Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"claudin-5"”.
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The Effects of Onion and Yuzu Extracts against Ischemia/Hypoxia-Induced Dysfunction of Blood-Brain Barrier
… of ZO-1 and FoxO3a-mediated degradation of claudin-5 during ischemia/hypoxia using the mouse middle cerebral artery occlusion (MCAO) model and hypoxia model. The possible underlying mechanisms are also investigated, especially those linked to the oxidative stresses. Onion extract (OE) and …
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The effects of ethanol and aspalathus linearis on immortalized mouse brain endothelial cells (bEnd5)
… rate of cell division (iv) cell toxicity (v) claudin-5 transcription (vi) permeability across a monolayer of bEnd5 ECs and (vii) morphology, for a selected experimental timeline of 24, 48, 72, and 96hrs. We then investigated if the simultaneous exposure of Rf and EtOH could reverse or …
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MOLECULAR PHYSIOLOGY OF BLOOD-BRAIN BARRIER TIGHT JUNCTIONS
… aspects of the pores that are feasible under claudin-5 tight junction assembly. We performed a mutation experiment that distinguished the dimeric interfaces between claudin-3 and claudin-5, further a biophysical investigation showed how the flexibility of the transmembrane domains affect the …
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Coregulatory Transcription Factors and Spermatogenesis
… in Etv5 -/- mice, and that this was mediated by Claudin 5 (CLD5). In both Etv5-/- and W/Wnu mice, the BTB was leaky. Examination of CLD5 expression among mice of various ages and phenotypes revealed the following: both ETV5 and germ cells were necessary for seminiferous epithelial CLD5 …
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Organ Specific Vascular Response to Fibrosis Affects Breast Cancer Metastatic Organotropism
… of endothelial tight gap junction protein Claudin-5, the fibrotic kidneys had an elevated expression of Claudin-5.</p> <p>Our findings suggest that despite the similarities between fibrosis, the tumor microenvironment and the pre-metastatic niche, that while it can enhance tropic metastatic …
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Hemodynamic regulation of blood-brain barrier phenotype: A cooperative effort linking adherens and tight junction complexes
… (TJ) proteins (e.g. VE-Cadherin, occludin, ZO-1, claudin-5) jointly consolidate the apical surface of adjacent brain microvascular endothelial cells (BBMvEC’s) creating the blood-brain barrier (BBB), a critical regulator of CNS homeostasis. Junctional disassembly and barrier dysfunction are …
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hiPSC-Derived Cerebral Small Vessel Disease Models
… abnormalities with Occludin discontinuity and Claudin-5 accumulation in hiPSCs-derived Brain Microvascular Endothelial Cells (iBMECs), which resulted in decreased TEER levels and increased permeability. They also induced apoptosis, migration defects, and transcriptome changes in hiPSCs-derived …
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Mechanisms of Hypoxia-Induced Neurovascular Remodeling in PlGF Knockout Mice
… with disruption of the tight-junction protein claudin-5. These vessels displayed large lumens, were surrounded by reactive astrocytes, lacked mural cell coverage and endothelial VEGF expression, and regressed after 21 days of hypoxia. The lack of PlGF, in combination with reduced VEGF …
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Neurotoxic effects of malathion and lead acetate on the blood-brain barrier: Disruptive effects caused by different mechanisms examined with an in vitro blood-brain barrier system
… In general, protein levels of occludin, claudin 5, ZO1, and ZO2 decreased at all times, however, qPCR analysis of gene expression for all the proteins did not correlate with the assessments on protein levels. TRPC channel protein levels increased in response to neurotoxicant insult, which …
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Targeting Organic Anion Transporting Polypeptide 1A4 (Oatp1a4) for Effective Drug Delivery at the Blood-Brain Barrier
… of tight junction proteins (i.e., occludin, claudin-5) or paracellular “leak” of the vascular marker sucrose, indicating that targeting TGF-β/ALK1 signaling does not adversely affect BBB integrity. Overall, our work provides novel evidence that Oatp1a4 transport activity at the BBB is …
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From youth to old age: therapeutic potential of microbiota interventions in the gut-brain-axis
… of prebiotic administration and the level of claudin 5 increased in the hippocampus following consumption of L. mucosae. Overall, these results provide novel insights regarding the impact of a portfolio of microbiota-targeted interventions on age-related alterations in physiology and brain …
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An <i>in vitro</i> human 3D co-culture model to study endothelial-astrocyte interactions
… on hCMEC/D3 cells namely, zonula occludens-l, claudin-5, and P-glycoprotein and similar paracellular permeability coefficients to fluorescent-dextrans (70 kDa).<br></br><br></br>Using the developed model, the effect of endothelial cells on the AQP4 expression in HA was investigated. While the …
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The Effects of Aging on EGFR/pSTAT3-Dependent Gliovascular Structural Plasticity
… proteins such zonula-occludens-1 (ZO1) (6) and Claudin-5 (7). Underlying the execution of these processes is the morphological and spatial arrangement of astrocytes between neurons and endothelial cells comprising blood vessels, where comprehensively speaking, these cells form what is known as …
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MAPK and pro-inflammatory mediators in the walls of brain blood vessels following cerebral ischemia
… the BBB by degrading the tight junction proteins claudin-5 and occludin between the endothelial cells and extracellular matrix molecules constituting the basal lamina surrounding the endothelial cells. When the integrity of the BBB is lost, inflammatory cells and fluid can pass to the brain, …