Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"chromosome conformation"”.
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Unfolding genome organization in interphase
… frequency maps obtained from high throughput chromosome conformation capture technologies have revealed several organizing patterns of mammalian interphase chromosomes, including self-interacting topologically associating domains (TADs) which are believed to function as coherent gene …
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Conformation and dynamics of the mammalian chromosome
… towards this goal. We characterize the ensemble chromosome conformation in mouse embryonic stem cells, by measuring interaction, or contact, probabilities between distal genomic loci. We map and describe chromosome loops, consisting of two interacting CTCF sites co-bound by cohesin, that maintain …
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Genome organization by loop extrusion
… propagate in the same way - they duplicate their chromosomes and divide into two daughter cells, each containing one copy of each chromosome. Because of the extreme length of chromosomes, their two copies have be compacted in order to be successfully segregated from each other. The process of …
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UNCOVERING STRUCTURE-FUNCTION RELATIONSHIPS IN CHROMATIN ARCHITECTURE
… and their inner subTADs in high resolution Chromosome-Conformation-Capture Hi-C data. We then apply our method to chromatin dynamics during mitosis, uncovering how TADs and subTADs evolve temporally. Afterwards, we classify TADs/subTADs by the presence of other genome folding features, …
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Dynamics of Genome Organization
… over the past 15 years, following the advent of chromosome conformation capture techniques. The fourth dimension---time---however, has remained elusive and the dynamics underlying the organization of the genome are much less known. In this thesis I present my contributions to our understanding of …
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Genomic Regulation of the Shox2 Gene during Mouse Limb Development
… signature profiling, we have utilized circular chromosome conformation capture (4C-seq) technology to probe the entire cis-regulatory organization of the Shox2 gene during limb development. We show five of numerous genomic regions within a 1.5 megabase (Mb) chromosomal territory surrounding the …
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Linking genome organization to nuclear function in astrocytes and muscle cells
… interactions captured by HiC and Circular Chromosome Conformation Capture (4C) for mito-nDNA interactions in order to determine if they are random. I show that the mito-nDNA interactions captured in mammalian cells are statistically significant and shared between biological and technical …
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Identificazione e analisi funzionale di fattori regolatori dei geni globinici
… variants at these loci we applied “Genome Wide Chromosome Conformation Capture” (Hi-C), followed by a novel technique for a selective enrichment at these target regions, to characterize whether they are involved in long range physical interactions able to modulate HBS1L-MYB and BCL11A …
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Patterns and processes driving chromosome organization
… analysis of interaction maps generated from Chromosome Conformation Capture (3C) technologies. We begin by analyzing data generated using a novel Micro-C protocol and assess its performance in comparison to established Hi-C. New computation tools are developed to extract, quantify and compare …
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Microfluidic technology for cellular analysis and molecular biotechnology
… valves and mixers for sensitive study of chromosome conformation is presented. This device has small reaction chamber minimizing sample requirement, and allows multiple steps of biological analysis in a single chip avoiding sample loss during sample transfer. Several orders of magnitude …
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Computational Analysis of Transcriptional Regulation
… for early cervical carcinogenesis with a novel Chromosome Conformation Capture (3C) method that specifically enriches for interactions involving viral integrants. Integration occurs without disrupting host 3D chromatin structure but alters the expression of many neighbouring host genes. The …
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Linker Histone Medicated Regulation of Mitotic Chromosome Compaction and Individualization
<p>Mitotic chromosomes are scaled to the cell size to ensure effective chromosome segregation. Recent studies have shown how condensins and DNA topoisomerase II organize the mitotic chromosome. However, the regulation of these factors in maintaining proper chromosome size in different cell types …
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Spatial organisation of the immunoglobulin heavy chain locus and inter-chromosomal gene networks driving B cell development
… of the Igh locus facilitate V(D)J recombination. Chromosome conformation capture techniques have revolutionised studies of genome conformation. I have applied a novel form of enriched Hi-C to study both intra-locus (cis) and genome-wide (trans) interactions of the immunoglobulin loci in pro-B and …
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Integrative analysis of transcriptional activity and genome architecture changes upon viral infections
… viral infections, I employed a genome-wide chromosome conformation capture approach (Hi-C) on infected murine and human cells and further enriched those libraries for genomic loci of interest and the viral genomes with biotinylated RNA baits. In parallel, I profiled newly transcribed RNA …
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An Investigation Into The Role Of Cfia 3' End Processing Complex In The Termination And Initiation/reinitiation Of Transcription
… absence of a functional Clp1 protein. Using the chromosome conformation capture approach, CCC, we observed a role for Clp1 in gene loop formation. We found a strong correlation between the Clp1 function in gene looping, and its role in promoter-associated transcription which implies gene looping …
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The Nucleosome Remodelling & Deacetylase complex: Genome folding & transcriptional regulation
… gene regulation. At the very largest scales, chromosomes are known to occupy distinct chromosome territories, intermingling only at the borders. At the very smallest scale, chromatin is known to be organised into a beads on a string like structure of nucleosomes. Until the advent of Chromosome …
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The interplay of global chromosomal organisation, promoter-enhancer interactions and transcription
… control. In recent years, the emergence of chromosome conformation capture and related methodologies has dramatically increased our understanding of chromosomal organisation. In particular, high-throughput Hi-C analyses across cell types have led to the identification of spatial genomic …
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Mechanisms of epigenetic regulation in epidermal keratinocytes during skin development. Role of p63 transcription factor in the establishment of lineage-specific gene expression programs in keratinocytes via regulation of nuclear envelope-associated genes and Polycomb chromatin remodelling factors.
… role in establishing distinct three-dimensional conformations in the Epidermal Differentiation Complex (EDC) locus (Fessing et al., 2011). Here we show that in p63-null mice about 32% of keratinocytes showed altered nuclear morphology. Alterations in the nuclear shape were accompanied by …
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Higher Order Chromosome Organization and Recombination Dynamics of Meiotic Prophase I In Mouse Spermatocytes
<p>Meiotic recombination is required for parental chromosomes to find each other (pairing/synapsis) and to exchange genetic information thus allowing faithful segregation of chromosomes and the production of haploid gametes. At the start of meiotic prophase I, meiotic chromosomes organize into loop …
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