Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"chondrogenesis"”.
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Chondrocyte biomechanics and chondrogenesis
This thesis contributes to two aspects of cartilage research: understanding the biomechanical characteristics of single chondrocytes, and tissue engineering musculoskeletal cartilages with human embryonic stem cells (hESCs). Both of these areas of investigation are motivated by the significant …
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Delineating the molecular mechanisms regulating chondrogenesis
… been shown to play a critical role throughout chondrogenesis. Haploinsufficiency of Sox9 in humans leads to a skeletal malformation syndrome known as campomelic dysplasia. To understand the regulation of Sox9 during chondrogenesis, the developing mouse limb was used to identify and characterize …
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CONNECTIVE TISSUE GROWTH FACTOR IS REQUIRED FOR CHONDROGENESIS
… is not known. We've used in vitro models of chondrogenesis to show that CCN2 expression is induced during chondrogenesis, paralleling induction of known early chondrogenic markers (sox9, sox6, l-sox5, type II collagen, aggrecan, decorin and link protein). Real-Time PCR, Western blot and …
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Enhancing Mesenchymal Stem Cell Chondrogenesis for Cartilage Tissue Engineering
… mesenchymal stem cells (MSCs), which can undergo chondrogenesis, have emerged as a promising cell source. To date however, the functional properties of MSC-based constructs remain lower than those of the native tissue and of chondrocyte-based constructs cultured identically. Therefore, the overall …
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A MULTISCALE APPROACH TO STEM CELL-BASED CHONDROGENESIS FOR CARTILAGE REPAIR
… with microscale architecture for improved chondrogenesis and function. Stable channels of varying microscale dimensions were formed in collagen-based and polydimethylsiloxane membranes via a combination of microfabrication and soft-lithography. Human mesenchymal stem cells (hMSCs) were …
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Elucidating the Relationship Between Macrophages and Synovial Mesenchymal Progenitor Cell Chondrogenesis
Osteoarthritis (OA) is a complex disease affecting articular cartilage and joint tissues. Macrophages in OA synovium contribute to the inflamed joint environment by releasing inflammatory mediators promoting cartilage loss. What remains unknown is how macrophages interact with synovial mesenchymal …
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Effects of cytoskeletal protein disruption on the deformation of MSCs during chondrogenesis
Intervertebral disc degeneration has been identified as a main cause for low back pain, a large health problem in the Western world today with 60%-80% of people affected at some point during their lives. Current treatments alleviate the symptoms rather than repair the tissue, but tissue engineering …
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Chondrogenesis of Stem/Progenitor Cells by Chemotaxis Using Novel Cell Homing Systems
… bioactive scaffolds for cell recruitment and chondrogenesis. This thesis develops novel systems to characterize stem/progenitor cell migration and uses the knowledge gained from these systems to develop new methods for inducing chondrogenesis by cytotactic homing. First, the concept of …
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Development of a High-Throughput Human iPSC Chondrogenesis Platform and Applications for Arthritis Disease Modeling
… the generation of a high-throughput system for chondrogenesis and its application for screens and arthritis disease modeling. Future applications of this platform may be useful for identifying pathways regulating cartilage regeneration and novel therapeutics for arthritis.</p>
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Preconditioning Methods in Cartilage Tissue Engineering: Influences of Silk Material Properties and Hypoxia on Chondrogenesis
Cartilage has limited intrinsic healing potential, due to the low cell density and the lack of blood supply. Current treatments for cartilage repair rarely restore full structure and function to the native state. Tissue engineering holds promise to create cartilage grafts capable to withstand the …
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Influence of rearrangement of actin cytoskeleton on the overall material properties of ATDC5 cells during chondrogenesis
… prechodrocytic cells were embedded in agarose, chondrogenesis was triggered in these cells. This work attempts to understand the mechanical implications of this behavior. It also provides an estimate of the sensitivity of the apparatus that would be required to carry out compression loading …
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Self-assembling peptide hydrogels promote in vitro chondrogenesis of bone marrow-derived stromal cells : effects of peptide sequence, cell donor age, and method of growth factor delivery
… all three hydrogels underwent TGF-3 1-mediated chondrogenesis as demonstrated by comparable gene expression and ECM biosynthesis. Cell proliferation occurred only in the peptide hydrogels, not in agarose, resulting in higher sulfated-glycosaminoglycan content and more spatially uniform …
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Synthesis and characterization of matrix metalloproteinase sensitive hydrogels for articular cartilage engineering
… and evaluates the constructs ability to induce chondrogenesis of encapsulated marrow stromal cells for cartilage tissue engineering applications. The scaffold was formed from an unsaturated macromer, oligo(poly(ethylene glycol) fumarate), developed in our laboratory. A matrix metalloproteinase …
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Regulation of chondroprogenitor cell gene expression and migration
… expression changes during osteoarthritis and chondrogenesis is key in understanding how the disease develops and how cartilage might attempt to repair itself. In this thesis, an enhanced model of chondrogenesis of the murine ATDC5 chondroprogenitor cell line was developed with cells cultured …
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Stimulations and modulations of chondrogenic differentiation of human embryonic stem cells
… aims to understand the full span process of chondrogenesis, and how this process could be regulated by growth factors BMP2 and/or TGFI?1 in a controlled context both in vitro and in vivo. By culturing hESCs in 2-D EB monolayer and 3-D high-density micromass systems, BMP2 was shown to induce …
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Cell-seeded type II collagen scaffolds for articular cartilage tissue engineering
… density and bioreactor culture environment on chondrogenesis in cell-seeded type II collagen scaffolds in vitro, and to begin to test effects of implant compositional maturity (viz. glycosaminoglycan, GAG, content) on chondral defect repair. Scaffold cross-link density, a determinant of …
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Identifying sm22 as a key player in arterial diseases
… atherosclerosis where inflammation and osteochondrogenesis are present. However, the role of this downregulation in arterial pathogenesis is unknown. Hypothesis : Downregulation of SM22 may actively contribute to arterial pathogenesis. Methods : Five Sm22 knockout (Sm22<sup>-/-</sup>) mice …
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Functional Tissue Engineering of Cartilage Using Adipose-derived Stem Cells
… understanding of factors that modulate ASC chondrogenesis is required. Accordingly, the central aim of this dissertation was to study the chondrogenic response of ASCs to biochemical, biomechanical, and biomaterial factors.</p><p>We hypothesized that factors, other than TGF-beta and …
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Chitosan fibrous scaffolds for cartilage tissue engineering
… structure on mesenchymal stem cells (MSCs) chondrogenesis. Another objective was to improve cell adhesion and chondrogenesis on chitosan by modifying the chemical composition of the scaffold (reacetylation, collagen II, or hyaluronic acid (HA) coating). A replica molding technique was …
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Developing osteoarthritis treatments through cartilage tissue engineering and molecular imaging
… can differentiate into chondrocytes, undergo chondrogenesis, and produce cartilage tissue. So far, differences in cell morphology that characterize chondrogenesis had been observed in peptide hydrogels like KLD and RAD but not in the 2.0% agarose hydrogels typically used for culture. A tissue …
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