Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 33 for “"chromatin compaction"”.
-
Genomic and microscopic dissection of large-scale chromatin compaction
Large-scale chromatin compaction varies across the human genome, and these variations correlate with differences in transcriptional activity in a limited number of model systems. However, without validated genome-wide methods directly measuring large-scale chromatin compaction, the degree to which …
-
An in vivo study of chromatin compaction in human cells
Chromatin was one of the first sub-cellular structures to be described in early microscopy studies (Heitz 1928). Chromatin is formed from DNA, RNA, histones and other associated proteins. Within the nucleus, chromatin is arranged as compacted heterochromatin and decompacted euchromatin. During cell …
-
Analyzing a calcium-dependent UV response that results in a global chromatin compaction in mammalian cells
… However, the structural change in the global chromatin in response to UV radiation and the etiology of these changes are not well known. To visualize a global chromatin structural changes upon UVB radiation, we used a fluorometric detection approach. We observed large-scale chromatin …
-
Effects Of Dna Methylation Pattern On The Chromatin Structure
… DNA methylation levels have been associated with chromatin compaction leading to gene silencing. For example, abnormal DNA methylation is associated with silencing of tumor suppressor genes and is observed in the onset of tumorigenesis. There is evidence suggesting that not all methylation events …
-
Regulation of polycomb repressive complexes at the neural differentiation gene Pax6
… underlying this phenomenon is the regulation of chromatin structure around neural differentiation genes.<br/><br/>The analysis of chromatin structure by fluorescence in situ hybridisation uncovered that chromatin compaction at the neural differentiation gene Pax6 is maintained by FGF signalling …
-
Regulation of Histone Covalent Modifications During Yeast Apoptosis
<p>Chromatin compaction is a hallmark property of apoptosis, a highly coordinated suicide mechanism generally believed to be confined to vertebrates. However, invertebrates such as the budding yeast, Saccharomyces cerevisiae, display an apoptotic-like phenotypes including chromatin condensation, …
-
A study of chromatin dynamics during transcription by fluorescence light microscopy
… exist as DNA-RNA-protein complexes known as chromatin. The structure of chromatin fluctuates to allow controlled access to genetic information while maintaining its important packaging function. Recent improvements in optics, image acquisition electronics, and live imaging techniques, as well …
-
Investigating the interactions of G-quadruplexes with transcription and chromatin structure
… the relationship between the folding of G4s and chromatin accessibility at promoters. The dependence of promoter G4 formation on transcription was tested following Inhibition of transcription elongation and initiation by 5,6-dichlorobenzimidazole 1-ß-D-ribofuranoside and triptolide respectively. …
-
H4K16 acetylation during embryonic stem cell differentiation
… in that it has been directly associated with chromatin decompaction. H4K16ac has been linked to development through its Histone Acetyltransferase, MOF. Deletion of MOF in mice leads to mass chromatin defects, and embryonic lethality prior to the blastocyst stage. I set out to understand the …
-
Reexamining the role of linker histones beyond 30 nm fibers in a complex chromatin environment
… store DNA in the cell nucleus in the form of chromatin. Chromatin is composed of nearly equal parts proteins and DNA. It is both highly compacted and organized into discrete domains within the nucleus. However, the manner in which chromatin is compacted, and domains are organized, remains …
-
Role of Phosphorylated Dicer1 In Tumor Progression
… that the murine AT2 tumor cells exhibit altered chromatin compaction. Specifically, I find that the presence of phosphorylated nuclear DICER1 in the AT2 tumor cells leads to “open” chromatin at loci of gastrointestinal genes and enhancer regulatory elements which are normally “closed” in …
-
The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase
… exhibited a significant increase in binding to chromatin during stationary phase. Because of the role of H1 in gene expression and chromatin compaction, it is essential to understand the mechanism behind this change in binding behaviour for a complete mechanistic description of gene regulation. …
-
Gene expression from unintegrated HIV-1 is restricted by the SMC5/6 complex
… Unintegrated viral genomes are rapidly chromatinised by the host cell, forming the basis for epigenetic regulation. I showed that the SMC5/6 complex binds unintegrated viral genomes in an SLF2-dependent manner, suggesting that the complex acts directly on viral chromatin. Concomitantly, …
-
Creating a versatile toolkit for transgene expression using BACS and for dissecting large-scale chromatin organization
… highly compacted with histone proteins to form chromatin in interphase nuclei. Both the higher levels of chromatin folding and the spatial organization of the chromatin, referred to as large-scale chromatin organization, have been shown to correlate with transcriptional activity. One example …
-
Development And Application Of Next Generation Sequencing Pipelines In Arabidopsis
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a widely used assay to uncover the function of proteins of interest by generating a snapshot of the protein’s binding sites in an organism at a certain stage given specific conditions. Previous publications provide recommendations …
-
Time Resolved Light Sheet Microscopy
… show the capability of this system to measure chromatin compaction in three dimensions.<br/><br/>However, fluorescence lifetime imaging has some drawbacks, acquisition times on such a system can range from the tens of seconds to minutes, which is often too long to comprehensively measure many …
-
Characterisation of HP1γ in mammalian cells
The degree of chromatin compaction plays a fundamental role in controlling the accessibility of DNA to the transcription machinery as well as other DNA-dependent biological pathways. The mammalian HP1 (Heterochromatin protein 1) protein family consists of three members: HP1α, β and γ. Each …
-
Advancing fluorescence lifetime microscopy for quantitative bioimaging
… a new sensing technique was developed to study chromatin compaction in living cells, based on the fluorescence lifetime of a widely used, far-red, DNA-binding dye. The technique was validated in multiple cell types, tissues, and stem cells. Unlike existing methods (optical or otherwise), our …
-
A New Type of Programmed Cell Death in C. Elegans
… stereotypical morphological features including chromatin compaction and by a requirement for the activity of caspase proteases, which are controlled by conserved gene pathways. Although non-apoptotic, caspase-independent programmed cell death pathways have been postulated, there is little …
-
The role of the chromatin remodeller ISWI in the cellular response to hypoxia
… access sequences in DNA that are integrated in chromatin. It is known that the chromatin remodelling complex SWI/SNF (Switch/Sucrose non-fermenting) is essential for HIF activity. However, no additional information exists about the role of other chromatin remodelling enzymes. This thesis …
Page 1 of 2