Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"class switch recombination (CSR)"”.
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The Role of RNA Helicase RHAU in Immunoglobulin Class Switch Recombination
… isotypes through a process known as class switch recombination (CSR). In these cells, activation induced cytidine deaminase (AID) binds Gquadruplex (G4) switch transcripts, which serve as guide RNAs to target AID to the immunoglobulin heavy chain switch (S) regions in the DNA for CSR. …
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Early divergent B cell differentiation during antibody responses
… follicular or GC responses and the amount of class switch recombination (CSR). The expression of phenotypic markers and switched antibody protein in parallel with gene array analysis and single cell real time RT-PCR in responding B cells was used to identify GC and plasmablast precursors and …
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Investigation of Ring Finger Protein 8 in Mammalian Physiology and Pathogenesis
… such as immunoglobulin heavy chain (IgH) class switch recombination (CSR), an important mechanism for antibody diversification and specification of immunoglobulin effector function during a humoral immune response in mammals. Through catalyzing ubiquitylation of the H2A-type histones …
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Regulation of Immunoglobulin Gene Diversification By Noncoding RNA's
… silencing. These mechanisms range from "classical" RNA interference (RNAi), to gene repression by microRNAs (miRNAs), to maintenance of genomic stability by repeatassociated small RNAs. Here, I describe the contribution of miRNA-mediated regulation to a specific case of gene expression …
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The Role of ZMYND8 in Immunoglobulin Class Switch Recombination
<p>Class switch Recombination (CSR) also known as Immunoglobulin (Ig) Class switching is a genomic recombination/deletion reaction that diversifies the effector component of the antibody response but preserves antigen specificity. CSR is initiated by the enzyme activation induced cytidine deaminase …
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Altered Kinetics of Non-Homologous End Joining Mediated DNA Repair in Mouse Models of Aging and Leukemia
… their bone marrow differentiation and peripheral class switch recombination (CSR), thus lending them as a good model system in which to delineate the DNA repair mechanisms. To determine the effect of aging on NHEJ, B lymphocytes from old mice were analyzed. The results showed compromised DNA …
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The Role of 53BP1 in DNA Double-Strand Break Repair
… the immune cell diversification reactions V(D)J recombination and Class Switch Recombination (CSR) require the formation of DSB intermediates, a process that is tightly controlled and strictly limited to developing B and T cells. CSR in B cells diversifies antibodies by joining DSBs between …
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The Role of Activation-Induced Cytidine Deaminase in Antibody Diversification and B Cell Malignancies
… for somatic hypermutation (SHM) and class switch recombination (CSR) in B cells. SHM alters the affinity of an antibody for its corresponding antigen by introducing point mutations within the variable region of the immunoglobulin (Ig) gene heavy chain locus. CSR replaces the Ig μ …
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The Effect of Activation Induced Cytidine Deaminase Phosphorylation and Herpes Virus Uracil Dna Glycosylase On Antibody Diversification
… of somatic hypermuntation (SHM) and isotype class switch recombination (CSR). AID is targeted to the immunoglobulin gene locus where it deaminates cytosines to generate uracil residues in DNA. This generates guanine-uracil (U:G) mismatch lesion which are recognized by uracil DNA glycosylase …
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Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution
… initiation of somatic hypermutation (SHM), and class switch recombination (CSR), by the C deaminations in immunoglobulin genes. Activity of these enzymes requires scanning of single-stranded (ss) DNA substrates to locate deamination motifs and stochastically deaminate them to create mutational …
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B cell memory in STAT3 Hyper-IgE Syndrome: Cytokine integration, IgE regulation, and the architecture of human memory B cell subsets
… IL-4 and IL-21 in the regulation of IgE class-switch recombination (CSR). We furthermore show that naïve B cells from most IEI, including STAT3 HIES, do not exhibit an intrinsic propensity for IgE CSR in vitro. Aim 2 (Chapter 4) addressed whether IgE+ B cells exist in humans, and found …
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Genome-Wide Characterization of the Effects of Nucleic Acid Modifying Enzymes: Cytidine Deaminases and DNA Methylation
… introduced into immunoglobulin (Ig) genes, and class-switch recombination (CSR), in which genomic constant regions are recombined to encode antibodies of different isotypes. Both of these processes require AID-catalyzed C-to-U lesions at the Ig loci, which are resolved to generate point …
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Atm-/-Ung-/- Mice Present Reduced Levels of Switched Immunoglobulin Isotypes IgG1 and IgG3
… the immunoglobulin heavy chain gene locus during class switch recombination (CSR). CSR is mediated by activation induced cytidine deaminase (AID) and results in a new immunoglobulin isotype that B cells will secrete and present. AID produces mutations that convert cytosines to uracils within the …
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The role of DEAD-box helicase 1 in humoral immune responses
… Primary diversification occurs through VDJ recombination during B-cell development, whereas secondary diversification occurs during ongoing immune responses through the introduction of affinity enhancing point mutations in Ig variable genes, a process termed Somatic Hypermutation (SHM). …
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The modulation of autoimmune disease progression in mouse models
… that regulates somatic hypermutation (SHM) and class switch recombination (CSR), the two key mechanisms in affinity maturation. We showed that genetic ablation of AID significantly inhibited the development of TID in NOD mice. Homologous recombination (HR) pathway is important for the repair of …
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Taming a Potent Mutator: Identification and Characterization of Novel Mechanisms of Regulating Antibody Diversification in B-Lymphocytes
… such processes: Somatic Hypermutation (SHM) and Class Switch Recombination (CSR). AID-dependent deamination of cytidine bases within the variable region (SHM) and switch region (CSR) of the immunoglobulin locus (Ig) results in the modification of the antigen binding domain and diversity within …
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Post Translational Regulation of AID Targeting to Both Strands of a Transcribed DNA Substrate
… affinity by participating in two reactions, class switch recombination (CSR) and somatic hypermutation (SHM). Both reactions occur after VDJ recombination, subsequent to antigen exposure. During CSR, a deletion and recombination event occur to alter the effector function from IgM to either …