Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"chondroitin sulfate"”.
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Regulation of Bacteroides chondroitin sulfate utilization genes
… I have completed the first such study using the chondroitin sulfate utilization (csu) system in Bacteroides thetaiotaomicron as the model system to study regulation.
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CHEMICAL SYNTHESIS AND BIOLOGICAL EVALUATION OF CHONDROITIN SULFATE DISACCHARIDES
… units. Recently, it was shown that the sulfate groups present in chondroitin sulfate GAGs (CS) encode important functional information for the regulation of physiological processes. However, procurement of pure, homogenous CS motifs for biological evaluation is a challenging task due to …
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Transport studies of chondroitin sulfate disaccharide through articular cartilage
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1997.
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Glycosyl Transferases in Chondroitin Sulfate Biosynthesis: Subcellular Location and Elongation Reaction
… transferase involved in the synthesis of chondroitin sulfate were examined in cultured embryonic chick tibial chondrocytes. The chondrocytes were subjected to subcellular fractionation by differential centrifugation and sucrose density gradient centrifugation. The glucuronosyl transferase, …
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Outer Membrane Proteins Associated With Utilization of Chondroitin Sulfate by Bacteroides Thetaiotaomicron
… a cell-associated inducible system to degrade chondroitin sulfate (CS), a mucopolysaccharide that may be a natural carbon source for this organism in the colon. The system that V. thetaiotaomicron uses to break down CS is not fully understood. Chondroitin lyase, an enzyme which cleaves CS into …
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Genetic Analysis of Chondroitin Sulfate Utilization in Bacteroides Thetaiotaomicron (Cloning, Mutagenesis, Polysaccharide)
… of the genes involved in the utilization of chondroitin sulfate, a complex polysaccharide which is probably a source of carbon and energy for the bacteria in the human colon. B. thetaiotaomicron produces two inducible chondroitin lyases (I and II) when it is grown on chondroitin sulfate. I …
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Enzymatic and analytical tools for the characterization of chondroitin sulfate and dermatan sulfate glycosaminoglycans
… important subset of GAGs, namely heparin/heparan sulfate-like glycosaminoglycans (HSGAGs). The study of chondroitin sulfate and dermatan sulfate (CS/DS), an equally important subset of GAGs, has lagged behind partially due to a lack of enzymatic and analytical tools akin to those developed for …
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In vitro detection of cues that guide thalamocortical development : a role for chondroitin sulfate proteoglycans
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Brain and Cognitive Sciences, 1996.
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Cartilage mechanobiology : the effects of loading on the fine structure and function of chondroitin sulfate glycosaminoglycans
Chondroitin sulfate is a critical component of articular cartilage due to its contribution to the tissue's resistance to compressive deformation. Alterations in the biosynthesis of this molecule over time could impact the ability of the tissue to perform its necessary functions. Several factors …
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HPLC and mass spectrometry analysis of the free radical degradation of chondroitin sulfate and its implications in osteoarthritis
Chondroitin sulfate (CS) is vital component of bone and cartilage; it is widely used as a daily supplement in the management of arthritis. Reactive oxygen species (ROS) are involved in vast array of biological processes ranging from regulatory functions to damaging effects in disease pathogenesis. …
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Inverse Monte Carlo simulation of biomolecular conformation and coarse-grained molecular modeling of chondroitin sulfate conformation, titration, and osmotic pressure
The first part of this thesis is concerned with the solution structure determination problem. Whereas many biomacromolecules, such as proteins, can be adequately characterized by a single conformation in solution, numerous other important molecules (e.g., nucleic acids, carbohydrates, and …
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Regulation and function of hyaluronan binding by CD44 in the immune system
… not been well characterized. Here I identify chondroitin sulfate addition to CD44 as a negative regulator of hyaluronan binding. Chondroitin sulfate addition was analyzed by sulfate incorporation and Western blotting and determined to occur at serine 180 in human CD44 using site-directed …
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An Analysis of the Collagen Synthesized by Chondrocyte Cultures Derived From the Chick Embryo Tibiotarsus
… distinct zones of the tibiotarsus synthesize chondroitin sulfate and type II collagen in primary and secondary cultures. The collagen is secreted into two extracellular collagen pools, the medium (CM) and matrix (Ma) fractions. The CM-collagen fraction contains procollagen and the …
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Crystallographic Studies of Cation-Glucuronic Acid Interactions: Structures of Calcium-Bromide Glucuronate and Sodium Glucuronate.
… of sodium, potassium, and calcium cations to chondroitin sulfate polyanions.
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Studies on the Asparagine-Linked Oligosaccharides From Cartilage-Specific Proteoglycan (Chondrocytes)
… several types of carbohydrate chains, including chondroitin sulfate, keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies.
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VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS
… involved in the developing joint. Versican is chondroitin sulfate proteoglycan. It has four functional-modules: the N-terminal domain, the C-terminal domain, and GAG-[alpha], and GAG-[beta] chondroitin sulfate attachment regions. The N-terminal domain is also known as the G1 domain and the …
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The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
… was used to study the distribution of sulfated disaccharide units along chondroitin sulfate chains. The transglycosylation activity of the hyaluronidase was eliminated by use of excess levels of a second enzyme, (beta)-glucuronidase to remove all nonreducing terminal D-glucuronic acid …
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Delivery of large molecules via microneedles and self-gelating biodegradable hydrogels for the treatment of degenerative bone conditions
… taken by patients with osteoarthritis include chondroitin sulfate and glucosamine sulfate, both of which are classed as nutraceuticals rather than pharmaceuticals, and as such, are not subject to the stringent regulations surrounding pharmaceutical products. Rather, they are required to meet …
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