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Showing 1 to 20 of 43 for “"cfDNA"”.
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Patient Preferences for Ultrasound Soft Sign Disclosure with Prior Negative cfDNA Screening
… In the age of noninvasive cell-free DNA (cfDNA) prenatal screening, which exhibits superior sensitivity and specificity for aneuploidy compared to what can be provided by soft sign risk adjustment, the utility of these soft signs is arguably waning. Thus, this study aimed to establish …
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Determination of the Feasibility of Using Cell-Free DNA (cfDNA) in the Q-Clamp KRAS Codon Specific Mutation Test
… was performed using cell-free extracted DNA (cfDNA) instead of FFPE extracted DNA. The results of the experiment showed a clear difference in the Ct’s between the mutant and internal control. Since the delta Ct’s can be discriminated, the assay showed a positive feasibility of using a cfDNA in …
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Biological, Clinical, and Functional Implications of Cell-Free DNA Topology in Cancer
… of cell death. In oncology, this cell-free DNA (cfDNA) has emerged as a promising source of cancer biomarkers because it contains actionable information about a patient’s disease and can be readily obtained via a simple blood draw. In addition to its use in biomarker applications, cfDNA may also …
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Investigating the role of circulating cell-free dna as a mechanistic biomarker in inflammatory bowel disease: development of an integrated precision-medicine enabled platform in Scotland
BACKGROUND: Circulating cell-free DNA (cfDNA) represents a class of biological molecules whose role in inflammation remains poorly understood. Inflammatory bowel disease (IBD), from ulcerative colitis to Crohn’s disease, comprises a spectrum of chronic immune-mediated conditions with complex …
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Dynamics and utility of cell-free DNA release following radiotherapy in prostate cancer and non-small cell lung cancer
… The tissue-of-origin of plasma cell-free DNA (cfDNA) can be determined by methylation analysis and circulating tumour DNA identified by next-generation sequencing (NGS). To evaluate the dynamics of cfDNA during radical radiotherapy, total cfDNA was quantified in patients undergoing radiotherapy …
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Die Rolle der zellfreien DNA in der Insulinresistenz
… dass es einen positiven Zusammenhang zwischen cfDNA und Insulinresistenz gibt, bei dem über cfDNA Verbindungen zwischen Adipositas, einer Subinflammation im (Fett-) Gewebe und Insulinresistenz besteht. Durch die pathologische Dauerstimulation der Insulinrezeptoren entsteht eine …
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Qualify Microplate Reader’s Specification and Establish the Acceptance Criteria for Modified PicoGreen Kit in Measuring cell-free DNA Concentration in Clinical Laboratory
<p>Cell-free DNA (cfDNA) is an emerging biomarker that researchers are utilizing to detect diseases in patients, allowing physicians to accurately diagnose and treat patients at the individual level. A few applications that utilize cfDNA to detect diseases in IVD (in-vitro diagnostics) are …
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A Method for Identification of Pancreatic Cancer through Methylation Signatures in Cell-Free DNA
… body. Evaluation of circulating cell-free DNA (cfDNA), specifically, detection of circulating tumor DNA (ctDNA), is being explored as an approach for non-invasive ‘liquid biopsy’ that can be deployed widely and cost-effectively to screen for early signs of disease. DNA methylation signatures …
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Detecting tumour signals from multi-modal biological features of cell-free DNA fragments
… genome sequencing (sWGS) data of cell-free DNA (cfDNA) isolated from plasma. cfDNAs in body fluids inform non-invasive cancer detection. Multimodal Artificial Intelligence (AI) can improve sensitivity by exploiting various biomarkers when cancer signal is sparse. Tumour-informed assays depending …
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Exploration of cell-free DNA’s biological properties for better understanding and improved circulating tumour DNA detection
… can be used for cancer profiling. Cell-free DNA (cfDNA) are fragmented DNA shed into circulation from various types of cells. The cfDNA derived from cancer cells, circulating tumour DNA (ctDNA), forms a subset of cfDNA. It can be challenging to detect ctDNA as it often constitutes only a minor …
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Expanding the accuracy, resolution, and breadth of cell-free DNA investigation
… identities in the form of cell-free DNA (cfDNA), or the scant amount of highly fragmented DNA circulating in human plasma. As the detritus of apoptotic and necrotic cell death in multiple tissues throughout the body, this class of molecule serves as a powerful biomarker for noninvasive …
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Unleashing the potential of liquid biopsy: allele-informed evaluation of plasma samples for cancer patients management
… and next-generation sequencing of cell-free DNA (cfDNA) in cancer patients’ plasma offer a minimally-invasive solution to detect tumor cell genomic information to aid real-time clinical decision-making. Reliability and sensitivity in the detection of genomic alterations is crucial for patient …
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Detection of Cell-free Tumor DNA in Liquid Biopsies of Dogs with B cell Lymphoma: A Biomarker Discovery
… collected included: (1) blood for plasma (cfDNA), (2) tumor tissue fine-needle aspirates (tumor DNA), and (3) buccal swabs (genomic DNA, germline control). Whole Genome Sequencing was performed using Illumina NovaSeq 6000, and the sequenced output was analyzed with bioinformatics tools to …
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Towards a blood-based biomarker for remyelination: Glial cell death and associated byproducts in demyelinating disease
… Recent studies have found that cell-free DNA (cfDNA) released from dying neurons and glia can be detected in the bloodstream using cell-specific methylation patterns. A better understanding of remyelination-associated glial cell turnover dynamics may aid in the discovery of relevant liquid …
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Cell-free DNA and tumor exosome cargo as diagnostic and prognostic marker for prostate cancer
… was aimed to characterize plasma and urinary cfDNA and tumour exosome cargo as diagnostic biomarker for PCa in South African populations with the goal of discovery of reliable, non-invasive, and novel biomarkers of PCa. We performed miRNA sequencing of exosomal RNA extracted from high and low …
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Surguvant inflammation and metabolism in colorectal surgery
… perioperative body composition, cell free DNA (cfDNA) and metabolomic data and apply these as prognostic markers and potential therapeutic targets in the treatment of colon cancer. Hypothesis: Perioperative non-invasive methods of predicting clinical and cancer outcomes may be adopted in colon …
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Know Thy Cell-Free DNA: Early Detection of Microsatellite Instability Using Ultra-Low-Pass Cell-Free DNA Sequences
… from true mutations. Similarly, cell-free DNA (cfDNA) are DNA fragments from cells all over the body, which circulate in the blood. Collecting and sequencing cfDNA is much less invasive than collecting tissue samples, but presents another challenge in that the fraction of DNA fragments from any …
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Exploring the effect of peri-operative inflammation on systemic and local breast cancer biomarkers
… the perioperative dynamics of cell free DNA (cfDNA) in breast cancer and explore its prognostic potential. 3. To determine the perioperative dynamics of circulating tumour DNA (ctDNA) in breast cancer and define its prognostic potential. Methods This study is a sub-study of the ICORG 09-07 …
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The Influence of Scientific, Genetic, and Health Literacy on Carrier Screening Decision-Making
… X-linked conditions, as well as cell-free DNA (cfDNA) screening for aneuploidy, are recommended during pregnancy. Despite similarities in purpose, sample requirements, insurance coverage, and safety, a lower percentage of individuals elect carrier screening than cfDNA screening, suggesting there …
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Next Generation Sequencing nell'analisi del DNA fetale da plasma materno per la diagnosi prenatale non invasiva di malattie genetiche
… provided a robust method to detect and analyze cfDNA (cell free DNA) from maternal plasma. Our intent is develop new protocols able to carry forward a noninvasive diagnosis starting from cffDNA. In this work, we propose a protocol that allow fetal genotype detection from ccfDNA through a target …
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