Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 69 for “"chondrogenic"”.
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Chondrogenic capacities of equine synovial progenitor populations
… cells were maintained in pellet culture with chondrogenic medium for 20 days. Expression of chondrocytic phenotypes was assessed by quantitative RT-PCR of chondrocyte-specific genes, DMMB assays for deposition of sulphated glycosaminoglycans (sGAG), an ELISA assay for collagen type II (Coll …
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Optimizing chondrogenic factors and protein delivery methods for cartilage repair
… The hypothesis that the addition of Dex to chondrogenic medium would affect matrix production and aggrecanase activity of human and bovine BMSCs in RAD and agarose hydrogels was tested. The effects of Dex were dependent on the hydrogel material and the species/age of the BMSCs. Importantly, …
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Stimulations and modulations of chondrogenic differentiation of human embryonic stem cells
… micromass systems, BMP2 was shown to induce chondrogenic differentiation and maturation/hypertrophy, of which distinct temporal pattern differences were observed. Further analysis suggested that BMP2 probably exerts a bipotent effect in inducing both chondrogenic and osteogenic …
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3D Bioprinting of High Cell Density Bioink for Chondrogenic Differentiation in Replacement Tracheal Rings
Tracheal stenosis represents a complex medical challenge due to its debilitating effects on respiratory function. This condition is characterised by the narrowing or obstruction of the tracheal lumen, often resulting from trauma, prolonged intubation, infection, or congenital abnormalities. Severe …
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The Effects of Low Intensity Ultrasound on Chondrogenic Differentiation of Rabbit Mesenchymal Stem Cells In Vivo
… (LIUS), investigated the effects of LIUS on chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs). The hypothesis is that LIUS may be a non-invasively effective stimulant to a biological system in vivo by turning on differentiation of MSCs and promotion of …
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Modulation of Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells through Signals in the Extracellular Microenvironment
… matrix composite scaffolds to guide chondrogenic and osteogenic differentiation. In an osteochondral defect environment, interactions exist between bone marrow cell populations. Although MSCs have been extensively utilized for their ability to support hematopoietic stem and progenitor …
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Osteogenic and chondrogenic differentiation of rat bone marrow stromal cells in 3D culture with novel PCL-TCP scaffolds
… to investigate the efficacy of osteogenic and chondrogenic differentiation of rat bone marrow stromal cells (BMSC) in 3D PCL-TCP scaffolds. PCL-TCP scaffolds fabricated by fused deposition modeling (FDM), are honeycombed, highly reproducible bioresorbable 3D structures with a completely …
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Fibroblast growth factor and its role in the chondrogenesis of canine mesenchymal stromal cells in three-dimensional collagen scaffolds
… bFGF enhances both the mitogenic and chondrogenic potential of human mesenchymal stromal cells (hMSCs). The effect of bFGF on canine mesenchymal stromal cells (cMSCs) during chondrogenesis remains to be determined. The aim of the present study was to determine the temporal effect of …
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Bone growth following demineralized bone matrix implantation requires angiogenesis
… to define the role of angiogenesis during the chondrogenic phase of endochondral bone formation. Day 2, day 8, and day 16 post-surgery were selected time points to represent pre-chondrogenic, chondrogenic, and bone mineralization stages, respectively. Plain x-ray and micro-CT analysis showed …
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CONNECTIVE TISSUE GROWTH FACTOR IS REQUIRED FOR CHONDROGENESIS
… paralleling induction of known early chondrogenic markers (sox9, sox6, l-sox5, type II collagen, aggrecan, decorin and link protein). Real-Time PCR, Western blot and Immunofluorescence reveal that CCN2 is required for the early stages of chondrogenesis, including expression of sox6 and …
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Functional Tissue Engineering of Cartilage Using Adipose-derived Stem Cells
… ASCs have demonstrated significant potential for chondrogenic differentiation. Though studies have demonstrated the ability of ASCs to synthesize cartilage-specific macromolecules, a more thorough understanding of factors that modulate ASC chondrogenesis is required. Accordingly, the central aim …
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Elucidating the Relationship Between Macrophages and Synovial Mesenchymal Progenitor Cell Chondrogenesis
… into cartilage but for unknown reasons, sMPC chondrogenic capacity is lost in OA. This thesis elucidates the relationship between macrophages and sMPC using synovial explants obtained from OA and normal patients. I found that altered macrophage activity (through cytokine stimulation and/or …
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Stem cell matrix and cartilage regeneration
… their synergistic effects. Although the chondrogenic index was comparable between DECM expansion and FGF-2 supplementation, which were much higher than expansion on plastic flask alone, the observations that FGF-2 induced hypertrophic marker genes suggested the superiority of DECM in …
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Decellularized cartilage as a chondroinductive material for cartilage tissue engineering
… in stem cells by quantifying gene expression of chondrogenic markers. The results demonstrate for the first time that DCC can indeed upregulate chondrogenic markers and may be a new chondroinductive material that can provide microenvironmental cues and signaling to promote stem cell …
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Development of a High-Throughput Human iPSC Chondrogenesis Platform and Applications for Arthritis Disease Modeling
… chondroprogenitors demonstrated an improved chondrogenic capacity compared to unselected populations, improved matrix homogeneity, and reduced variability between tissues. We next demonstrated the ability of the system to serve as a high-throughput system for arthritis disease modeling using …
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Self-assembling peptide hydrogels promote in vitro chondrogenesis of bone marrow-derived stromal cells : effects of peptide sequence, cell donor age, and method of growth factor delivery
… cells (BMSCs) and incorporating chondrogenic cues to stimulate differentiation and neotissue production. To test the hypothesis that self-assembling peptide hydrogels provide cues which enhance the chondrogenic differentiation of BMSCs, a technique for rapid, high-viability BMSC …
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Neural and Physical Niches Orchestrate Skeletal Remodelling and Promote Cartilage Regeneration
… a major barrier for translational therapy. With chondrogenic differentiation, hBMSCs/SSCs undergo hypertrophy <i>in vitro</i> and mineralisation <i>in vivo</i>, leading to inferior fibrocartilage and bone formation. However, hBMSCs/SSCs attached to a fibrin microbead scaffold coated with …
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3D printing of hyaluronic acid scaffolds for tissue engineering applications
… on HAGM scaffolds with the aim of inducing chondrogenic differentiation. Cartilage formation on 3D printed scaffolds is better distributed within the scaffolds relative to 2D controls, and new tissue is best able to infiltrate their microperiodic structure when a cell-adhesive RGD peptide is …
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Preconditioning Methods in Cartilage Tissue Engineering: Influences of Silk Material Properties and Hypoxia on Chondrogenesis
… regulator during native cartilage development. Chondrogenic differentiation was induced in human embryonic stem cells under hypoxic conditions, in conjunction with biochemical cues from bovine chondrocytes. As a result, SOX9, a key transcription factor of cartilaginous lineage, was upregulated …
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