Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"chromatin binding"”.
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Structural studies of paired PHD finger-bromodomain chromatin-binding modules: targeting epigenetic readers with chemical probes
… in this thesis aims to target reader domains of chromatin-associated proteins with small molecules, in order to provide information on their ligandability, useful to develop - potent chemical probes. This thesis work is divided in three parts.<br/><br/>In the first and second part it is shown how …
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Genetic analysis of novel regulators of neuronal migration in Caenorhabditis elegans: the insulin/IGF-1 signaling pathway, a chromatin-binding factor ZFP-1 (AF10) and endogenous RNAi
The generation of functional neural circuitries requires neuronal migration, a central component of proper nervous system development. When defective, it can lead to devastating conditions including epilepsy and mental retardation. In the nematode C. elegans, neurons undergo both short- and …
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An integrated network of estrogen receptors alpha and beta, and coregulators, for deciphering estrogen signaling in breast cancer cells
… their comparative abilities to localize at chromatin binding sites across the genome, and the recruitment of major coregulators such as SRC3 and RIP140 by the ERs, and the association of ERs with other transcription factors and chromatin remodeling factors is incompletely understood. …
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Proteomic characterisation of Estrogen Receptor (ERα) interactome in breast cancer
… between ERα and various factors in regulating chromatin binding and gene transcription, underlying estrogen signalling in breast cancer. During the last decade, quantitative mass spectrometry (MS)-based proteomics has become a powerful tool for the study of protein interactions. However, …
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Novel plant nuclear envelope-associated coiled-coil proteins
… proteins called lamins, as well as lamin- and/or chromatin-binding inner nuclear membrane (INM) proteins. The components of the metazoan lamina lack sequence homologues in plants. There is however evidence of a network of nuclear filamentous proteins underlying the NE. This study aims to …
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Investigating upstream regulators of FOXA1 in breast cancer
… to the nucleus, where it interacts with chromatin-associated factors and modulates FOXA1 interactions with SWI/SNF complex members and other nuclear cofactors. PIGS depletion also impacts chromatin binding of both FOXA1 and ERα. To identify factors influencing FOXA1 protein levels, we …
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Exploiting Lentiviral Integrations to Profile Chromatin
… modifications and protein localisation along chromatin suffer from critical limitations: the gold standard, ChIP-seq, relies on high-quality antibodies, while other antibody-free techniques like DamID are limited by the availability of specific DNA sequences. Here, I describe the development …
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Core Components Of The Nuclear Pore Bind Unique States Of Chromatin And Mediate Polycomb Repression
… that comprise the NPC, are also involved in chromatin-related processes such as the regulation of genes and chromatin architecture. However, whether individual Nups bind and regulate distinct types of chromatin remains poorly understood. To decipher how Nups are involved in different aspects …
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Discovery of Novel Ubiquitin- and Methylation-Dependent Interactions Using Protein Domain Microarrays
… known H2Bub1-specific readers. Using a ubiquitin-binding domain microarray, I discovered the phospholipase A2-activating protein (PLAA) PFU domain as a novel H2Bub1-specific reader. PFU domain interacts with H2Bub1 in the context of histone acid extracts but not recombinant nucleosomes, suggesting …
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Transcriptional effects of predator cues in the brain of Spodoptera frugiperda (lepidoptera: noctuidae) moths exposed to bat ultrasound
… metabolism, actin cytoskeleton dynamics, chromatin binding and other epigenetic modifications, axonal guidance and remodeling, cilia function and development, Wnt signaling, and TOR signaling. The top five significantly over-represented GO terms included chromatin binding, macromolecular …
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Functionality of mutant p53 in early tumorigenesis
… molecular mechanism using the latest -omics and chromatin binding assays. Despite the DN activity being strong and p53R175H-dose-dependent in acute stress, the cells show a stress-dependent cell fitness response, with the chronic stress state making the heterozygous p53R175H less fit than …
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Single-molecule microscopy reveals details of heterochromatin reorganisation at the onset of mouse ES cell differentiation
… we have come a long way in describing how chromatin folds – from the level of single nucleosomes all the way to compartments and chromosomal territories – it is still unclear what determines this structure and why it changes upon differentiation. Mouse embryonic stem cells provide an …
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The physiological role of the two isoforms of DNA topoisomerase II in human cells
… topoisomerase IIbeta diffused away from the chromatin despite the nuclear lack of the IIalpha-form. Chromosome condensation and disjunction was performed with the aid of cytosolic topoisomerase IIalpha which bound to the mitotic chromatin with low affinity. Consequently an increased rate of …
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Plant Homeodomain Finger Protein 20 (Phf20) and Its Homolog Phf20 Like 1 (Phf20L1) Define Two Distinct Non-Specific Lethal (Nsl) Complexes
… enrichment sites of PHF20 and PHF20L1 on chromatin were also identified by chromatin-precipitation (ChIP) followed by next-generation sequencing (ChIP-seq) and mapped to the promoters of genes that were highly expressed in cells. Results from ChIP followed by quantitative RT-PCR …
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Control of Chromosome Segregation by the Aurora B Complex
… requires spindle assembly around M-phase chromatin. In cells lacking centrosomes, such as those found in female meiosis, chromosomes themselves nucleate and stabilize microtubules in order to promote accurate spindle formation. Here we present a description of the composition and function …
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Regulation of Chromatin Multi-Scale Organization and Function by Linker Histone H1
… ranging from single basepair modifications to chromatin compartments spanning many megabasepairs, and innumerable factors influence and regulate genome structure and function. One factor that appears to play a key role is linker histone H1, which is the most prevalent chromatin-binding protein …
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Single-Molecule Investigation of Chromatin-Associated Factors in Genome Organization and Epigenetic Maintenance
… gene regulation lies in the complex structure of chromatin. This scaffold of structural proteins and enzymatic regulators determines what genes are expressed at what times, leading to cell differentiation, cell fate, and often disease. Currently, the field of chromatin biology has relied on basic …
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ELUCIDATING THE MOLECULAR DETERMINANTS OF P53-MEDIATED PLEIOTROPIC EFFECTS IN SMALL CELL LUNG CANCER
… p53 transcriptional output without impacting p53 chromatin binding. This work demonstrates that a previously unappreciated intertumoral heterogeneity in SCLC can influence the biological response to p53 restoration, describes a novel mechanism of p53-regulated cell death, and uncovers putative …
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RNA localizations of proteins that interact with the chromosome passenger complex
In the last twenty years work on chromatin binding non-coding RNAs has revealed much of their biology, but how mitosis affects these nuclear ncRNA transcripts is less well understood. This study examined the behavior of several ncRNAs through mitosis. It is understood that Aurora B Kinase, or …
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