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Showing 1 to 18 of 18 for “"chemiluminescence detection"”.
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CLINICAL APPLICATIONS OF CHEMILUMINESCENCE DETECTION
<p>This dissertation evaluates the potential of chemiluminescence analysis for two types of clinical applications: (1) rapid analysis with a flow injection system which consumes small reagent volumes (25 (mu)L) and (2) a new approach to chemiluminescence immunoassay.</p><p>The first application …
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Miniaturised analytical systems with chemiluminescence detection for environmental applications
… details the use of microfluidic devices and chemiluminescence detection in order to develop a portable method of analysis for measuring chemical species in the environment.Chapter 1 outlines microfluidic technology, including fabrication techniques, fluid manipulation and mixing within the …
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DETERMINATION OF TRACE METALS BY lON-CHROMATOGRAPHY WITH CHEMILUMINESCENCE DETECTION
It is generally considered that the detection step for the determination of trace metals by liquid-chromatographic systems is the weakest area of the analytical procedure. Various post-column reagent systems, for trace metal analysis, based on the highly sensitive luminol-peroxide chemiluminescence …
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Investigations of Chemiluminescence Detection Parameters for Kinetic Analysis and Capillary Separations
A post-column CL detection method for capillary electrophoresis (CE) was developed in which the capillary effluent was collected on a membrane which had been coated with bis(2,4,6-trichlorophenyl)oxalate, a CL reagent. After the CE run, the CL reaction was initiated on the membrane by applying …
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FLOW SYSTEMS FOR CLINICAL ANALYSIS USING IMMOBILIZED ENZYMES AND CHEMILUMINESCENCE DETECTION (CHROMIUM, GLUCOSE)
… in this study are two flow systems based on chemiluminescence (CL) detection that provide the features important in a practical clinical method: a flow injection analysis (FIA) system and a two phase flow system. Both systems take advantage of the specificity of enzyme reactions.</p><p>FIA …
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DISSOLUTION OF AEROSOL IRON IN SEA WATER USING FLOW-INJECTION WITH CHEMILUMINESCENCE DETECTION
… in seawater were investigated using FI-CL detection. A manual FI manifold was automated and optimised for the determination of iron, at nanomolar concentrations, released from aerosols in seawater. The limit of detection was 0.04 ± 0.03 nM and the precision (rsd) ranged from 0.2 to 10.8%. …
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THE DETERMINATION OF COPPER IN SEA WATER USING FLOW INJECTION WITH CHEMILUMINESCENCE DETECTION
… and shipboard deployment of a flow injection - chemiluminescence (FI-CL) technique for the determination of labile Cu(II) and total copper (by UV irradiation) in seawater. The operational parameters of the FI manifold in a UHP water sample matrix and the 1,10-phenanthroline CL reaction were …
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Flow Injection Determination of Sugars by Combining Immobilized Enzyme Reactors and Chemiluminescence Detection
Chemiluminescence has long been known as a sensitive means of detection, but analytical applications have been limited due to a lack of selectivity. There has been much interest in recent years in combining chemiluminescence detection with the inherent selectivity of enzyme reactions. The …
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DETERMINATION OF CO AND MN IN MARINE WATERS USING FLOW INJECTION WITH CHEMILUMINESCENCE DETECTION
… and shipboard deployment of a flow injection - chemiluminescence (FI-CL) technique for the determination of cobalt (Co) and manganese (Mn) in seawater. Chapter One presents an overview of the marine environment and the biogeochemistry of Co and Mn. Current analytical methods for the …
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Chemiluminescence detection with a regenerable reagent by using tris(2,2'-bipyridyl)ruthenium(II) immobilized in nafion
… a regenerable sensor based on electrogenerated chemiluminescence from tris(2,2$\sp\prime$-bipyridyl)ruthenium(II) (or Ru(bpy)$\sb3\sp{2+})$ immobilized within a polymer (Nafion) coated on an electrode. Via control of the electrode potential creation of the reactive reagent Ru(bpy)$\sb3\sp{3+}$ …
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Chemiluminescence detection of NADH and enzyme substrates with immobilized tris(2,2'-bipyridyl)ruthenium (II) and dehydrogenase enzymes
… (Ru(bpy)$\sb3\sp{2+}$) electrogenerated chemiluminescence (ECL) to the selectivity of dehydrogenase enzymes. Within this objective, methods of reagent immobilization for Ru(bpy)$\sb3\sp{2+}$ and dehydrogenase enzyme have been studied and applied to bioanalytical determinations. …
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The evaluation and the application of array detectors for analytical luminescence spectroscopy.
… charge-coupled device (CCD), for low-light level chemiluminescence and other luminescence spectroscopies. This research conclusively demonstrates the superior qualitative and quantitative performance of spectrometric systems which employ these detectors. It is experimentally shown that a single …
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Oxidative, inflammatory and vascular factors in Alzheimer's disease
… tissue, 1D and 2D PAGE, western blotting with chemiluminescence detection, ELISA assays of Abeta 1-40 and 1-42, quantitative ECNI GCMS of o- and m-tyrosine as well as metabolites of the kynurenine pathway, quantitative MALDI-TOF assay of hemorphins and LCMSMS based proteomics, to identify …
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Speciation of chromium in environmental matrices.
… acid followed by on-line ion chromatography with chemiluminescence detection was found to be simple, sensitive, free of interferences, and more importantly the integrity of the chromium species was maintained. Instrumental parameters affecting the sensitivity of the chemiluminscence signals were …
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Development of novel methods for the detection of Vibrio parahaemolyticus in seafood
… Currently very few methods are in use for rapid detection of this organism. In addition, these methods are not always specific and can give false positive reactions.This study describes the development of a novel method, based on phage antibody display, for the detection of V. parahaemolyticus in …
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Total Plasma Homocysteine, Vitamins, Abdominal Aortic Aneurysm and Peripheral Vascular Disease
… B<sub>12</sub> and folic acid was analysed using chemiluminescence detection technique. Serum was separated within one hour of blood collection from antecubital venous puncture into an EDTA primed tube, from 38 patients with AAA, 36 patients with PVD and 36 control subjects for analysis of …
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Implementation of a method to determine sub-nanomolar concentrations of iron in seawater and its application to the study of marine iron biogeochemistry at the ocean-shelf interface
… The setting up of a flow-injection analyser with chemiluminescence detection (FIACL) for Fe(II) proved to be non-trivial. Extensive work was undertaken to solve problems relating to our limited level of understanding of the CL reaction, and the variable behaviour of the resins prepared to …
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Supercritical Fluid Chromatography with Chemiluminescent Nitrogen and Sulfur Detection
… no chromophore that can be used for spectrophoto detection. The objective of this research is therefore to interface selective chemilumninescent detectors with SFC in the sensitive detection of nitrogen- and/or sulfur containing compounds. The chemiluminecent nitrogen detector (CLND), a gas-phase …