Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"chemical proteomics"”.
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MS-Based Chemical Proteomics Studies of Extracellular Glycoproteins: Identification, Quantification, and Dynamics
… through mass spectrometry (MS)-based proteomics. First, an enrichment method based on metabolic labeling with a sugar analog, copper-free click chemistry, and MS-based proteomics is employed to target cell-surface glycoproteins from multiple cell lines. The method is universal for all …
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Insights into the mechanism of action of quinoline antimalarials against Plasmodium falciparum revealed by novel fluorescent analogues and chemical proteomics
… was designed and synthesised. Key physicochemical properties of these antimalarials were retained in order to preserve the interactions of these drugs with their putative target, ferriprotoporphyrin IX or Fe(III)PPIX. Versatile derivatisation of the alkaloids was enabled by a …
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Chemical Tools for Exploring Metabolite Interactions with Nuclear Receptors and Beyond
… interactions remains a major challenge in chemical biology and drug development. The functionalization of native metabolites and synthetic small molecules with bio-orthogonal detection tags (alkyne, azide, and others) has afforded chemical reporters for the biochemical analysis of …
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Dopamine Transporter (DAT), Nicotinic Acetylcholine Receptor (nAChR), and Metabotropic Glutamate Receptor 2 (mGlu2) Irreversible Probes For Identifying Anti-Psychostimulant Therapeutics
… of this research was to develop irreversible chemical probes based on promising anti-addiction lead compounds (i.e., pyrovalerone, bupropion, BINA, etc) to map their binding sites and poses within the DAT, select nAChR subtypes, or mGlu2. The central hypothesis was that these compounds could …
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Proteomic Profiling of Pro and Active Matrix Metalloproteinases using Tandem Mass Spectrometry. Optimization of Affinity Chromatography and nHPLC-MALDI-MS/MS for Proteomic discrimination of Matrix Metalloproteinases in pre-clinical Cancer Model.
… The objective of this project is to develop a chemical proteomics strategy based on Matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS) to help identify and discriminate the various MMP forms. Firstly, a triazine dye-based ligand immobilized on chromatography …
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Biochemical and Structural Insights into VCP Unfoldase and WRN Helicase Complexes
… Decades of research have focused on the biochemical and structural principles of VCP function, especially pertaining to its role in the proteostasis network by unfolding polyubiquitylated substrates prior to their recycling or proteasomal degradation. For example, several groups have used …
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Monoamine Transporter Photoaffinity Ligands Based On Methylphenidate and Citalopram: Rational Design, Chemical Synthesis, and Biochemical Application
… dissertation was to develop irreversible chemical probes based on methylphenidate (MP) and citalopram (CIT), two therapeutically significant MAT inhibitors, in order to map their binding sites and poses within their major MAT target protein. The central hypothesis was that MP and CIT could …
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Target Identification and Mechanism of Action Studies in Folate Metabolism in Kinetoplastids
… were immobilized onto resins, followed by chemical proteomics, utilizing novel techniques (iTRAQ), to allow for target identification. Using competition studies, specific and non-specific targets were identified in parasitic lysate (T. brucei and L. major) for each anti-folate compound. …
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Control of Salmonella Virulence by Microbiota-Derived and Dietary Fatty Acids
… remain poorly characterized. We use a chemical reporter strategy to identify molecular targets of short-chain fatty acids in Salmonella. I demonstrate that alkynyl-functionalized short-chain fatty acids can be metabolized and covalently attached to proteins in Salmonella. Proteomic …
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Global characterization of oncogenic kinase signaling
Aberrant activation and expression of the proto-oncogene Src have been observed in various human malignancies. Despite major advancements in kinase-targeted cancer therapies, targeting Src has only achieved limited clinical success, thus highlighting the urgent need to better understand the complex …
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Mechanism of Action Studies of Phenotypic Whole-cell Active Antimalarial Leads Through Target Identification
… parent compounds. The photophysical and physicochemical properties of all fluorescent compounds were evaluated. Although the fluorescent derivatives lost antiplasmodium potency relative to their parent compounds, all but NBD-labelled KP124 retained antiplasmodium activity in the …