Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"cerebral organoids"”.
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Elucidating Astrocytic Diversity in Human Cerebral Organoids
… newfound astrocytic diversity in human cortical organoids grown at the air-liquid interface (ALI-COs) for up to 495 days in vitro (DIV). It shows that ALI-CO development displays considerable similarities with the human fetal cortex and undergoes significant gliogenesis at 200DIV. In particular, …
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Cerebral Organoids as 3-Dimensional Grafts in Traumatic Brain Injury
… recent emergence has led researchers to examine organoids’ potential as tissue grafts to regenerate the lost tissues in the brain following traumatic brain injury. In this dissertation, we sought to understand the interaction between the organoid and host following transplantation as a tissue …
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INVESTIGATING THE ROLE OF FEZ1 IN EARLY HUMAN BRAIN DEVELOPMENT USING CEREBRAL ORGANOIDS
… of FEZ1 during early brain development by human cerebral organoids model and loss of function study. Transcriptome analysis revealed genes associated with neuronal development and synaptic function were differentially expressed in FEZ1 knockout organoids, suggesting FEZ1 participated in early …
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Modelling Prenatal Hypoxia As A Risk Factor For Schizophrenia Vulnerability In Patient-Derived Cerebral Organoids
… unclear. Our laboratory has developed human cerebral organoids using induced pluripotent stem cells (iPSCs) derived from healthy control or SCZ patient cell lines to address these questions. This creates a platform that allows for the investigation into the pathophysiology of SCZ and hypoxia …
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Scaling up 3D imaging, analysis, and culture of complex brain models
… intact mouse beams, marmoset bi am samples, and cerebral organoids The mouse brain pipeline allows region-based statistical analysis of protein expression and cell counts An efficient single-cell non-rigid coregistration algorithm for multiplexed volumetric fluorescence imaging based on matching …
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Neocortical functional network development at the microscale: impairments and mechanisms in Rett Syndrome.
… a graph theoretical approach in 3D, human tissue—cerebral organoids—for future research to study neocortical network development in health and disease. Micro-electrode array recordings of primary murine cortical cultures showed that Mecp2-deficient, Rett Syndrome model networks showed impairments …
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Advanced Bioelectronic and Microphysiological Systems for Functional Studies of Stem Cell-Derived Neural Models
… bioelectronic systems; • Air-liquid interface cerebral organoids (ALI-COs), interfaced with Neuroweb, a porous, ultra-flexible microelectrode array enabling chronic electrophysiology; • NeuroMaps, a modular MATLAB-based GUI for multimodal electrophysiological analysis, integrating spike …
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Investigating the role of ZEB2 in the establishment of neuroepithelial architecture and axon tract formation
… In this work we show that in both human brain organoids and mouse embryos ZEB2 is expressed in telencephalic neuroepithelial cells (NECs) before the switch to radial glia (RGCs). By establishing a human embryonic stem cell (hESC) model of ZEB2 heterozygous loss-of-function we show that this …
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SPINAL MUSCULAR ATROPHY ORGANOIDS REVEAL DEVELOPMENTAL DEFECTS RESCUED BY ANTISENSE OLIGONUCLEOTIDES TREATMENT
… In the present study, we generated SMA type 1 cerebral and spinal cord organoids (SCOs) to recapitulate human-specific early neurodevelopmental features. Single-cell transcriptomics revealed a pervasive transcriptional alteration in multiple cell populations, along with drastic changes in motor …
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Interaction between form and fate during human neural development
… and fate during neural development. We relied on cerebral organoids as a tractable *in vitro* system where tissue shape can be manipulated following several complementary approaches and tissue identity assessed by transcriptomics. We first discovered key protocol variables that contribute to …
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Investigating the effects of tau mutations in induced pluripotent stem cell-derived neurons
… patient-derived P301L MAPT-mutant iPSCs, I made cerebral organoids (COs). Similar amounts of neuronal, astrocyte, and synaptic proteins were detected in the control and MAPT-mutant COs, as were cortical layer markers indicating a forebrain-like fate. Phosphorylation at the AT8 epitope was …
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Integrative and Scalable Pipeline for Multi-omic Characterization of Biological Tissues
… postmortem humans, and three-dimensional (3D) organoids derived from human induced pluripotent stem cells (hiPSCs), can be utilized. Regardless of the models used, a large-scale, unbiased study of the brain is yet to be achieved due to the vast complexity and high interconnectedness of the …
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Astrocytic Sonic Hedgehog signalling in development and disease
… modified rodent models, alongside human-derived cerebral organoids. This research seeks to elucidate the mechanisms by which Shh signalling instructs astrocyte function, heterogeneity, and their contribution to the broader neural environment. My overarching hypothesis is Shh signalling …
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Characterization of A Novel Oncogenic Mirna Inhibitor In Cancer Therapy
… vitro</em>. This effect is also shown in a cerebral organoid model. Using a multidisciplinary approach, this study identifies a relationship between oncogenic miR-10b and the dysregulation of the PI3K/AKT pathway in cancer, along with putting forth a compound that can mitigate these effects. …