Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 62 for “"co-culture system"”.
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Investigating the movement of intestinal intraepithelial lymphocytes using an organoid co-culture system
… is the main site of nutrient absorption. It is continuously exposed to food antigens, and also several bacteria and microorganisms. Intestinal intraepithelial lymphocytes (IELs) are T cells that are interspersed between epithelial cells, above the basement membrane. This makes them one of the …
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Sea-based Integrated Multi-Trophic Aquaculture: Investigation of a fish, bivalve and macroalgal co-culture system.
Sea-based integrated multi-trophic aquaculture (sIMTA) was explored as an amalgam of the processes of polyculture and biofiltration, with the primary objectives of improved aquaculture production efficiency and wastes remediation. The study established a coculture of Atlantic salmon (Salmo salar, …
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Enhanced biohydrogen production and substrate utilization by co-culture fermentation with reduced extracellular electron shuttles
… due to no greenhouse gas (GHG) emission during combustion and the highest conversion efficiency in fuel cells and highest energy content per unit mass compared to carbon-based energy carriers. However, three key challenges for large-scale biohydrogen production are to increase i) the hydrogen …
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Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri
… shows the cytopathic effects against primary cultured rat microglial cells, the morphological changes of microglial cells was observed by a light, scanning and transmission electron microscopes. And then, the cytotoxicity of N. fowleri lysate against rat microglial cells was also observed by …
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Activation and Maintenance of Intestinal Intraepithelial Lymphocytes (IELs)
… immunological task. The intestinal immune system needs to be tolerant towards nutrients and microbiota present in the intestinal lumen, while simultaneously detecting and responding to dangers such as pathogens. A single-cell layer of intestinal epithelial cells (IECs) acts as a first line …
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An in vitro investigation into the pigmentary phenotype of melanocytes and keratinocyte co-cultures to improve wound healing
… prominent in dark-skinned individuals and could result in serious psychosocial consequences such as low self-esteem, stigmatisation and discrimination among sufferers. Methods aimed at investigating rapid and efficient repigmentation in wounded skin are therefore pertinent. The aims of this …
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The effect of temperature on the bystander effect as examined in human prostate carcinoma cells with alpha particle irradiation
… non-irradiated cells. Previous studies in the Coderre lab showed that the soluble factor released by DU-145 human prostate carcinoma cells was a short-lived, free radical species (Wang and Coderre, Rad. Res., 164, 711-722, 2005). This thesis examined the effect of temperature on the bystander …
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Development of 3-d liver model for drug screening applications
The need for a an in vitro drug screening system that mimics the structural organization of natural liver for multi-functionality and maintenance of hepatocytes has become vital in the drug development process. The objective of this study was to engineer a three-dimensional (3-D) liver model using …
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Direct and indirect effects of alpha-particle irradiations of human prostate tumor cells
… of this project is to establish a model system to study the direct effect, the bystander effect and the combinational effect of alpha-particle irradiations of human prostate tumor cells, toward the goal of improving the effectiveness of alpha-particle involved radiation therapies such as …
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Development of a hydrogel-based biocompatible platform for studying metabolic interactions between algae and bacteria
… and to understand nutrient cycling in ocean ecosystems. To date, studying chemical exchange between these microbial species based on molecular diffusion processes has been challenging due to a lack of an appropriate co-culture system. In this thesis, a hydrogel-based biocompatible platform is …
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Role of Laminins in the Development of the Peripheral Nervous System
… signals. In a Schwann cell/dorsal root ganglion co-culture system, disruption of laminins impairs podia formation as well as the elongation of Schwann cells. These results demonstrate that: 1) laminins initiate axonal sorting and mediate axon-Schwann cell interactions required for Schwann cell …
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Novel mechanisms of endothelial-epithelial interactions underlying cancer metastasis
… individual pathways in the metastatic cascade could lead to development of metastasis-specific therapeutics; however, current in vivo metastasis model systems are not efficient tools for isolating a single molecular event from the network of complex biological pathways. In response to these …
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Regulation of macrophage subsets in homeostatic and inflammatory mucosal environments
… cells and macrophages is integral to mucosal immune fate: determining the decision between tolerance and immune activation/inflammation. Endotoxin tolerisation (ET) is a circumstance where cells go through a hypo-responsive state, unable to respond to further endotoxin-LPS challenge. …
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Effects of Diethylstilbestrol on Murine Early Embryonic Stem Cell Differentiation Using an Embryoid Body Culture System
Objectives: The effects of estrogens on immune system formation and function are well documented. Diethylstilbestrol (DES), a synthetic estrogen, has been linked to neoplasia and immune cell dysfunction in humans and animals exposed in-utero. In-vitro effects of DES exposure of murine embryonic …
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Cytopathic Effects of a Pathogenic Naegleria fowleri and an Anti-Nfa1 Antibody on Rat Microglial Cells
… On the pathogenic mechanisms of N. fowleri concerned with host tissue invasion, the adherence of amoeba to host cells is most important. The nfa1 gene (360 bp) was previously cloned from a cDNA library of pathogenic N. fowleri by immunoscreening, and produced a 13.1 kDa recombinant protein …
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hiPSC-Derived Cerebral Small Vessel Disease Models
… the blood brain barrier (BBB) is disrupted and could contribute to increased BBB permeability. Evidence also shows a role of the extracellular matrix (ECM) in the progression of cSVD pathology. We generated *in-vitro* co-culture models using human induced pluripotent stem cells (hiPSCs) with …
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Genetic programming of human iPSC-derived macrophages provides a tool to study the Erythroblastic Island niche in vitro
… have been hampered due to the ethical constraints and technical difficulties related to their isolation/derivation for in vitro expansion, and to the difficulty of genetically modifying them to interrogate the role of specific factors in macrophage behaviour. Thus, an off-the-shelf …
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Placental co-culture model reveals Listeria monocytogenes utilization of monocytes for vertical transmission
… barrier and infect the fetus, leading to congenital disabilities or death. Extracellular L. monocytogenes infection of the placenta has been well studied; however, the bacteria have not been observed translocating this barrier intracellularly. As an intracellular pathogen, L. monocytogenes …
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Use of gene expression to characterize heterogeneous liver cell populations
… in vivo liver function. A variety of in vitro culture systems have been developed to address different aspects of liver physiology and architecture in order to recreate the microenvironment. These in vitro co-culture strategies have been limited by their inability to systematically characterize …
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Spatially controlled engineering of myocardial tissue
… cues to direct the assembly of cells into complex, organized tissues. Microfabrication methods provide analogous technologies to accurately control the displacement of proteins and cells in vitro to mimic their natural spatial organization. Thus, we have investigated how spatially …
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