Global ETD Search
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Showing 1 to 9 of 9 for “"chromogenic substrate"”.
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Development of Field-deployable Nucleic Acid Testing Platforms
… based on measuring the absorbance change of a chromogenic substrate which is triggered by DNA and DNA intercalating dyes under light illumination. The performance of both devices was tested using synthetic DNA, nucleic acid amplicons, and actual parasites nucleic acid samples collected from …
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Choline Inhibition of Prothrombinase
… II and Va, phospholipid, Ca<sup>2+</sup>, and chromogenic substrate (Kabi S-2238). The release of p-nitroaniline from S-2238 was measured, by continuous spectrophotometric monitoring of incubation mixtures at 400 nm, as an index of IIa production.</p> <p>Under these assay conditions, choline …
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NOVEL CINNAMIC ACID-BASED DEHYDROPOLYMERS FOR EMPHYSEMA: IN VITRO AND IN VIVO ASSESSMENT OF THEIR ACTIVITIES
… DHP. These activities were determined by chromogenic free radical generation in chemical oxidation, lung epithelial (Calu-3) repression of pro-inflammatory nuclear factor κB (NFκB) upon its plasmid transfection and chromogenic substrate NE hydrolysis, respectively. The sulfated caffeic …
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Development and evaluation of a colorimetric coliphage assay detection system
… infection. The released enzyme then cleaves a chromogenic substrate which produces a colored reaction product. The CCADS was evaluated against the APHA method under laboratory conditions using a common sewage coliphage strain as a model (American Type Culture Collection- 13706-B2), and under …
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Anti-platelet aggregation activity of melaleuca bracteata var.revolution gold derived betulinic acid and its derivatives
… to separately inhibit the hydrolysis of chromogenic substrate was used for antithrombin activity of the triterpenoids. The release of ATP and calcium mobilization from the cytosol, as platelets aggregate, was investigated using a commercial kit and Fura 2/AM respectively. The …
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Antithrombotic and anti-inflammatory activity of ethyl acetate extract of Protorhus longifolia stem bark
… ion chelating potential were also determined. A chromogenic substrate was used to determine the antithrombin activity of the extract. While the thrombolytic activity of the extract was determined in rat whole blood clot, the antiplatelet aggregation activity was evaluated on rat platelet rich …
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Thrombin-Inhibiting Perfluorocarbon Nanoparticles: A New Class of Therapeutic for Acute Thrombosis Treatment and Diagnosis
… evaluated inhibition of thrombin cleavage of the chromogenic substrate Chromozym TH and defined the kinetics of the particle-thrombin interaction. Thrombin-inhibiting activity of the component inhibitors was undiminished on the nanoparticles and, as explored in appended work, the nanoparticles had …
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Modulating metalloproteinase activity with protein nanofibrils
… > 70 % for MMP2 and 9) was confirmed using a chromogenic substrate. The selectivity for MMPs observed here could potentially be due to the differences between their catalytic subsites (S1) pocket. These preliminary results demonstrate the potential of WPNFs to modify MMP activity, …
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Mechanisms of polyphosphate degradation
… terminal phosphates with alcohols, we created chromogenic and fluorogenic polyphosphatase substrates that allow one to measure real-time activity of either endo- or exopolyphosphatases, depending upon assay configuration. We confirmed the products' identities through 1D and 2D 31P, 1H, and 13C …