Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 204 for “"chromatin immunoprecipitation"”.
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Reproducibility and reliability of chromatin immunoprecipitation in clinical research
… and epigenetic silencing. Changes to chromatin structure influence gene expression via histone modifications. Chromatin immunoprecipitation (ChIP) is an experimental technique used to investigate the interactions between histones and DNA. ChIP determines the specific location in the …
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The developmental function of Pax7 : chromatin-immunoprecipitation discovery of Pax7 target genes
Pax7 plays critical roles in development of brain, spinal cord, neural crest and skeletal muscle. As a sequence-specific DNA-binding transcription factor, the direct functional role played by Pax7 during development is the selection of target genes. To date, an accurate description of the function …
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Motifs, binding, and expression : computational studies of transcriptional regulation
… techniques like transcriptional profiling and chromatin immunoprecipitation have emerged and taken on increasing importance in the study of regulatory processes. Mining these experiments for useful biological information requires methods that can handle large quantities of noisy data and …
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Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production
… with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of …
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Conformation and dynamics of the mammalian chromosome
… of transcriptional mechanisms, their impact on chromatin structure, and concomitant functional consequences. Employing chromatin immunoprecipitation measurements, we report progress towards this goal. We characterize the ensemble chromosome conformation in mouse embryonic stem cells, by …
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Microfluidic Engineering for Ultrasensitive Molecular Analysis of cells
… and fast molecular analysis of cells. Chromatin immunoprecipitation (ChIP) assay followed by next generation sequencing serves as the primary technique to characterize the genomic locations associated with histone modifications. However, conventional ChIP-seq assay requires large …
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Analysis Of The Role Of Amphetamine In The Induction Of Changes In Histone Modifications Of Dopaminergic Pathway Genes
… modifications. For, gene specific investigation, chromatin immunoprecipitation was employed. Chromatin immunoprecipitation revealed that amphetamine treatment down regulates the expression of dat-1, the dopamine transporter and cat-1, vesicular monoamine transporter by reducing the enrichment of …
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The Sumoylation of General Transcription Factors Regulates Transcription in Saccharomyces cerevisiae
… or reduced TFIID or TFIIF sumoylation. Using chromatin immunoprecipitation (ChIP), we found that GTF sumoylation does not substantially impact GTF-chromatin interactions but can impact the promoter-wide sumoylation level. Notably, TFIID and TFIIF sumoylation enhances RNAPII recruitment at …
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The Novel Regulation of Histone Modification In Cancer Development
… H3 acetylation. Genome-wide analysis of chromatin immunoprecipitation followed by sequencing (ChIP-seq) data sets reveals that NEDD4 regulates glucose-induced H3K9 acetylation at transcription starting site (TSS) and enhancer regions. Integrative analysis of ChIP-seq and microarray data …
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Recording Transcription Factor Binding During Development
… named "Calling Cards", offers an alternative to chromatin immunoprecipitation (ChIP) methods and furthermore has the ability to record transcription factor binding through cell division and development. I first demonstrated the method using the Ty5 transposon in the budding yeast Saccharomyces …
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Practical Approaches to Biological Network Discovery
… how network inference results can complement chromatin immunoprecipitation: ChIP) experiments, which directly probe the binding events of transcriptional regulators. Collectively, my contributions improve both the accessibility and practicality of network inference methods.
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Zero-Inflated Models to Identify Transcription Factor Binding Sites in ChIP-seq Experiments
… controls gene regulation in living organisms. Chromatin immunoprecipitation followed by highthroughput sequencing (ChIP-seq) is considered the gold standard in locating these binding sites and programs use to identify DNA-transcription factor binding sites are known as peak-callers. ChIP-seq …
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Communication between layers in biological transcriptional networks
Chromatin-immunoprecipitation experiments in combination with microarrays (known as ChIP-chip) have recently allowed biologists to map where proteins bind in the yeast genome. The combinatorial binding of different proteins at or near a gene controls the transcription (copying) of a gene and the …
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Control of Neuroendocrine Cell Physiology by a Single Transcription Factor, Drosophila Basic Helix Loop Helix Regulator DIMMED
… factor. To accomplish this, I use in vivo chromatin immunoprecipitation: ChIP) followed by measuring DIMM binding to putative DIMM enhancers by quantitative Polymerase Chain Reaction: qPCR). This work is described in Chapter 2. Secondly, I extend DIMM ChIP analysis to identify direct DIMM …
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P107 negatively regulates the neural precursor pool by repressing Hes1 transcription
… subsequently found to bind E2F3 and E2F4 using chromatin immunoprecipitation. P107 was found to significantly repress Hes1 promoter activity in the luciferase reporter assay, and finally, using the primary neurosphere assay we showed that removal of Hes1 from p107-/- neurospheres restores the …
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Epigenetic analysis of promiscuous gene expression in central tolerance
… the regulation of transcription. Through native chromatin immunoprecipitation on thymic epithelial cells transfected with AIRE, we show how in vitro, TRA are enriched in active acetylation and methylation of core histones, yet retain silencing modifications. Furthermore, across a cluster of …
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Regulation of flagellin glycosylation genes in Campylobacter jejuni: influence of NssR, the nitrosative stress response regulator.
… of the affected genes was deduced and chromatin immunoprecipitation (ChIP) used to map NssR binding to six potential locations in the region. Methods to measure bacterial motility were assessed and used to document the defective motility of the nssR mutant, which was found to be due to …
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Characterization of the Ets-family transcription factor Ets98B in Drosophila
… control properties, it may be worthwhile to use chromatin immunoprecipitation to identify target genes, as well as perform structure-function analysis to map the transactivation domain(s).
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Microfluidic Technology for Low-Input Epigenomic Analysis
… pluripotency/differentiation and tumorigenesis. Chromatin immunoprecipitation (ChIP) is the technique of choice for examining in vivo DNA-protein interactions and has been a great tool for studying epigenetic mechanisms. However, conventional ChIP assays require millions of cells for tests and …
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Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response
… host DNA via its tandem repeat domain. Utilizing chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) with E. chaffeensis-infected cells, TRP47 was found to bind at multiple sites in the human genome (n = 2,051 at p < 10-30). Ontology analysis identified genes …
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