Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"chemical reporters"”.
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Chemical Reporters for Bacterial Pathogenesis and Beyond
… Towards this goal, I have developed chemical reporters of nucleic acid and protein synthesis and posttranslational modifications to explore their functions in bacterial pathogenesis as well as basic biological pathways. Initially, I developed amino acid chemical reporters that allow …
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Chemical Reporters for Investigating Lipidated Proteins at the Host-Pathogen Interface
… machinery to enhance infection. Robust biochemical methods for characterizing lipidated proteins are therefore important for understanding fundamental physiology and disease mechanisms. In this thesis, I report the development of alkyne-lipid chemical reporters that afford more sensitive …
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Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… analysis of fatty-acylated proteins using chemical reporters undertaken here revealed a greater diversity of lipid-modified proteins in mammalian cells than had previously been appreciated. Proteins targeted by a series of fatty acid chemical reporters ranging from myristic to stearic acid …
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Chemical Tools for Exploring Metabolite Interactions with Nuclear Receptors and Beyond
… interactions remains a major challenge in chemical biology and drug development. The functionalization of native metabolites and synthetic small molecules with bio-orthogonal detection tags (alkyne, azide, and others) has afforded chemical reporters for the biochemical analysis of …
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The Development of Novel Tools for the Metabolic Labelling of Glycans in Cancer
… unnatural sugars bearing bioorthogonal chemical reporters. Methylcyclopropenes are one such reporter with good incorporation and kinetics of the subsequentinverse-electron-demand Diels-Alder reaction (IED-DA) with tetrazines. The methylcyclopropene tagged monosaccharide most often used …
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Develoment and Application of Chemical Strategies to Study Protein Fatty-Acylation in Eukaryotes
… and palmitoylomes, I developed bioorthogonal chemical strategies for improved analysis of dynamic palmitoylation in mammalian cells. I showed that alkyne-functionalized fatty acids, in conjunction with azido-fluorophores, provide the most sensitive detection of acylated proteins following …
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Targeted Imaging Agents for Detecting Tumour Cell Death following Therapy
… module (within 58±5.8 min (n = 12), >98% radiochemical purity and 12±3% decay corrected yield) has been developed. This was used to label the single cysteine present on C2Am within 30 min in PBS (Am=212000±30000 MBq/µmol (n = 3)). Using xenograft models of breast and colorectal cancer, and a …