Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"clathrin-mediated endocytosis"”.
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Regulation of Clathrin Mediated Endocytosis and Its Role in Cancer Progression
… dynamic modulation of cell surface proteins by endocytosis. Given this functional connection, it has been suggested that endocytosis is dysregulated in cancer. To test this, we developed In-Cell ELISA assays to measure three different endocytic pathways: clathrin-mediated endocytosis, …
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Conserved and Novel Properties of Clathrin-Mediated Endocytosis in Dictyostelium Discoideum
<p>The protein clathrin mediates one of the major pathways of endocytosis from the extracellular milieu and plasma membrane. Clathrin functions with a network of interacting accessory proteins, one of which is the adaptor complex AP-2, to co-ordinate vesicle formation. Disruption of genes involved …
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Interrogating regulated intramembrane proteolysis of IGF-1R, and its role in cell migration
… characterised as a substrate for gamma-secretase-mediated regulated intramembrane proteolysis (RIP). This proteolytic pathway begins with ectodomain shedding, mediated by sheddases, which produces a soluble fragment in the extracellular space. Following ectodomain shedding, a membrane anchored …
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Characterisation of Novel Rab5 Effector Proteins in the Endocytic Pathway
Endocytosis, a process of plasma membrane invaginations, is a fundamental cellular mechanism, ensuring uptake of nutrients, enhanced communication between cells, protective functions against invasive pathogens and remodelling of the plasma membrane composition. In turn, endocytic mechanisms are …
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Regulation of Endocytosis of ROMK Channel by WNK Kinase Family
… in the kidney. WNK1 and WNK4 stimulate the clathrin-mediated endocytosis of ROMK, which contributes to the pathogenesis of hyperkalemia in PHAII patients. Intersectin (ITSN) is a multimodular endocytic scaffold protein. The proline-rich domains of WNK1 and WNK4 bind with the Src-homology …
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THE EFFECT OF SKELETAL MUSCLE-SYNTHESIZED BDNF ON MOTOR NEURON SOMA SIZE AND BDNF PROTEIN ABUNDANCE
… (TrkB) at the motor neuron axon terminal causes clathrin mediated endocytosis of the complex and an intracellular signaling cascade that recruits dynein motor proteins for retrograde transport of the BDNF/TrkB endosome to the motor neuron soma. At the motor neuron soma, the BDNF/TrkB complex …
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The 5' inositol phosphatase SHIP2 regulates EGF-elicited protrusion in MTLn3 cells
… membrane-cytoskeleton interactions and clathrin-mediated endocytosis. Here I have looked at the effects of knocking down SHIP2 in the MTLn3 cell line, a metastatic rat breast carcinoma line. I found that when SHIP2 is knocked down in cells, there is an increase in membrane protrusion …
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cpg2 encodes a brain- and synapse-specific protein that regulates the endocytosis of glutamate receptors
… of excitatory synapses, often in the vicinity of clathrin-coated pits. I further show that, through its C-terminal coiled-coil motifs, CPG2 binds to the actin cytoskeleton and to endophilin B2, a member of a family of proteins involved in membrane trafficking. RNAi-mediated knock-down of CPG2 …
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Antibodies to Heterogenous Nuclear Ribonucleoprotein A1 Penetrate Neurons Leading to Multiple Downstream Effects Resulting in Neurodegeneration
… A1 antibodies enter neuronal cells via clathrin-mediated endocytosis. Moreover, we have shown that anti-hnRNP A1 antibodies cause redistribution of endogenous hnRNP A1 protein, decrease in cellular ATP levels, and increase in apoptosis. Additionally, we present data depicting RNA binding …
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Overcoming the plasma membrane barrier to improve the efficiency of therapeutic delivery to the cellular cytoplasm
Difficulties in controlling endocytosis limit the success of many nanoparticle-based drug delivery strategies. Therefore, there is a need to both (i) introduce new mechanisms of therapeutic delivery that overcome the limitations of endocytic uptake, and (ii) gain better control of endocytosis by …
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The Interplay of Signal Transduction and Membrane Traffic of the Macrophage Colony Stimulating Factor Receptor (MCSFR) Controls Macrophage Growth
… we showed that the MCSFR is internalized by clathrin-mediated endocytosis (CME). Coincident with this internalization, the MCSFR stimulates the formation of macropinosomes. These large vacuoles form at the plasma membrane, but are devoid of the receptor. Following these two steps, the Rab5 …
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Studies on the efficient intracellular delivery vehicle using variable region domain of a cell-penetrating anti-DNA monoclonal antibody
… cells and it was mainly internalized by the clathrin-mediated endocytosis pathway. The VH domain did not change the cell cycle and significantly affect the cell proliferation in various mammalian cell lines at the concentration that we used for functional assays. We tested further the …
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The Rac-GEF P-Rex1 Regulates Agonist-Induced GPCR Trafficking
… but also the internalisation of the receptor by clathrin-mediated endocytosis (within minutes) to switch off signalling. Previous data from our lab for the GPCR Sphingosine-1-Phosphate Receptor 1 (S1PR1) suggested a new role of P-Rex in GPCR trafficking. P-Rex overexpression blocks the first step …
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Characterization of the entry mechanisms utilized by the alphavirus venezuelan equine encephalitis virus to infect mosquito cells
… alphaviruses enter mammalian cells via receptor-mediated, clathrin-mediated endocytosis. This endocytic pathway utilizes several proteins in the mammalian cell, including the clathrin protein, the small GTPases known as Rab proteins and the large GTPase dynamin. These proteins have been shown to …
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Cellular determinants of reovirus entry and infection
… techniques to show that these particles can use endocytosis to enter and productively infect host cells. We found that uptake of reovirus virions and ISVPs was inhibited in cells treated with a small molecule inhibitor of dynasore. Analysis of reovirus replication in cells treated with genistein …
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Evading Innate and Adaptive Immunity During Adenovirus Cell Entry
… a non-enveloped, dsDNA virus, enters cells via clathrin-mediated endocytosis. For viral genome delivery to the nucleus, Ad must penetrate endosomal membranes to create defects sufficient for the passage of the 90 nm diameter capsid across cell membranes. Recent observations suggest that …
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Investigating β- and γ-secretase function and processing of APP with genetic and pharmacological tools
… that this effect appears to be independent of clathrin-mediated endocytosis. I conclude that BDNF may reduce production of Aβ by altering BACE1 distribution, decreasing upstream β-cleavage. Lastly, BACE1 and γS play important roles in neural differentiation through regulation of Notch …
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Upconversion Nanoparticles for pH Sensing and Imaging
… the pH of acidic compartments (lysosomes) after clathrin-mediated endocytosis.
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Cationic Glycopolymers for DNA Delivery: Cellular Internalization Mechanisms and Biological Characterization
… route. Pharmacological inhibition of endocytosis and visualization by confocal microscopy reveal that internalization occurs primarily through an actin and dynamin-dependent mechanism. Caveolae/raft-mediated endocytosis appears to be the predominant internalization mechanism, with …
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Polyethylene glycol copolymer nanocarriers: Biocompatibility, uptake and intracellular trafficking in neurons
… effect of nanocarrier size and charge in their clathrin-mediated endocytosis (CME), intracellular trafficking and uptake efficiency in neurons and glia, assessed using immunocytochemistry, confocal microscopy and live cell imaging. We have observed that, irrespective of size and charge, a …
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