Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"chemical shift perturbation"”.
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Characterization of an Aptamer Targeting the p47/p97 Complex for Therapeutic and Diagnostic Applications in Neurodegenerative Disorders and Cancer
… localized the interaction to the SEP domain. Chemical shift perturbation and fluorescence assays mapped the protein binding interface and key nucleobases on the aptamer. Importantly, the aptamer retained binding to p47 in the presence of p97, demonstrating compatibility with the native …
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NMR investigations of blood coagulation: Conformational changes of membrane bilayers and proteins in blood coagulation
… of these biological molecules lead to perturbations in their structure and dynamics. Understanding the variations in the constitution of these molecules as the result of functional interactions will provide insights into various biological processes. In this work, we employ solution and …
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Specialization of a calmodulin-like protein: Androcam adopts a single conformation over the entire physiological range of calcium
… Consistent with these structural observations, chemical shift perturbation experiments show that androcam interacts with the unique 'Insert2' peptide of the biological target Myosin VI with its C lobe only, whereas calmodulin binds this target using both lobes. Our results indicate that the …
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Decoding the Sequence-Dependent Properties of Intrinsically Disordered Proteins
… our IDP-dynamic prediction method (SeqDYN) with chemical shift perturbation data to develop DIRseq, a prediction method for identifying IDP drug binding regions. IDPs are known to readily undergo homogenous liquid-liquid phase separation (LLPS) under certain conditions, however variations in salt …
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Structural Modes in Proteins: A Case Study in the PDZ Domain
… between all pairs of residues. This global perturbation analysis is performed by making evolutionarily conservative mutations at every position in a protein and observing physical effects by monitoring a large number of NMR chemical shifts at nuclei distributed throughout the protein. The …
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Dissecting interactions within focal adhesions: studies on vinculin
… transferred to a wild-type Vt spectrum.<br/>Chemical shift perturbation studies have subsequently been undertaken using wild-type Vt<br/>and paxillin.<br/><br/>The data presented here is consistent with specificity for LD motifs and points to an<br/>interaction between Vt and LD1 and LD2. In …
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Rational design and optimization of anti-Cdc42 peptide inhibitors
… a previously solved structure of Cdc42 and chemical shift perturbation data. The model of the complex indicated peptide binding interactions with the effector binding region of Cdc42. Binding assays demonstrated the ability of the peptides to disrupt downstream Cdc42-effector interactions, …
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NMR studies of RNA binding domains of human lysyl aminoacyl tRNA synthetase
… surface that is affected by RNA binding via NMR chemical shift perturbation techniques. The RNA molecules studied this way include the anticodon stem-loop of lysyl tRNA isoacceptor 3 (ACSL) and a tRNA-like element (TLE) derived from a region 5’ to the primer binding site of the HIV-1 RNA genome …
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Autoinhibition and Chloride Sensing in the WNK1 Kinase
… from the WNK1 kinase domain displays extensive chemical shift perturbation as assessed by 1H,15N-HSQC. Expression of this autoinhibitory domain in cis with the WNK1 kinase domain followed by size-exclusion chromatography shows substantial confor- mational changes when dialized from high to low …
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Specific Binding Affinity of the Non-Catalytic Domain of Eukaryotic Like Type IB Topoisomerase of vaccinia Virus
… rate of relaxation and rapid dissociation. Biochemical data suggests that the N-terminal domain (NTD) is important for pre-cleavage binding and affinity for the target site. A combination of NMR-based interaction studies, the measurement of backbone dynamics using <sup>15</sup>N relaxation …