Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"chromatin organisation"”.
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Role of DNA replication timing in gene expression and chromatin organisation
… are associated with altered transcription and chromatin organisation, it is still unknown whether DNA replication timing (RT) is a cause or a consequence of cellular fate changes. During my thesis I used a conditional system to perturb DNA RT in a single cell cycle in budding yeast, in …
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Study of the role of plant nuclear envelope and lamina-like components in nuclear and chromatin organisation using 3D imaging
… morphology and meiosis, its implication for chromatin organisation has been less studied in plants. The fi aim of this work was to develop NucleusJ a simple and user-friendly ImageJ plugin dedicated to the characterisation of nuclear morphol- ogy and chromatin organisation in 3D. NucleusJ …
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Chromatin Dynamics During DNA Replication
The primary level of chromatin organisation consists of arrays of nucleosomes that are present across the genetic template. Advances in the post genomics era have made it possible to determine the positions of nucleosomes genome-wide where it has been observed that nucleosomes adopt a distinct …
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Characterisation of Nuclear EnvelopeAssociated Proteins (NEAP) in Arabidopsis thaliana
… and protecting the genome, which is organized in chromatin, a structure wrapping DNA around histone proteins. The NE is composed of two membranes: on the nucleoplasmic side, the Inner Nuclear Membrane (INM) and on the cytoplasmic side, the Outer Nuclear Membrane. The NE allows communication …
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Super-resolution Imaging of Chromatin and Functional Nuclear Architecture
… understood to be linked to the three-dimensional organisation of the genome within the nucleus, however, the mechanisms through which the different levels of organisation regulate genes are poorly understood. Newly developing super-resolution imaging techniques offer an important new way to …
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Computational Analysis of Transcriptional Regulation
… features of this are the three-dimensional (3D) chromatin organisation of eukaryotic genomes and the post-transcriptional control of RNA fates. Eukaryotic nuclear DNA is tightly packaged as chromatin that is further folded into higher order structures. The 3D folding of eukaryotic genome lends …
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Dissecting the Architectural Properties of Chromatin and the Influence of the H1 Linker Histone on Chromatin Regulation
… this research, I used an existing coarse-grained chromatin model from the Collepardo Lab to study the binding of the H1 linker histone to chromatin and delve into the intrinsic properties of chromatin that regulate its architecture. The primary aim is to decipher the factors modulating chromatin …
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The Role of Maelstrom in the Drosophila piRNA Pathway
… regulated through the establishment of heterochromatin which prevents TE mobilisation. Piwi guided by piRNAs to nascent TE transcripts, it recruits factors necessary for creating transcriptionally repressive chromatin. Maelstrom, a protein that is involved in transposon silencing within the …
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The role of Arabidopsis thaliana heterochromatin component H2A.W in meiotic recombination
… heterochromatic regions. Heterochromatin is compacted with a high DNA density and is enriched in nucleosome occupancy and specific chromatin modifications, including H3K9me2 and DNA methylation. Analysis of histone H2A variants via ChIP-sequencing has revealed that H2A.W is …
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Die epigenetische Regulation des C 4 -Syndroms : belichtungsabhängige Chromatinveränderungen am Promotor der Phosphoenolpyruvat Carboxylase aus Mais (Zea mays L.)
… is inhibited. We analyzed alterations in the chromatin structure on the promoter of the C4 specific isoform of phosphoenolpyruvate carboxylase (PEPC) after illumination of seedlings. A protocol was established that facilitates the preparation of nuclei from maize leaves with intact chromatin …
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A multidisciplinary investigation into the function of NFXL1
… CG15011-binding sites enriched for cytoskeletal organisation and neuronal projection morphogenesis. Weighted gene co-expression network analyses (WGCNA) in human, mouse, and Drosophila datasets revealed conserved roles in transcriptional regulation, RNA processing, and protein modification. Cell …
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Single-molecule G-quadruplex visualisation in live cells and 3D chromatin
… the potential involvement of G4s in 3D chromatin organisation. For this, G4 imaging was extended into 3D by super-resolution microscopy in fixed cells, and combined with single-cell Hi-C method. Hi-C is a fixed nucleus chromatin conformation capture technique that can simulate 3D …
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The Nucleosome Remodelling & Deacetylase complex: Genome folding & transcriptional regulation
… is highly dependent on the three dimensional organisation of chromatin within a cell’s nucleus. Approximately 2 metres of DNA is folded to fit within a nucleus which is on the order of a few micrometers. In addition to simply fitting within the space available, this folding directly influences …
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The epigenetics and role of Dopa Decarboxylase in heart development
… in controlling gene expression by regulating chromatin organisation, and has been shown to be fundamental for imprinting of the Igf2/H19 locus through its binding to the germline differentially methylated region (gDMR). This thesis explores the occurrence of CTCF binding both genome-wide and …
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Transcription Factor Binding Dynamics and Spatial Co-localization In Human Genome
… however, the relationship between genome spatial organisation and transcription factor binding is not well studied. Using the recently available high resolution Hi-C contact map of human GM12878 lymphoblastoid cells, we investigated computationally the genome-wide spatial co-localization of …
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Regulatory architecture of the Pax6 locus
… studies. I have set out to understand how the chromatin architecture of the locus contributes to the mechanism and specificity of cis-regulatory interactions. As well as addressing whether the DNA looping model for regulatory interactions applies to the mouse Pax6 locus, I will identify which …
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Interrogating novel functions of the I kappa B kinases via CRISPR-Cas9 gene editing and small molecule inhibition
… expression of a broad range of genes involved in chromatin organisation, cytoskeletal organisation, mitotic cell cycle control and the DNA damage response. During the characterisation of IKK KO cells it was discovered that the expression of NEMO was downregulated at the protein, but not mRNA level …
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The chromatin landscape of colorectal cancer cells
Chromatin organization is at the heart of deciphering gene regulation as it is instructive to transcription. Current technological advances in next-generation sequencing approaches have offered unprecedented opportunities to interrogate the genomic landscape in multiple pathological and clinical …
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Transcriptomic and proteomic dysregulation in neuropsychiatric disorders
… immunity, translation, complement cascade and chromatin organisation. Furthermore, theoretically motivated gene lists associated with MDD (e.g., cell cycle genes) were evaluated within each sex, and sex-specific dysregulations in replication-dependent histones, ribosomes, OXPHOS - electron …