Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"chromatin architecture"”.
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UNCOVERING STRUCTURE-FUNCTION RELATIONSHIPS IN CHROMATIN ARCHITECTURE
… enabled the creation of high-resolution maps of chromatin architecture genome-wide from which to test new biological hypotheses. In this thesis, we first present a networks based algorithm, 3DNetMod, for identifying the prominent feature of Topological Associating Domains (TADs) and their inner …
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Chromatin Architecture Aberrations Contribute and Acute Lymphoblastic Leukemia Relapse
… These aberrations can be found within the chromatin architecture of cancer cells that includes the genome, molecular modifications to the genome, and the three-dimensional organization of the chromatin fiber. The majority of genetic variants target non-coding regions of the genome and many …
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Roles of the Histone Chaperone FACT in Drosophila Gene Regulation and Chromatin Architecture
… conserved histone chaperone FACT (Facilitates Chromatin Transcription) is thought to contribute to the disassembly and reassembly of nucleosomes in the wake of RNA polymerase II passage through chromatin. In yeast, mutation of FACT subunits leads to decreased nucleosome occupancy at the …
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The interaction of promoter chromatin architecture with the cell cycle regulates transcription activation kinetics
… metabolism and differentiation. In eukaryotes, chromatin dynamics on gene promoter sequences is an integral part of regulation, and nucleosome remodeling is often required for transcription activation. Though the transient response of these regulated genes is often important in biological …
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DNA topology and Pol II CTD phosphorylation as stepwise regulators of chromatin architecture in differentiating human stem cells
… guided by enhancers, excluded from heterochromatin, and repositioned through DNA looping. While each of these features of Pol II regulation has been well studied in individual cell types, it remains unclear how a single genome can support hundreds of distinct fates. Combinatorial …
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Regulatory Landscapes Associated With Changes In Nuclear Architecture
… gene regulation is played by the cell’s chromatin architecture. Gene positioning relative to the center of the nucleus or its three-dimensional contacts with other genomic regions can determine whether the gene is expressed or not. Changes in chromatin architecture have been reported in …
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Engineering Cell Lines to Investigate the Function of Mammalian SWI/SNF Complexes
… SWI/SNF complexes belong to the class of chromatin remodelling complexes, and consist of approximately 15 subunits. Bioinformatics analyses of tumour sample sequencing studies have shown that members of the mSWI/SNF complexes are mutated in almost 20% of all cases, suggesting many subunits …
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Dissecting the Architectural Properties of Chromatin and the Influence of the H1 Linker Histone on Chromatin Regulation
… this research, I used an existing coarse-grained chromatin model from the Collepardo Lab to study the binding of the H1 linker histone to chromatin and delve into the intrinsic properties of chromatin that regulate its architecture. The primary aim is to decipher the factors modulating chromatin …
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Directed evolution in human cells via adenoviral replication
… cellular trafficking, and highly regulated chromatin architecture can all derail the activity of BOIs evolved in simpler systems. Current approaches to directed evolution in metazoan cells rely on labor-intensive and time-consuming screening approaches that have a high potential for false …
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Mechanism of Atrial Fibrillation in Lamin A/C Cardiomyopathy
… by which a rare pathogenic LMNA variant disrupts chromatin architecture, leading to the perturbation of atrial gene regulatory networks that involve regulatory regions harboring AF-associated variants and transcription factors crucial for atrial rhythm control and contractility. Using CRISPR …
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Role of ORCA and ORC in chromatin organization and DNA replication
… or individual subunits of ORC including heterochromatin organization, telomere and centromere function, centrosome duplication and cytokinesis. Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases …
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Regulatory architecture of the Pax6 locus
… studies. I have set out to understand how the chromatin architecture of the locus contributes to the mechanism and specificity of cis-regulatory interactions. As well as addressing whether the DNA looping model for regulatory interactions applies to the mouse Pax6 locus, I will identify which …
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Investigating 3D genome organisation in Caenorhabditis elegans with Accessible Region Conformation Capture (ARC-C)
… have been applied in various organisms to study chromatin architecture. However, these methods have limitations: most of them are limited to restriction-fragment resolution and all of them, with the exception of Hi-C, only survey a pre-defined subset of the genome. I developed a variant of Hi-C, …
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Super-resolution Microscopy: Novel Developments and Optimisations
… discussed in the thesis, is the study of chromatin architecture in primary spermatocytes of Drosophila. This includes optimisation of imaging conditions and data analysis software to reconstruct features with different densities of labelling dye in the imaged nuclei. Calibration and …
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Changes of nucleosome positioning and 3D chromatin organization in cell transitions
… the nucleus in a highly condensed form called chromatin. The basic unit of chromatin is the nucleosome. The positioning of nucleosomes on the DNA determines the accessibility of transcription factors (TFs) and other regulatory molecules. Beyond nucleosome positioning, the higher level of 3D …
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Uncovering the Roles of Human C2H2-Zinc Finger Protein ZNF121 in Gene Regulation
… an elegant arrangement of protein machinery and chromatin architecture, and the resulting pre-mRNA is post-transcriptionally processed and regulated at every step to buffer, reduce, or amplify its protein output. Chemical nucleotide modifications on DNA or RNA are one type of gene regulatory …
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The genomic anchors of the human major histocompatibility complex
Eukaryotic chromatin is organised into a hierarchy of topologically constrained loop structures. Matrix Attachment Regions (MARs) are genomic sequences that mediate the anchoring of chromatin to the insoluble proteinaceous fraction of the nucleus known as the nuclear matrix. Since only a few MARs …
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Role of chromatin condensates in tuning nuclear mechano-sensing in Kabuki Syndrome
… forces that shape nuclear morphology and architecture. The balancing between two chromatin compartments with antagonist functions, namely Transcriptional and Polycomb condensates, is required for preserving nuclear mechanical properties and its perturbation is causative of the pathogenic …
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Core Components Of The Nuclear Pore Bind Unique States Of Chromatin And Mediate Polycomb Repression
… that comprise the NPC, are also involved in chromatin-related processes such as the regulation of genes and chromatin architecture. However, whether individual Nups bind and regulate distinct types of chromatin remains poorly understood. To decipher how Nups are involved in different aspects …
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