Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"chromatin regulation"”.
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Chromatin Regulation in Somatic Cell Reprogramming to Pluripotency
Contains fulltext : 215040.pdf (Publisher’s version ) (Open Access)
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Dissecting the Architectural Properties of Chromatin and the Influence of the H1 Linker Histone on Chromatin Regulation
… this research, I used an existing coarse-grained chromatin model from the Collepardo Lab to study the binding of the H1 linker histone to chromatin and delve into the intrinsic properties of chromatin that regulate its architecture. The primary aim is to decipher the factors modulating chromatin …
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Characterizing the Nuclear Function of the Cell Adhesion Molecule Fat and Chromatin Regulation by the Inner Nuclear Membrane Protein Speg
… nucleus. In the nucleus, Ft is associated with chromatin. Using DamID-microarray, a DNA binding site mapping technique, I showed that Ft binds to specific genomic loci in vivo. Through ChIP-seq, I characterized the genome-wide DNA binding profile of Ft in vivo. ChIP-seq results suggested that …
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Regulation and function of histone variant H2BE in neurons
… of four histone proteins: H2A, H2B, H3, and H4. Regulation of histone proteins affects gene expression through multiple mechanisms, including exchange with histone variants. While variants of H2A and H3 have well defined roles in chromatin regulation, widely expressed variants of H2B remain …
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Investigating the Role of the Lysine-Specific Demethylase 4C in Pancreatic Ductal Adenocarcinoma
<p>Deregulation of proteins involved in chromatin regulation is common in pancreatic ductal adenocarcinoma (PDAC). Lysine demethylase 4C (KDM4C) is one of the chromatin modifying proteins frequently overexpressed across multiple solid cancers and is linked to chromatin instability, increased cell …
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The dissection of VEGFA stimulus-responsive regulatory and transcriptional changes in angiogenesis
… and transcription factor binding profiles in regulation of angiogenesis, the formation of new blood vessels. First, I identify regulatory elements involved in VEGFA-response through focal, temporal changes in chromatin structure and that p300 activity is mechanistically required for this …
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A role for the histone variant HTZ1 in meiotic chromosome segregation
… cells, the packaging of genomic DNA into chromatin has important consequences for all DNA-dependent transactions. Chromatin structure is highly regulated by a variety of complex processes that are not well understood. These include nucleosome remodeling and post-translational modification …
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H2A.Z : a molecular rheostat for gene regulation in embryonic stem cells
Chromatin regulation is a key mechanism for controlling gene expression patterns during development and differentiation. The histone H2A variant H2A.Z is highly conserved among eukaryotes and is of particular interest because it has an essential, yet unknown role in early development. H2A.Z is …
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RNA Dysregulation in a Novel Human Model of TDP43 Proteinopathy
… dendritic defects, apoptosis, microRNA dysregulation, as well as hyperphosphorylation and, likely, aggregation of TDP43. Simultaneously, the nuclear loss of TDP43 results in widespread alternative splicing errors. In a doxycycline-inducible version of this model, we demonstrate that …
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The Analysis of the MCF7 Cancer Model System and the Effects of 5-AZA-2'-Deoxycytidine Treatment on the Chromantin State Using a Novel Microarray-Based Technology for High Resolution Global Chromatin State Measurement
A microarray method to measure the global chromatin state of the human genome was developed in order to provide a novel view of gene regulation. The 'chromatin array' employs traditional methods of chromatin isolation, microarray technology, and advanced data analysis, and was applied to a cancer …
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Core Components Of The Nuclear Pore Bind Unique States Of Chromatin And Mediate Polycomb Repression
… that comprise the NPC, are also involved in chromatin-related processes such as the regulation of genes and chromatin architecture. However, whether individual Nups bind and regulate distinct types of chromatin remains poorly understood. To decipher how Nups are involved in different aspects …
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Evolutionary, structural and functional features of cellular signalling networks
… histories suggested a conserved origin in chromatin regulation, followed by functional diversification. Chapter 4 extends the scope to signalling via transcriptional regulation, and presents a comprehensive overview of the interactome of the stem cell transcription factor Oct4. The results …
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Insights into the regulation of the CRL4D̳T̳L̳ ubiquitin ligase
… DNA repair processes, transcription, and chromatin regulation. CRL4DTL_ mediated targeting is restricted to S phase and after DNA damage through a PCNA-dependent mechanism. Recent studies have focused on elucidating the requirements within the substrates that dictate CRL4DTL-mediated …
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Cellular and genetic mechanisms of new tissue production in the regenerating planarian Schmidtea mediterranea
… of RNAi, the genetic basis for stem cell regulation in Schmidtea mediterranea has remained largely unexplored. Using microarrays, a genome-scale approach was taken to identify and characterize genetic factors specifically expressed in proliferating cells (neoblasts) of adult planarians. …
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STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… small non-coding RNAs in RNA metabolism and gene regulation, the mechanisms by which RNA polymerase III (Pol III) is selectively recruited to snRNA promoters remain poorly understood. This is particularly notable because Pol II- and Pol III-transcribed snRNA promoters share highly similar …
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A SEARCH FOR EPIGENETIC VULNERABILITIES OF AML CELLS PERFORMED BY A CDK INHIBITOR¿BASED CRISPR SCREENING
… of hematopoietic progenitors. Epigenetic dysregulation plays a central role in AML pathogenesis, and targeting chromatin-modifying enzymes has emerged as a promising therapeutic strategy. Among these, the histone demethylase LSD1/KDM1A has been extensively studied, yet clinical responses to …
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Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… have implications for nuclear organization and chromatin regulation. Cholesterol is an abundant sterol in the membranes of higher eukaryotes that is important in mammalian physiology and disease. This ancient lipid serves multiple essential functions in membranes and signaling, but the precise …
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Mechanisms of piRNA-guided co-transcriptional gene silencing
… and replicate within a host genome. Heterochromatin establishment at these sequences prevents ectopic recombination and TE mobilisation. In *Drosophila* ovaries, this depends on Piwi, which is guided by a bound small RNA to nascent TE transcripts where it regulates the formation of …
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Biophysical Analysis of S100A12 and the Nucleosome Core Particle
<p>Calcium signaling and chromatin remodeling are fundamental regulatory processes that control protein activity and gene expression, respectively. This thesis investigates the molecular mechanisms by which local ligand binding and structural perturbations propagate allosteric effects across larger …
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Exploring the role of endogenous DNA damage in neurodegeneration
… from a variety of sources, including metabolism, chromatin remodelling, transcription, and replication stress. Defective DNA repair machinery leads to various human diseases. Underlying these diseases is – at a basic level – the inability to counteract specific types of genotoxic stressors. The …
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