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Showing 1 to 20 of 23 for “"chitinases"”.
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Development of transition state analogues targeting chitinases and oligosaccharyl transferase
… sequence of a nascent polypeptide. Chitinases, which was emerged as a therapeutic target in combating asthma, are [beta]-1,4-N-acetylglucosaminidases that hydrolyze chitin to generate the disaccharide chitobiose Although the reactions catalyzed by the two enzymes follow different …
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Isolation and Characterization of Chitinases From Dasypus Novemcinctus, the Nine-Banded Armadillo
… was investigated. Both indigenous and endogenous chitinases were studied. Armadillos were collected and samples of their gastric and pancreatic tissues were removed. Tissues were lyophilized and stored at -20° C until they could be resuspended in the appropriate buffers. The extract from …
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Interactions of Chitin and Lignin Thin Films with Other Molecules
… (AFM). Interactions between chitin and family 18 chitinases are vital for understanding bacterial invasion of fungi and human defense against fungal infection. Regenerated chitin (RChitin) thin films were prepared via chemical conversion and spin-coating. Changes in their mass and viscoelasticity …
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Characterization of the chitinolytic system during the mycoparasitic interaction between Trichoderma aggressivum f. aggressivum and different host strains of Agaricus bisporus /
… fungi, is the secretion of lytic enzymes such as chitinases, which actively degrade a host's cell wall. Therefore, the intent of this study was to examine the production of chitinase enzymes during the host-parasite interaction of Agaricus bisporus (commercial mushroom) and Triclzodemza …
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PCR-based cloning, characterization, and stress-induced expression of chitinase genes in Kentucky bluegrass (Poa pratensis L.)
… component of the cell walls of many fungi. Plant chitinases have been implicated in plant defense against pathogens. In plants, chitinase often exists as isoforms and three classes of chitinases have been proposed. In this study, isolation, characterization and expression of chitinase genes in …
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"Sugar Coat" Genetic & chemical validation of <i>Aspergillus fumigatus</i> cell wall targets
… by glycoside hydrolase family 18 plant-type chitinases. In contrast, exploitation of glucosamine-6-phosphate N-acetyltransferase (GNA1) an enzyme in the highly conserved UDP-GlcNAc biosynthetic pathway is an elegant means of targeting chitin synthesis upstream by inhibition of the sugar …
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Regulation of Glucanohydrolases in Nicotiana Tabacum on the Messenger-Rnalevel (beta-1,3-Glucanase, Chitinase, Cytokinin, Auxin, Complementary-Dna Clone)
… and a ca. 34kd precursor for the mature 32-34kd chitinases.
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Biogeochemical and phylogenetic signals of Proterozoic and Phanerozoic microbial metabolisms
… a previously unrecognized HGT involving chitinases (ChiC/D) distributed between fungi and bacteria (∼650 Ma). This HGT is used to tether fossil-calibrated ages from within fungi to bacterial lineages. Molecular clock analyses show that multiple clades of bacteria likely acquired chitinase …
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Biogeochemical and phylogenetic signals of Proterozoic and Phanerozoic microbial metabolisms
… a previously unrecognized HGT involving chitinases (ChiC/D) distributed between fungi and bacteria (~650 Ma). This HGT is used to tether fossil-calibrated ages from within fungi to bacterial lineages. Molecular clock analyses show that multiple clades of bacteria likely acquired chitinase …
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Characterization of Chitinase Activity and Gene Expression in Muskmelon Seeds
… Taken together, these observations indicate that chitinases are regulated developmentally and in a tissue-specific manner in muskmelon seeds. Therefore the potential function of chitinases in muskmelon seeds is discussed. Two complete cDNAs, Cmchi1 and Cmchi2, and a partial genomic clone of Cmchi2 …
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Production and characterization of the recombinant wheat chitinase Wch1 and generation of chitin-specific antibodies
… Wch1 enzyme was found to belong to family 19 chitinases. The enzymatic mode of action resolved by TLC, HPLC and 1HNMR analysis of released oligomers following hydrolysis of (GlcNAc)6 showed that Wch1 inverts the anomeric conformation of newly formed reducing ends and possessed the subsite …
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Secretion and regulation of the chitinolytic machinery in <i>Serratia marcescens</i>
… To achieve this S. marcescens secretes three chitinases (ChiA, ChiB and ChiC) as well as a chitin binding protein (CBP21) that together with the periplasmic chitobiase (Chb) form the chitinolytic machinery. <br/>Research in this group had identified two genes (chiW and chiX) encoding a …
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Solution and solid phase synthesis of N,N'-diacetyl chitotetraoses
… of the substrate specificities and mechanism of chitinases. In this report, we describe the chemical synthesis of three chitotetraoses namely GlcNAc-GlcN-GlcNAc-GlcN, GlcN-GlcNAc-GlcNAc-GlcN and GlcN-GlcN-GlcNAc-GlcNAc. Benzyloxycarbonyl (Z) and p-nitrobenzyloxycarbonyl (PNZ) were used for the …
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Investigating the key components of the chitinase secretion system in <i>Serratia marcescens</i>
… and these are essential for secretion of chitinases. Using X-ray crystallography, the structure of full-length ChiX was resolved to 1.34 Å, revealing structural similarities to the lysostaphin-type (LAS) family of peptidases. Purified ChiX was shown to possess L-Ala D-Glu endopeptidase …
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Screening and evaluation of bacteria for the biological control of Botrytis cinerea on Vitis vinifera
… wall degrading enzymes (glucanases, proteases, chitinases), evaluating competition for space and nutrients via siderophore production on chrome-azurol S agar, and conducting an in vivo assay on wounded grape berries. Antibiosis was evaluated by assessing the effect of the cell-free supernatant …
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Biological wastes as plant growth substrate amendments
… and induced plant defense proteins, namely chitinases and cellulases. Protective effects were seen in all crop models but the results indicate that further trials are required to confirm long-term efficacy. The second part of the research investigated the persistence of enteric bacteria in …
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Comparative genomics of bacteria from amphibian skin associated with inhibition of an amphibian fungal pathogen Batrachochytrium dendrobatidis
… as well as amino acid sequences, of predicted chitinases found in two isolates belonging to the genus Agrobacterium that varied in their inhibition of Bd. After examining the annotated genomes, we identified a predicted chitinase in a Sphingomonas isolate that inhibited the growth of Bd that …
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Secretion of the chitinolytic machinery in Serratia marcescens
… are essential for the secretion of all three chitinases as well as Cbp21. A genetic screen identified genes encoding a holin-like membrane protein (ChiW) and a putative L-alanyl-D-glutamate endopeptidase (ChiX). Subsequent quantitative proteomics experiments and biochemical analyses …
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The role of podovirus-like bacteriophage in the virulence of Vibrio harveyi strain 47666-1
… based on SDS-PAGE, up-regulation of haemolysins, chitinases and greater mortality to larvae of P. monodon. It is therefore suggested that the presence of this bacteriophage may either partly or fully confers virulence to V. harveyi strain 47666-1.
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Soybean mosaic virus-soybean interactions: molecular, biochemical, physiological, and immunological analysis of resistance responses of soybean to soybean mosaic virus
… two subgroups. The SPR3 group was identified as chitinases (CHN) and classed three distinct subgroups. Like HR, the N response induced a series of defense responses with marked temporal increases, in a bimodal pattern, of phenylalanine ammonia-lyase (PAL) and peroxidase (POX) activities, bimodal …
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