Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"chaperonin"”.
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Role of Chaperonin CCT in G-protein Biosynthesis
Chaperonins are ubiquitous molecular chaperones that are found in all animal kingdoms. They all share a common structure and function to assist the folding of other proteins. All chaperonins consist of two stacked rings, which come together to form a central cavity where folding can take place. The …
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Assembly and substrate recognition properties of human CCT subunits of the TRiC chaperonin
Group II chaperonins are large multi-subunit complexes that fold cytosolic proteins to their native structures. They are composed of two back-to-back rings of 7-9 subunits. The eukaryotic cytosolic type II chaperonin Tailless Complex Polypeptide-1 (TCP-1) Ring Complex (TRiC) consists of eight …
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The Role of Phosducin-like Protein and the Cytosolic Chaperonin CCT in G beta gamma dimer Assembly
… suggested to regulate the activity of cytosolic chaperonin CCT by their high affinity interaction. In the present study, the three-dimensional structure of PhLP:CCT complex has been solved by cryoelectron microscopy. PhLP was found to bind only one of the chaperonin rings with both N- and …
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The Role of Phosducin-like Protein as a Co-chaperone with the Cytosolic Chaperonin Complex in Assembly of the G Protein βγ Subunit Dimer
… has been shown to interact with the cytosolic chaperonin containing TCP-1 (CCT), and the βγ subunit dimer of heterotrimeric G proteins (Gβγ). Here we provide details obtained from cryo-electron microscopic and biochemical studies on the structure of the complex between the cytosolic chaperonin …
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The Mechanism of Assembly of the G-Protein Beta Gamma Subunit Dimer by CK2 Phosphorylated Phosducin-Like Protein and the Chaperonin Containing TCP-1
… PhLP has also been shown to interact with the chaperonin containing TCP-1 (CCT) atop its apical domain, not entering the substrate folding cavity. However, the physiological role of the PhLP-CCT interaction in G-protein beta gamma dimer formation remains unclear. This study addresses the …
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Protein-mediated nanocrystal assembly for floating gate flash memory fabrication
… NCs was organized using a self-assembled chaperonin array. The chaperonin array was applied as a scaffold to mediate NCs into an assembly with uniform spatial distribution on Si wafers. By using this method, we demonstrated that colloidal PbSe and Co NCs in suspension can self-assemble …
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Untersuchung der Reaktionszyklen von Chaperoninen aus Escherichia coli und Thermoplasma acidophilum mit Hilfe der Neutronenkleinwinkelstreuung
Untersuchung der Reaktionszyklen von Chaperoninen aus Escherichia coli und Thermoplasma acidophilum mit Hilfe der Neutronenkleinwinkelstreuung Am Thermosom wurden bisher noch keine Komplexe untersucht. Hier war von großem Vorteil, daß bei SANS kein Strahlenschaden auftritt und damit Proben …
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Functional and structural characterisation of Mycobacterial Chaperonins
… are 2 distinct groEL homologues which encode the chaperonins Cpn60.1 and Cpn60.2, with the latter predicted to be the main house-keeping chaperonin. Phylogenetic analysis has revealed that the genes for the duplicated chaperonins diverged a long time ago. This implies that the duplicated …
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Models of neurodegeneration using computational approaches
… expect by chance. Three of these genes form a chaperonin complex and the fourth gene codes for actin, a major substrate of the same chaperonin. Next I have evaluated the circadian disruptions in AD by developing a new system to simultaneously monitor the oscillations of the peripheral molecular …
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Quality control of protein complex assembly
… examples include the ribosome, proteasome, chaperonin, tubulin, haemoglobin, and many others. A major challenge for the cell is to synthesise individual subunits in the correct stoichiometry. Any imbalance in subunit synthesis results in excess unassembled “orphan” subunits lacking their …
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Analysis of the multiple chaperonins of Mycobacterium smegmatis
… a single gene for the essential oligomeric chaperonin Cpn60, many contain two or more cpn60 genes. The non-pathogenic Mycobacterium smegmatis has three cpn60 homologues, while the pathogenic Mycobacterium tuberculosis has two. This study is a functional characterisation of the chaperonins of …
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Allostery and GroEL: Exploring the Tenets of Nested Cooperativity
… on the functional cycle of the <i>E. coli</i> chaperonins GroEL and GroES, no model proposed to date accounts for all the effects seen experimentally by the various allosteric ligands: ATP, ADP, SP, GroES, and K+. The work in this dissertation explores the various allosteric transitions in the …
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No-Independent Modulation of Soluble Guanylyl Cyclase (Sgc) Activity and Function
… while interaction of sGC with the η subunit of chaperonin-containing T-complex polypeptide 1 (CCTη) or with protein disulfide isomerase (PDI) decreases NO-stimulated cGMP production. Previous experiments demonstrated that the G-protein signaling modulator protein, activator of G-protein …
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Structural biology of GroEL assisted protein folding
… This chapter ends with a discussion on how chaperonin machine like GroEL promotes the correct folding of various proteins.
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Comparison of wild-type and hotspot mutant p53 interactomes: The hunt for mutant-specific binding partners & Investigation and characterization of natural killer cell responses in genetically-engineered mouse models of non-small cell lung cancer
… increased interaction between p53 R172H and the chaperonin CCT8. Further investigation to validate mutant-specific and pan-mutant binding partners found in this study could unlock a plethora of potential new treatments for cancers harboring p53 point mutants. Part 2: While there has long been …
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Analogues of Nitrofuran Antibiotics are Potent GroEL/ES Pro-drug Inhibitors with Efficacy against Enterococcus Faecium, Staphylococcus Aureus, and Escherichia Coli
… to bacterial survival. The bacterial GroEL/ES chaperonin system is viewed as a viable antibiotic target. It has been proven essential to bacterial survival and homeostasis under all conditions. Previous studies identified hit GroEL/ES inhibitors with potential antibiotic activity. One promising …
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Smooth Muscle Cell Stress Response: Essential in Atherosclerotic Pathogenesis
… is misfolded and retained in the cytoplasmic chaperonin containing T-complex polypeptide-1 folding complex in <em>Acta2<sup>R149C/+</sup></em> SMCs. This retention activates heat shock factor 1, which drives endogenous cholesterol synthesis through increased HMG CoA reductase levels, leading …
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Characterizing the genotypic and phenotypic diversity of Gardnerella vaginalis from vaginal clinical samples
… isolates were successfully genotyped using their chaperonin-60 (cpn60) sequences, revealing the presence of four phylogenetic clades (subgroups A-D) made up of 13 subgroup A, 17 subgroup B, 58 subgroup C and 2 subgroup D isolates. To determine the phenotypic diversity of G. vaginalis isolates, …
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Investigation of Interaction between heat shock protein 70.14 (Hsp 70.14) and RBBP6 RING finger domain
… Studies have shown that tumour cells require Hsp chaperonin activities for survival and proliferation than normal cells, because most oncoproteins in invading cells are often unfolded, therefore require chaperonage protection of Hsps for survival. Hsp70.14, alternatively known as HSP70L1, is an …
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Microtubules, Acetylation, and MEC-3 Regulated Genes in C. Elegans Mechanosensations
… development and function and identifying the CCT chaperonin complex as being needed for TRN function. LIM-HD transcription factors play important developmental roles across phyla particularly in neurons. The data presented in this thesis provide insight into LIM-HD function in general and provide …
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