Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"chromatin dynamics"”.
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Chromatin Dynamics During DNA Replication
The primary level of chromatin organisation consists of arrays of nucleosomes that are present across the genetic template. Advances in the post genomics era have made it possible to determine the positions of nucleosomes genome-wide where it has been observed that nucleosomes adopt a distinct …
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Investigating the chromatin dynamics of gene activation
… this model into question. Understanding the dynamics of enhancers and promoters during transcriptional activation is vital for comprehending how gene expression is regulated by enhancers. Advances in techniques enabling the labelling and tracking of non-repetitive loci in live cells have …
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Transcription and chromatin dynamics in the Notch signalling response
… by cell signalling pathways and the pre-existing chromatin environment. The highly-conserved Notch signalling pathway is involved in many cell fate decisions during development, activating different target genes in different contexts. Upon ligand binding, the Notch receptor itself is cleaved, …
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Modelling chromatin dynamics in neural stem cell quiescence and reactivation
… level remains lacking. Previous work on the chromatin state of quiescent stem cells in different systems showed variable results, from globally increased heterochromatin to subtle changes in enhancer activation. An increased understanding of the role of chromatin in neural stem cell dynamics …
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A study of chromatin dynamics during transcription by fluorescence light microscopy
… exist as DNA-RNA-protein complexes known as chromatin. The structure of chromatin fluctuates to allow controlled access to genetic information while maintaining its important packaging function. Recent improvements in optics, image acquisition electronics, and live imaging techniques, as well …
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Investigating the chromatin dynamics that underlie CRISPRi-validated enhancer-promoter interactions
… mass detection of genome wide epigenetic marks, chromatin conformation, chromatin accessibility, and expression data. Nevertheless, the process of validating enhancer targets remained costly until recent developments of CRISPRi methods enabled large scale perturbation experiments for identifying …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… and in vivo. However, the specific mechanisms of chromatin modulation by NuRD in this process have not been fully characterised. This study used rapid, auxin-inducible removal of NuRD components in mouse ESCs to dissect its role in the regulation of transcription factor (TF) binding dynamics, …
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CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY
… (EMS) can regulate nuclear morphology and chromatin dynamics via the physical link from focal adhesions to chromatin via the cytoskeleton, linker proteins on the nuclear envelope and the nuclear lamina. However, how such force transmission from cytoskeleton to the nucleus is regulated by …
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Rigidity in Frictional Particle Packings and Dynamics in Living Polymeric Packings
… of the cell nucleus. The cell nucleus houses chromatin, which is linked to a protein shell called the lamina. Protein motors and chromatin binding proteins in the nucleus are thought to drive correlated chromatin dynamics and nuclear shape fluctuations. To test this notion, we develop a …
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UNCOVERING STRUCTURE-FUNCTION RELATIONSHIPS IN CHROMATIN ARCHITECTURE
… enabled the creation of high-resolution maps of chromatin architecture genome-wide from which to test new biological hypotheses. In this thesis, we first present a networks based algorithm, 3DNetMod, for identifying the prominent feature of Topological Associating Domains (TADs) and their inner …
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An Investigation of Epigenetic Mechanisms Driving The Biology of Head and Neck Squamous Cell Carcinoma
… the role of enhancer landscapes and overall chromatin dynamics have recently been recognized as important regulators of cancer cell activity and tumor microenvironment (TME) interactions. In the case of HNSCC, these facets of epigenetic regulation are understudied and present a critical …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
Epigenetic reprogramming and chromatin structure guide differentiation and define cell identity during embryogenesis. To maintain cell identity and genome integrity, epigenetic marks are faithfully inherited during DNA replication through tightly coordinated processes. Our recent work demonstrated …
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Epigenetic Modification as a Therapeutic Target in BRAFV600E-mutated Metastatic Colorectal Cancer
… of the studies lies in revealing abnormal chromatin dynamics of BRAF<sup>V600E</sup> CRC and the determination of optimal epigenetic therapeutic intervention in BRAF<sup>V600E</sup> CRC patients. Based on the preclinical data, the combination of BET, BRAF, and EGFR inhibition will be …
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Regulatory network built by Cytochrome c with Histone Chaperones in response to DNA damage
… assist the DNA repair mechanisms altering chromatin dynamics through their nucleosome assembly activity. Recent studies have reported that the mitochondrial respiratory protein cytochrome c is promptly translocated into the cell nucleus upon DNA damage, where it interacts with several …
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Postmitotic Dynamics in Chromatin Modification and Regulatory Topology Underlie Cerebellar Granule Maturation
… niche. This is mediated at least in part by dynamics in chromatin biology, as mutations in chromatin regulators are strongly implicated in the advent of neurodevelopmental and neuropsychiatric disorders. The mechanisms by which chromatin dynamics orchestrate neuronal maturation remain poorly …
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TRANSCRIPTIONAL NETWORKS VARIATIONS DURING CELL CYCLE PROGRESSION IN HUMAN EMBRYONIC STEM CELLS
… signalling pathways with the epigenetic state, chromatin structure and transcriptional networks during cell cycle progression and these mechanisms remain to be fully uncovered. Here, I have utilised the FUCCI reporter system combined with ATAC-Seq to analyse chromatin dynamics during cell cycle …
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Deciphering genome-wide chromatin occupancy, dynamics, and their connections to gene regulation
… DNA is bound by a myriad of proteins to form the chromatin inside the nucleus of the cell. The proteins can bind to the genome in different combinations leading to a combinatorial explosion in the number of possible chromatin configurations. The differences in the chromatin configurations for the …
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The interaction of promoter chromatin architecture with the cell cycle regulates transcription activation kinetics
… metabolism and differentiation. In eukaryotes, chromatin dynamics on gene promoter sequences is an integral part of regulation, and nucleosome remodeling is often required for transcription activation. Though the transient response of these regulated genes is often important in biological …
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Mechanisms and functional significance of nuclear compartmentalization
… at velocities of 1-2 μm min-1. Observation of chromatin stretching in the direction of movement demonstrated a force generating mechanism. Transcription in nearly all cases increased noticeably only after initial contact with a nuclear speckle. The time lag between initial speckle contact and …
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Role of Chromatin Structure and JmjC Histone Demethylases in the Response to Hypoxia
… programmes for survival and adaption. How the chromatin state coordinates these changes remains largely unknown. Global histone methylation changes occur in response to hypoxia, however, temporal dynamics of histone methylation changes and how they correlate with hypoxia induced gene …
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