Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"clonogenic survival assays"”.
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Induced Cytotoxicity In Crebbp/Ep300Mut Head and Neck Squamous Cell Carcinoma
… lines HN31 and FADU were used in cell culture. Clonogenic survival assays were used to cell growth and death with the different agents (DOX, CDDP, XRT with or without A485) used in single and combination treatments. Flow cytometry was used to measure cell death and apoptosis. Cell Titer Glo was …
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The role of the human endonuclease VIII-like 1 and 3 in the repair of DNA replication blocking lesions
… cell fractionation and in vitro ubiquitination assays. TRIM26 RNAi in U2OS cancer cells increased steady state levels of hNEIL1/3 and resulted in resistance to the ICL inducing agent cisplatin, determined through clonogenic survival assays. Transient overexpression of hNEIL1/3 complemented the …
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The effect of UVA irradiation on the DNA damage response in a human keratinocyte cell line
… to irradiation with 50 kJ m-2 UVA caused cell survival to decrease in a dose-dependent manner over that seen where HaCaTs were irradiated in the absence of inhibitor pre-treatment, suggesting the involvement of ATM and ATR in processing UVA-induced lesions. The same observation was not seen …
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Targeted Inactivation of APE1 Endonuclease by E96A Mutation Sensitizes Pancreatic Ductal Adenocarcinoma to DNA-Damagin Therapy
… making DNA repair pathways essential for tumor survival. Apurinic/apyrimidinic endonuclease 1 (APE1/Ref-1) is a multifunctional protein with critical roles in base excision repair (BER) and redox regulation of transcription factors. This dissertation tests the hypothesis that selective …
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The role of c-FLIP in regulating resistance to ionizing radiation
… cell death was assessed by caspase activity assays, short-term cell death and clonogenic survival assays. Overexpression of a FLIP mutant which cannot interact with its binding partner FADD, and thus cannot inhibit caspase-8 activation, did not protect cells from IR induced cell death, …
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Physical and biological characterisation of clinically relevant combined MRI-radiation exposures with conventional and nanoparticle contrast agents
… contrast agents was determined primarily through clonogenic survival assays and DNA damage immunofluorescence assays, at both 225 kVp and 6 MV energies, with and without the presence of a 1.5 T static magnetic field. The contrast enhancement was determined for several different CT and MRI scanners …
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Inhibition of PLK1 by p53 and by PLK1-targeted drugs
… DNA-PK was the main kinase involved. Also, in clonogenic survival assays, more surviving colonies were observed in response to PLK1 inhibitor as compared with nocodazole treatment. Interestingly, PLK1 inhibition resulted in recruitment of the 53BP1 (one of the components of the DNA repair …
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Untersuchungen zur Kombinationswirkung von Valproat und Bestrahlung in Bezug auf klonogenes Überleben, Apoptose, Polyploidie und chromosomale Instabilität in Prostatakarzinom- und Prostatahyperplasie-Zelllinien
Valproat kann als Histondeacetylase-Inhibitor einen starken Antitumoreffekt auf Prostata-Karzinom-Zelllinien ausüben. Dieser Effekt ist jedoch nach Entfernung des Valproats wieder reversibel. Andere Histondeacetylase-Inhibitoren konnten in vitro einen radiosensibilisierenden Effekt auf andere …