specific focus on how ILC2 interact with stromal and immune components in the …
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An Investigation into the Intracellular Trafficking and Processing Pathways of Amyloid Precursor Protein
… disease (AD) pathology. It undergoes a complex intracellular trafficking and processing itinerary to produce toxic amyloid-β peptides that accumulate in extracellular plaques in the brains of AD patients. Its cleavage is dependent on several different secretase enzymes that are …
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MECHANISM AND STAGES OF PACKAGING OF VP8, THE MAJOR TEGUMENT PROTEIN OF BOVINE HERPESVIRUS-1
… by pUS3. VP8 (pUL47) phosphorylation mutant contains a significantly lower amount of VP8 (pUL47) (~30%) than wild type virus. Outside the context of infection, VP8 (pUL47) is translocated to the cytoplasm if co-transfected with pUS3 encoding plasmid, but remains cytoplasmic and is not …
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Therapeutic potential of targeting group III metabotropic glutamate receptors as a disease modifying strategy in the treatment of Parkinson’s disease
… subthalamonigral pathway may underlie this continual degeneration of the nigrostriatal system. With this in mind, this thesis examined whether selective activation of group III metabotropic glutamate receptor subtypes may offer a novel strategy to halt persistent degeneration in …
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