Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"cleavage and polyadenylation"”.
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The Role of Cleavage and Polyadenylation Spcific Factor 6 (CPSF6) in Hematopoiesis
<p>Cleavage and polyadenylation specific factor 6 (<em>Cpsf6</em>) translocation is frequently reported in APLL, which is Acute Promyelocytic Leukemia (APL)-like acute myeloid leukemia (AML) and other types of blood diseases associated with abnormal hematopoiesis. CPSF6 is a subunit of the Cleavage …
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Interrogating Dux4 Mrna 3′ End Processing
<p>Double Homeobox 4, Dux4, is the leading candidate gene for Facioscapulohumeral Dystrophy (FSHD). FSHD is the third most common muscular dystrophy, and is characterized by progressive muscle weakness primarily in the upper body. In individuals diagnosed with FSHD, Dux4 is inappropriately …
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Cross-regulation and interaction between eukaryotic gene regulatory processes
… of genes is fundamental to all living processes and can be exerted at many sequential steps. We studied several eukaryotic gene regulatory mechanisms with an emphasis on understanding the interplay between regulatory processes on a genome-wide scale. Gene splicing involves the joining of exonic …
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PolyA signals located near 5’ of genes are silenced by a general mechanism that prevents premature 3’ end processing
… at the 3’ end of eukaryotic genes drive the cleavage and polyadenylation reaction to the nascent pre-mRNA. Although these sequences are expected only at the 3’ end of genes, we found that strong polyA signals are also present within the 5’ untranslated regions (UTRs) of many Drosophila …
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Mammalian gene regulation through the 3' UTR
… the stability, localization, translation and other properties of the message. We explored two classes of motifs, microRNA (miRNA) complementary sites and cleavage and polyadenylation (poly(A)) signals, and provide evidence that specific sequence contextual features are important for their …
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Architecture of eukaryotic mRNA 3' end processing machinery and insights into the mechanism of polyadenylation
… is added by a multi-protein complex known as cleavage and polyadenylation factor (CPF). CPF, along with accessory cleavage factors (CF) IA and IB, cleaves the pre-mRNA within the 3' untranslated region (UTR) and adds a poly(A) tail. Previous work has shown that CPF is a fourteen-subunit …
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Mechanistic insights into endoribonuclease activation of the mRNA 3' end processing machinery by Cleavage Factor IA
… efficient export from the nucleus, translation and mRNA stability. Near the 3' end of the nascent transcript, the pre-mRNA is cleaved at specific RNA sequences, releasing the pre- mRNA from the transcribing RNA polymerase II. The new 3' end is subsequently polyadenylated with a tail of ~60 …
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Engineering fluorescent imaging platforms to characterise molecular interactions with HIV-1 capsid
… of novel cofactors binding to capsid demands sensitive and quantitative assays to better understand their underlying binding mechanisms. Here, I have developed three complementary fluorescence microscopy-based platforms for quantitative characterisation of capsid binding analytes using …
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Biochemical and functional characterization of human RNA binding proteins
RNA not only shuttles information between DNA and proteins but also carries out many other essential cellular functions. Nearly all steps of an RNA's life cycle are controlled by approximately one thousand RNA binding proteins (RBPs) that direct RNA splicing, cleavage and polyadenylation, …
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In vitro reconstitution of the human pre-mRNA 3′ end processing machinery and mechanistic insights into endonuclease activation
… steps, including 5′ capping, splicing, and 3′ end processing. The latter entails endonucleolytic cleavage of the nascent pre-mRNA and addition of a poly(A) tail to the resultant free 3′ end. The poly(A) tail then facilitates nuclear export of mRNAs and controls their stability and …
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Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition
… of gene expression in a wide range of organisms and biological processes. Each miRNA guides Argonaute (Ago) protein complexes to target and repress hundreds of genes in a sequence-dependent manner. To identify all targets of miRNA regulation, we performed UV crosslinking and immunoprecipitation …
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Recombinant expression, purification and structural characterization of Saccharomyces cerevisiae RING finger domain using Nuclear Magnetic Resonance (NMR) spectroscopy
… known as RBBP6. This is a 250kDa protein that stands for Retinoblastoma binding domain protein 6. It is classified according to different homologues namely RBQ1, PACT, SNAMA, Mpe-1 and P2P-R depending on the organisms in which they are found. P2P-R (a RING finger containing protein) was found to …
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A Semi-Supervised Predictive Model to Link Regulatory Regions to Their Target Genes
… processes. In an effort to better understand the process of gene regulation, two predictive machine learning models specifically tailored for analyzing gene transcription and polyadenylation are presented.</p><p>Transcriptional enhancers are specific DNA sequences that act as ``information …
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SAM Homeostasis Is Regulated by CFIm-Mediated Splicing of MAT2A
… as a regulator of MAT2A intron detention and intracellular SAM levels. CFIm25 is a component of the cleavage factor Im (CFIm) complex that regulates poly(A) site selection, but we show it promotes MAT2A splicing independent of poly(A) site selection. CFIm25-mediated MAT2A splicing …
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Posttranscriptional regulation with regard to cleavage factor I (CFI) associated alternative polyadenylation and methyltransferase A (MTA) assisted m6A modifications in Arabidopsis thaliana
… of genes, posttranscriptional modifications and other regulatory pathways enable rapid adaption to both mild and extreme conditions in all domains of life. Recent research improved the understanding of posttranscriptional modifications, which are affecting the fate of a transcript. …
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Investigating nuclear transport proteins as secreted cancer biomarkers
… These results were validated at both mRNA and protein levels, and similar upregulation observed in oesophageal cancer. Recent mass spectrometry (MS) analysis of cancer cell secreted proteins identified elevated levels of 13 members of the nuclear transport protein family in the secretomes …
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The Role of ATP and Cleavage Factor Phosphorylation in pre-mRNA 3' Cleavage Reaction
<p>The 3' end cleavage/polyadenylation (3' processing) is important in generating a functional messenger RNA (mRNA) transcript. It is long-known that ATP can significantly stimulate the in vitro cleavage of adenovirus type 2 L3 (Ad2L3) RNA substrate. Here, we used ATP analogs in structure-activity …