Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"chemogenetics"”.
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Chemogenetics, Induced Neurons and Pluripotent Stem Cells: Towards Advanced Gene and Cell Therapies Targeting Epilepsy
The complexity of the central nervous system and existence of the blood-brain barrier often causes difficulties for traditional pharmacological treatments of neurological diseases. This thesis explores the feasibility and potential for novel gene and cell therapy approaches, which hold better …
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Hypothalamic control of energy rheostasis during metabolic challenges, pregnancy, and lactation
… using cre-lox system mediated gene deletion, chemogenetics, and in situ hybridization in adult mice. MC3R signaling in the DMH was found to contribute to energy rheostasis, with region-specific deletion altering food intake and body weight in response to high-fat diet feeding or …
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Hippocampal Output to the Septum Regulates Locomotion and Exploration
… and unclear. By using optogenetics and chemogenetics, we selectively manipulated the DH pan-inhibitory interneurons (INs) and revealed that disrupting the DH inhibitory circuit is sufficient to acutely induce hyperlocomotion. Furthermore, we found that the DH-septum inhibitory pathway is …
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Investigation of Accessory Olfactory Interneuron Physiology and Function After Experience-Dependent Plasticity
… Using a combination of ArcTRAP mice and chemogenetics (Cre-dependent hM4D(Gi)-mCherry AAV injections), I found that disrupting Arc-expressing IGC activity during repeated resident-intruder interactions abolished the ramping aggression exhibited by resident male mice. This work shows that …
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The Role of Glutamate in the Medial Prefrontal Cortex in the Acquistion and Expression of Conditioned Approach
… glutamatergic neurons. The final experiment used chemogenetics to inhibit glutamatergic projections from dmPFC to NAc, finding a significant impairment in conditioned approach expression. Confocal microscopy revealed the pathway originating from dmPFC glutamatergic cells to NAc, supporting the …
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Investigating the neurobiology regulating cognitive effort allocation using a rodent model of cost/benefit decision making
… less attentional demand. In experiment 1, I use chemogenetics to downregulate cholinergic neurons of the basal forebrain as rats perform the rCET, to determine whether this neuronal population is responsible for the previously ascribed role of acetylcholine in regulating decision making with …
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Hypothalamic Nutrient Sensing
… roles in controlling glucose homeostasis. Using chemogenetics to selectively activate these neurons, I demonstrated, in contrast to a similar, recently published study, that blood glucose concentrations did not rise upon activation of these neurons. A subpopulation of AgRP neurons express …
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Spatiotemporal Dynamics of Tissue Oxygenation in Awake, Behaving Mice
… vascular tone directly using mural cell Gq-chemogenetics, this dramatically limited both the magnitude and spatial properties of the evoked hemodynamic response and instead intensified vasomotion. My data suggest that the late component of sustained functional hyperemia in awake mice …
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Multifunctional Wireless Gut-Brain Neurotechnology
… edge neurobiological tools like optogenetics, chemogenetics, and activity-based sensors have revolutionized studies of the brain. However, application of these methodologies for studies of brain-body circuits is reliant on engineered devices that support these sophisticated functions in …
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A multipronged approach to understanding how genes contribute to social behavior in threespine stickleback
… system, and is the first step toward using chemogenetics and optogenetics.
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Neural and psychological mediators of trait impulsivity: cortico-striatal circuitry and catecholaminergic neuromodulation
… (intra-cerebral) pharmacology, brain lesions and chemogenetics. In chapter 2, I show that premature responses are influenced by reinforcement rate and motivation, and that negative urgency does not seem to play an important role in the genesis of these responses. In chapter 3, I found that high …
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Exploring the role of Gastrin-Releasing Peptide Neurons in Circadian Time-Keeping of the Suprachiasmatic Nucleus
Circadian (approximately one day) rhythms help organisms predict, and thereby adapt to, solar time, ensuring efficient use of energy and resources. In mammals, circadian rhythms are present in practically every cell of the body and are synchronised by a master clock, the hypothalamic …