Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"cholangiocytes"”.
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Epithelial cell plasticity in chronic liver disease (MASLD/NAFLD)
… of interest is the transdifferentiation of cholangiocytes into hepatocytes via either direct differentiation or via a progenitor cell population (Michalopoulos & Khan, 2015). However, the reality of this process in a disease context especially in human remains to be fully demonstrated while …
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A THREE DIMENSIONAL MODEL OF HUMAN HEPATO-BILIARY ORGANOGENESIS
… of differentiating into hepatocytes and cholangiocytes. To effectively use these cells for liver tissue engineering, it is critical to understand their developmental biology, ideally in an in vitro system. The goal of this thesis project was to generate a 3D in vitro model of liver tissue …
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The Immunogenicity of Cholangiocyte Cellular Therapies
Cholangiocytes are the epithelial cells of the biliary system, responsible for the transport and modification of bile. Cholangiocyte disorders, known as cholangiopathies, are a diverse group of life-threatening conditions characterised by cholestasis, ductopenia and eventual liver failure. There …
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Modelling human liver bud development in vitro
… (KRT19+CDX2+PDX1+HNF1B+) and extra-hepatic cholangiocytes (ACE2+MUC13+). By performing extensive snRNAseq analyses across HepBud formation, I was able to validate that hepatic specification in HepBuds follows a natural developmental path forming hepatoblast which strongly resemble 5/6pcw …
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Investigating the role of primary cilia loss in intrahepatic cholangiocarcinoma
… from the epithelial cells lining bile ducts (cholangiocytes), where they serve as sensors of bile composition, flow and osmolality. PC malformation has been reported in cholangiocarcinoma (CCA), a neoplasia that originates from these biliary epithelial cells. Despite changes in cilia …
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TUMOR-PROMOTING FUNCTION AND MOLECULAR REGULATION OF TSPAN8 IN LIVER CANCER
… we found that TSPAN8 is highly expressed in cholangiocytes but absent in hepatocytes in the healthy liver. Remarkably, its expression is robustly switched on in transformed hepatocytes in two liver cancer models induced by DEN and overexpression of Neu respectively. Moreover, germline …
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Mapping Human Liver Development using Single-Cell Transcriptomics to Reveal Pathways Directing Stem Cell Fates
… form an extensive network with non-parenchymal cholangiocytes, endothelial cells, Kupffer cells, and hepatic stellate cells. Disorders affecting hepatocytes are life threatening and increasing in prevalence12, with end-stage treatment relying upon a limited supply of liver donations for …
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Engineering Liver and Cardiac Models for Drug Development and Regenerative Medicine
… cell-cell interactions between hepatocytes and cholangiocytes. This lack of reliable in vitro models incorporating both cell types also leads to unpredicted biliary drug toxicity and inadequate cholangiopathy treatments. Platforms mimicking the bone marrow microenvironment for expansion of …
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Creation of organoid systems to model human endodermal organ development in vitro
… bipotential capacity to generate hepatocytes and cholangiocytes. Furthermore, these organoids were then transplanted into mice and were able to differentiate into complex functional tissues. Next, a newly discovered stem cell was characterised and termed the ‘biliary progenitor cell’. This new …
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Snail1-mediated Gene Repression Links EMT to Enhanced Biliary Epithelial Cell Survival and Lung Epithelial Cancer Cell Migration
… non-malignant murine biliary epithelial cells (cholangiocytes, 603B cells) and human lung epithelial carcinoma cells (A495 cells). We examined collagen production and cell survival in 603B cells and cell migration in A549 cells during TGF-β1-induced EMT. The involvement of Snail1 in mediating …
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Vacuolation, proliferation and neoplasia in the liver of Boston Harbor winter flounder (Pseudopleuronectes americanus)
… that affected preductular cells, hepatocytes, cholangiocytes, neoplastic cells, and exocrine pancreatic cells. To assess the role of vacuolated cells in the progression to neoplasia, evidence for replicative nuclear DNA synthesis was sought by assaying for the nuclear incorporation of a …
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The role of the mechanical microenvironment in regulation of cholangiocyte specification and tubulogenesis
Emerging evidence highlights the influence of the extracellular matrix (ECM) on cell fate and functionality associated with ageing, development and disease (Segel et al. 2019; Bansaccal et al. 2023). Despite most studies emphasising ECM stiffness, recent insights suggest a cooperative role of ECM …
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Engineered microenvironments for studying liver progenitor differentiation
The bipotential differentiation of liver progenitor cells underlies liver development and bile duct formation as well as liver regeneration and disease. Both TGFβ and Notch signaling are known to play important roles in the liver progenitor specification process and subsequent tissue morphogenesis. …