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Showing 1 to 20 of 47 for “"clostridia"”.
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Genetic Characterization and Manipulation of Solvent -Producing Clostridia
The sol operons from Clostridium beijerinckii NCIMB 8052 and C. beijerinckii BA101 were cloned and sequenced to determine if differences at the sequence level were responsible for the increased butanol production exhibited by C. beijerinckii BA101 relative to the wild type C. beijerinckii NCMIB …
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Genetic Manipulation and Characterization of Solvent-Producing Clostridia
… done on the formulation of a new solventogenic clostridial fermentation medium based on the use of corn steep liquor (CSL). CSL is a by-product of the corn wet-milling industry, and has been used primarily as a feed supplement in the livestock industry. This nutrient-rich medium is an ideal base …
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The role of clostridia in Bantu beer fermentations
1. The occurrence of clostridia in various stages of Bantu beer production was investigated in order to determine their significance as spoilage organisms in the brewing process and in the final product. 2. The ecology of clostridia was studied by investigating the selective factors of the …
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Properties and Role of Butyrokinase in Energy Conservation by Butyric Clostridia
Made available in DSpace on 2014-12-05T21:45:38Z (GMT). No. of bitstreams: 1 6206246.pdf: 2369028 bytes, checksum: b7ea0492f4659a845acb495b10a34632 (MD5) Previous issue date: 1962
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Development of genetic systems for Clostridia spp. by utilization of extrachromosomal DNA elements
An Escherichia coli-Clostridium perfringens shuttle vector (pAK201) was constructed by ligating a 3.6 Kb fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 Kb). Plasmid pKA201 is 8 kb and codes for resistance to chloramphenicol in E. coli and C. perfringens. Plasmid pAK201 was …
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Functionalization of phage endolysins to treat infections of spore-forming Clostridia bacteria in the era of antibiotic resistance
Certain *Clostridia* species are notable pathogens, not only because they form highly resistant endospores but because they produce toxins and exhibit antibiotic resistance. *Clostridioides difficile* is a nosocomial pathogen responsible for thousands of deaths every year, whereas *Clostridium …
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Psychrophilic and psychrotrophic Clostridium spp. associated with meat spoilage
… exists between psychrophilic and psychrotrophic clostridia and the production of abundant gas in vacuum-packed chilled meats. The attempt was then made to identify these clostridia using conventional microbiological methods. Genotypic approaches to differentiation of psychrophilic and …
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Community Composition and Function of the Hindgut Microbiome in Seaweed-Eating Marine Fish
… at the species level, and Bacteroidia and Clostridia dominated the gut community. Genes encoding endo-acting carbohydrate-active enzymes (CAZymes) associated with both classes suggest their involvement in initiating glycan depolymerization. Bacteroidia contributed the most to CAZyme-related …
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The distribution of anaerobic bacteria along a soil drainage catena
… exception of a sharp rise in early spring. The clostridia constituted at least one third of the obligately anaerobic bacteria present on the poorly drained soil. A sizeable percentage of the obligate anaerobic isolates on this site were either clostridia which formed spores unable to germinate …
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Gas chromatographic analysis of amines and other compounds produced by several species of Clostridium
… flame ionization detectors. Sixty-two strains of clostridia, representing 13 species, were analyzed for soluble products in whole cultures. Many amines were found and total amine concentrations exceed 0.5% (weight) in cultures of several species examined. Neutral products were found to be major …
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The Cloning of a Putative Regulatory Gene and the sol Region from Clostridium beijerinckii
The solvent-producing clostridia are well known for their ability to produce acetone, butanol and isopropanol in industrial fermentation. Production of these compounds occurs in cells that have completed a metabolic switch under specific growth conditions. Knowledge of the regulation of the …
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Molecular Characterization and Utilization of the CAK1 Filamentous Viruslike Particle Derived From Clostridium Beijerinckii NCIMB 6444
… for genetic engineering the solventogenic clostridia in order to improve the utilization of more complex substrates and for the production of specific end-products.
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DELINEATING GUT-MEDIATED RESPONSE AMONG MELANOMA PATIENTS UNDERGOING ANTI-PD1 IMMUNOTHERAPY
… gut microbiota taxa in CR, comprising mostly Clostridia taxa, which also associate with markers for stability and positive systemic state. At the gut functional level, our data demonstrate a key role for Lachnospiraceae-carried flagellin genes in driving a productive anti-tumor response. …
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Epidemiology and Pathophysiology of Clostridial Dermatitis (Cellulitis) in Turkeys
Clostridial dermatitis (CD) is a multifactorial disease of rapidly-growing turkeys. Clostridium septicum (Cs) has been identified as the primary cause, although C. perfringens (Cp) has also been implicated. Pathogenesis is not fully understood; however, it is hypothesized that Clostridia …
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Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262
… the importance of strain differences amongst the Clostridia. Two well defined physiological phases, the acidogenic phase and the solventogenic phase, which characterise the industrial ABE fermentation process were observed in CAMM.
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Genetic Analysis of Clostridium Acetobutylicum Using Two-Dimensional PFGE to Construct a Macrorestriction Map of ATCC 824 and Restriction Enzyme Profiles for Strain Analysis
… B-593 were placed in individual groups. The four Clostridia groups displayed very low restriction enzyme profile similarities. The C. acetobutylicum ATCC 824 chromosome was further characterized with a macrorestriction map consisting of 92 restriction sites and seventeen genes. The …
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
The clostridia are gram-positive, spore-forming, anaerobic pathogens capable of causing various diseases in both humans and animals. To do so, clostridial pathogens secrete a number of lethal toxins each associated with specific diseases. One family of proteins secreted by the clostridia are the …
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Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262
… reduced to elicit a bactericidal effect. In the clostridia, some of the electron transport proteins that provide the source of electrons for the reductive activation of metronidazole play a key role in electron distribution, which in turn regulates the direction of carbon flow in the cell. The …
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Production of 1-butanol and butanol isomers by metabolically engineered Clostridium beijerinckii and Saccharomyces cerevisiae
… fermentation by solventogenic clostridia such as Clostridium beijerinckii. Although the ABE fermentation is considered as a promising process for butanol production, current drawbacks in the ABE fermentation include low titer, yield, and productivity of solvent production that …
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