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Showing 1 to 20 of 48 for “"chemostat"”.
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Mathematical analysis of fractional-order chemostat model with time delay
… studied to describe the dynamic behaviour of a chemostat system. This study also investigated the FDEs with time delay to examine the effect of time delay on the behaviour of a chemostat system. The integer-order chemostat model in the form of the ordinary differential equation (ODEs) is …
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Dynamic behavior of phytoplankton populations far from steady state : chemostat experiments and mathematical modeling
Nature changes continuously and is only seemingly at equilibrium. Environmental parameters like temperature, humidity or insolation may strongly fluctuate on scales ranging from seconds to millions of years. Being part of an ecosystem, species have to cope with these environmental changes. For …
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Mathematical Modeling and Disease Related Applications: A New Method of Estimating Bacterial Mutation Rates, Dynamics of Killer Yeast in a Chemostat, and Other Problems
… culture under nutrient limitation (e.g. a chemostat). This research explores possible detrimental effect on fitness when yeast harbor the killer virus under chemostat conditions and when a non-killer yeast strain can out compete a killer-strain of yeast.</p>
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Mathematical Modeling and Disease Related Applications: A New Method of Estimating Bacterial Mutation Rates, Dynamics of Killer Yeast in a Chemostat, and Other Problems
… culture under nutrient limitation (e.g. a chemostat). This research explores possible detrimental effect on fitness when yeast harbor the killer virus under chemostat conditions and when a non-killer yeast strain can out compete a killer-strain of yeast.</p>
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Studies on survival of Pseudomonas aeruginosa 6750
… on the survival of P.aeruginosa in batch and chemostat studies. Survival or percentage culturability, as measured by total and colony count ratio, was found to decrease both in extended batch culture and for chemostat cells with decreasing growth rate. Extended batch culture, however, did not …
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Elucidating Factors that Impact the Removal of Organic Microconstituents by Ammonia Oxidizing and Heterotrophic Bacteria
… was biotransformed but not mineralized by AOB chemostat and batch cultures. TMP was not transformed by AOB batch or chemostat cultures; however both EE2 and TMP were transformed by Ox⁻ and Ox⁺ chemostat cultures. Radiolabeled studies showed that EE2 was mineralized by this culture. Kinetically, …
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Terminal anerobic interactions in a microbial association isolated from landfill
A multi-stage (5-vessel) chemostat was used to enrich and isolate from landfill a microbial association capable of dissimilating hexanoic acid (5 mM) under anoxic conditions. Two possible catabolic mechanisms existed : (i) that hexanoate was mineralised completely to carbon dioxide, although this …
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The physiology of industrial yeast in continuous culture
… yeast) was studied under glucose-limitation in chemostat culture. Levels of lg/1 (0.1% w /v) glucose allowed cell growth while preventing fermentation in a defined medium (QEMM3). Metabolism of glucose by respiration or fermentation was shown to affect the mean cell volume, with fermentative use …
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Modeling dual-limitation kinetics incorporating intracellular cofactor responses
… putida P$\sb{\rm p}$F1 performed in a special chemostat apparatus, using acetate as electron donor and O$\sb2$ as electron acceptor, revealed that the cofactor concentrations vary highly systematically with changes in availability of the external electron donor and acceptor. In general, the …
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Investigation of Factors Influencing Niche Differentiation of Ammonia-oxidizing Archaea and Bacteria in Freshwater Environments
… factors driving niche separation of AOA and AOB. Chemostat competition experiments were conducted using one representative AOA and AOB under ammonia-limiting, high oxygen (21%) and ammonia-limiting, low oxygen (1%) conditions. In all cases, the AOA outcompeted the AOB for ammonia, and the AOB was …
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The influence of magnesium ions on the growth and metabolism of <i>Saccharomyces cerevisiae</i>
… range of growth rates using a magnesium-limited chemostat was carried out with the results agreeing in the main with the trends established in the batch cultures. At growth rates lower than μ<sub>max</sub>, the cells reveal a physiological state characteristic of non-carbon-limited growth. This …
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Plasmid stability of pUB110 and pUB110-derived plasmids in Bacillus sphaericus 2362
… after 40 generations of vegetative growth in a chemostat. Each plasmid-bearing strain was grown at both 28°C and 38°C, with generations times of 42 min and 108 min. All plasmids were functionally stable under the conditions of extended vegetative growth that were successfully tested. The strain …
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Factors that enhance the ability of Pseudomonas aeruginosa to resist the action of antibiotics
… was most readily developed. From these results, chemostat studies were designed to examine prolonged exposure of the antibiotic to planktonic cells. Chemostat cultures were exposed to amikacin at sub-inhibitory concentrations using Evans defined synthetic medium at different dilution rates (D) …
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Physiological response of bacillus licheniformis NCIMB 8874to oligosaccharide elicitors
… enzyme ß-mannanase. MO (300 mg L-1) supplemented chemostat cultures of B. licheniformis did not result in a significant change in bacitracin A concentration and ß-mannanase activity compared to the control fermentation. However, when the chemostat culture was changed to batch, increases were …
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The effects of nutrient limitation and growth rate on the regulation of the multi-drug pump genes acrAB in escherichia coli
… in these studies and it has been established by chemostat culture that the expression of acrAB was inversely related to growth rate, and this was irrespective of the limiting nutrient. This regulation was not OS_dependent as the expression of acrAB increases during stationary phase in the batch …
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Microscale controlled continuous cell culture
… on a single device, such as batch, fed-batch, chemostat, and turbidostat continuous culture. Validation experiments demonstrate that cells can be grown to high optical densities (OD = 50) and production of commercially relevant chemicals such as DNA vaccines are comparable to large scale bench …
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Partitioning Between Soil-Adsorbed and Planktonic Escherichia coli
… to inoculate these samples was cultured using a chemostat reactor to control cell growth stage and control variability. A linear isotherm (R²=0.88) was selected to describe this experimental data; however, future studies characterizing the partitioning behavior of <I>E. coli</I> under different …
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Some nonlinear problems in plankton ecology
… resource control of cell cycle progression, into chemostat models for the phytoplankton-nutrient interaction. Simulation results demonstrate that the· population structure can generate oscillatory dynamics under a constant nutrient supply, and that such oscillations are important to population …
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Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
… to even higher PHB fluxes.In a nitrogen-limited chemostat, PHB flux did not change with dilution rate. Unlike prior pleiotropic perturbations, these systematic experiments could clearly conclude that the flux control is in the PHB pathway. At high PHB flux, growth rate was extremely hindered and …
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Tools and reference standards supporting the engineering and evolution of synthetic biological systems
… published microscope-mounted microfluidic chemostat.
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