Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"chloroplast transformation"”.
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An investigation into the chloroplast transformation of wheat, and the use of a cyanobacterial CCM gene for improving photosynthesis in a C3 plant
… of wheat yields, and the genetic manipulation of chloroplasts for photosynthetic improvement has many potential advantages over transformation of the nuclear genome. The genetic modification of the chloroplast genome via transformation was first demonstrated in the late 1980’s, and since then, …
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Pentatricopeptide proteins as tools for chloroplast engineering in Marchantia polymorpha
… for genome engineering both at the nuclear and chloroplast level. Along with rapid regeneration that enables the facile generation of large numbers of nuclear-transformants, and rapid progression to homoplasmy during chloroplast transformation. Additionally, its planar morphology is particularly …
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Chloroplast Biotechnology in Higher Plants: Expressing Antimicrobial Genes in the Plastid Genome
… hampered acceptance of GM crops in some markets. Chloroplast transformation presents an attractive alternative to nuclear transformation and offers the potential to ameliorate these environmental concerns. Most agronomically important species exhibit maternal inheritance of organellar genomes …
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Markerfreie transplastome Tabakpflanzen
The genetic engineering of higher plant chloroplasts typically involves the stable integration of antibiotic resistance genes as dominant selectable markers. The potential spread of these genes through pollen release or to soil microbes via horizontal gene transfer is of considerable environmental …
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Expression Of Hepatitis C Viral Non-structural 3 Antigen In Transgenic Chloroplasts
… and replication. Vaccine antigen production via chloroplast transformation system usually results in high expression levels and eliminates the possibility of contamination with vector sequences,human or animal pathogens. The HCV NS3 antigen was expressed in the chloroplast of Nicotiana tabacum …
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Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts
… expressed and orally delivered via plant cells. Chloroplasts have the machinery to fold complex and biologically active eukaryotic proteins in the soluble chloroplast stromal compartment. Protein expression through chloroplast transformation system offers a number of advantages over nuclear …
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Can Plant Plastid Produce a Candidate Influenza Vaccine?
… of ten gene constructs were introduced into the chloroplast genome by biolistic bombardment. Transformants were generated and confirmed through molecular analyses. Transplastomic lines generated with the 14aaRBCL/HA and 14aaGFP/HA constructs accumulated a recombinant protein that was smaller than …
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Nanoelectroporation of single cell plants
… of high value biologics, through the use of chloroplast expression. One of the major bottle necks to scale is low chloroplast transformation rates. This work investigates the adoption of nanotechnology and novel delivery mechanisms to plant transformation, in order to deliver cargo into M. …
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Expression Of Cholera Toxin B Subunit-rotavirus Nsp4 Enterotoxin Fusion Protein In Transgenic Chloroplasts
… mucosal immunization. Protein expression through chloroplast transformation system offers a number of advantages like high level of transgene expression, transgene containment via maternal inheritance, lack of gene silencing and position effect due to site specific gene integration and also the …
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Development and application of novel genetic transformation technologies in maize (Zea mays L.)
… beside its several other advantages, plastid transformation provides transgene containment, and therefore, is an environmentally friendly approach for genetic engineering of crop plants. Reliable in vitro regeneration systems allowing repeated rounds of regeneration are of utmost importance to …
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Analysis of cis-acting expression determinants of the tobacco psbA 5’UTR in vivo
Chloroplast gene expression is predominantly regulated at the posttranscriptional levels of mRNA stability and translation efficiency. The expression of psbA, an important photosynthesis-related chloroplast gene, has been revealed to be regulated via its 5’- untranslated region (UTR). Some …
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Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect
… production of PA83, in plants through chloroplast genetic engineering might eliminate the concerns of adverse side effects and the levels of expression would ensure the availability of vaccine for the human population in an environmentally friendly approach. The primary concern is …
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Chloroplasts as bioreactors : high-yield production of active bacteriolytic protein antibiotics
Plants, more precisely their chloroplasts with their bacterial-like expression machinery inherited from their cyanobacterial ancestors, can potentially offer a cheap expression system for proteinaceous pharmaceuticals. This system would be easily scalable and provides appropriate safety due to …