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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"chimeric proteins"”.
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Investigation of Adenylate Kinases Isolated From Mesophilic and Thermophilic Members of the Archaeal Genus Methanococcus
Construction of chimeric proteins between M. voltae and M. jannaschii AKs along with site specific mutational analysis confirm the importance of specific stabilizing interactions identified by sequence comparisons, and allow the physical characteristic of these proteins to be significantly altered. …
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Generation And Statistical Modeling Of Active Protein Chimeras: A Sequence Based Approach
… a valuable tool to probe the functional space of proteins. Statistical modeling is the next logical step, allowing us to build a model of gene fragment replaceability between species. In this thesis I begin to develop the statistical tools that are needed to systematically describe combinatorial …
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CHARACTERIZATION OF HEPATITIS B VIRUS (HBV) IN HEPATOCELLULAR CARCINOMA (HCC) PATIENTS
… 3¿-end of the HBx is often deleted. HBx-human chimeric transcripts, the most common type of chimeric transcripts, can be expressed as chimeric proteins. Sequence variation resulting in non-conservative amino acid substitutions are commonly observed in HBV genome. This study highlights HBV as …
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Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells
… transfer (FRET). These reporters are four-part chimeric proteins with the domains from N- to C-terminus as follows: a cyan fluorescent protein (CFP), a phosphoserine recognition domain (14-3-3 tau) or a methyllysine recognition domain (HP1 or Pc chromodomain), a peptide whose sequence …
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Mechanistic insight on a chimeric Cas9 protein's specificity for DNA target with 5 '-NAA-3' PAM
… Cas nuclease engineering has largely yielded proteins with enhanced specificity for NGG and variations on G-containing PAMs, we were able to construct a chimeric Cas protein with consistent specificity for a 5'-NAA-3' PAM by rationally combining the PAM-interacting domain of Streptococcus …
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Mechanism of yeast prion portein aggregation and strain formation
… formation. We previously determined that these chimeric proteins maintain the [PSI+] yeast prion phenotype. Interestingly, we noted that the repeat expanded chimeric prions maintained a stronger strain of [PSI+]. Investigations of the chimeric proteins in vitro revealed that repeat-expansions …
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Studies on the structural organization of Golgi complex
… modification and sorting of secretory proteins. We have characterized the distribution in Vero cells of three Golgi cisternal membrane proteins, Sialyltransferase (SialylT, tans- Golgi/TGN), galactosyltransferase(GalT, trans-Golgi/TGN), and Nacetylglucosametransferase- 1 (GlcNAcT-1, …
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Diagnostic and prognostic aspects of anti-neutrophil cytoplasmic antibodies in systemic vasculitis
… nodosa, were described. Recombinant chimeric molecules were produced in order to map epitopes of monoclonal antibodies to PR3. The clinical utility of these chimeric proteins in the diagnosis and follow-up of patients with PR3-ANCA positive vasculitis was studied and these chimeric …
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The introduction of incompatibility between Junin virus Z protein and other viral proteins results in impaired replication and attenuation
… LP is comprised of four domains. We generated chimeric LPs representing permutations of Can and Rom to determine which Rom domain exhibited an incompatibility with Can Z. The in vitro characterization results of these chimeric proteins indicated that both domains I and III likely contribute to …
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EWSR1 and FUS fusion genes in tumorigenesis
… of EWSR1 and FUS fusion genes, and the chimeric proteins they encode, in tumorigenesis. In Article I, the EWSR1 promoter, which regulates the expression of EWSR1 fusion genes, was characterized and regions that were crucial for promoter activity could be identified. The sequence was …
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Engineering LuxR-type quorum sensing proteins for new functions
… module are not modular. Here, we develop chimeric proteins that can accept the small molecule cognate belonging to the las operon from Pseudomonas aeruginosa while activating the cognate promoter of other quorum sensing systems. The ability to swap in a modular fashion the ligand-binding …
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Principles for the design and construction of synthetic circuits utilizing protein-protein interactions in Saccharomyces cerevisiae
… this scheme, design principles of modular chimeric proteins, engineered pathway convergence, and phosphorylated activated localization are developed and subsequently applied to create phosphoin/phospho-out composable protein devices in yeast. The devices implement BUFFER, NOT and OR logical …
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Significance of Protein Interactions in Mediating AF9 Function
… prognosis. MLL gene rearrangements give rise to chimeric proteins that contain the N-terminal portion of MLL fused to the C-terminal portion of over 50 different fusion partners. The chimeric proteins cause constitutive expression of some MLL target genes such as HOXA9 and MEIS1, and enhanced …
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A signal for retention of cytochrome P450 2C1 in the endoplasmic reticulum
… coli alkaline phosphatase, and the hybrid proteins were expressed in COS1 cells and cellular localization was determined by subcellular fractionation, immunolocalization and the glycosylation state of the proteins. Both the $\beta$-galactosidase and alkaline phosphatase hybrid proteins were …
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Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… VEGF-A). Here, firstly we engineered fusion proteins that generate a Ca2+ signal upon ligand binding by creating fusions of domains that oligomerize to the transmembrane domain and the cytoplasmic tail of the VEGFR2. By coupling these chimeric proteins that generate Ca2+ signals with proteins …
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Engaging Immune Cells within the Tumor Microenvironment to Enhance Efficacy of Oncolytic Virotherapy
… engaging the immune cells within the TME, using chimeric engagers for improved anti-tumor efficacy. To accomplish this, we have generated two chimeric proteins, the Bispecific and Trispecific engagers (BiCEP and TriCEP), the genes encoding for which were delivered by an HSV amplicon system, …
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The Role of Phosducin-like Protein and the Cytosolic Chaperonin CCT in G beta gamma dimer Assembly
… CCT were determined by testing various PhLP/Pdc chimeric proteins in the CCT binding assay. In the other part of this dissertation, the physiological role of PhLP in G protein signaling was investigated. Cellular expression of PhLP was blocked using RNA interference targeting PhLP. Together with …
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Reengineering Estrogen Receptor Hormone Specificity Through Coordinated Changes at Key Contact Sites Between Estrogen Receptor and Estradiol
… for the regulation of transgenes, we have made chimeric receptors that combine the DNA binding domain of Gal4 with the ligand binding domain of wild type, E353S or E353A mutant ER and the activation domain of VP16. These chimeric proteins were tested in mammalian cell transfection assays, and …
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Biochemical characterization of calmodulin isoforms and identification of calmodulin-binding proteins in Arabidopsis
… max}$, were observed between CaM isoforms and chimeric proteins tagged at their N-termini with a 12-residue c-myc epitope. Nonsense mutations of the C-terminal 7 residues of CaM-6 (CaM-6M), however, drastically impaired the ability of the protein to activate NAD kinase. The requirement for free …
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A COMPARATIVE ANALYSIS OF INTRACELLULAR CHOLESTEROL ESTERIFYING ENZYMES IN MAMMALS
… study, putative active site residues of ACAT proteins were identified using chemical modification and site directed mutagenesis approaches. A conserved region within the protein was defined as the putative active site domain based on highly conserved sequence similarities from yeast to humans. …
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