Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"centrosomal protein"”.
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Centrosomin Self-Assembly and Centrosomal Protein Recruitment
… entry into mitosis. Centrosomin (Cnn) is a major centrosomal protein that is important for nucleation and organization of bipolar spindle microtubules at mitosis. Cnn protein localizes to the pericentriolar matrix and from there other centrosomal proteins 'load' onto the centrosome, including …
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Functional characterization of the novel centrosomal protein Nlp (ninein-like protein)
… identification and characterization of a novel centrosomal component, the human protein Nlp (ninein-like protein) related to the previously characterized MT-anchoring protein ninein. In the first part of the present thesis we describe the identification of Nlp as a novel centrosomal substrate of …
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Molecular and functional analysis of the tumour antigen T21
… member of cancer/testis antigen (CTAs) family of proteins using a modified SEREX technique and can be considered a promising prostate-associated tumour antigen, shown to elicit a humoral immune response in prostate cancer patients. T21 has also been shown to be over-expressed in malignant glands …
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The physiological role of the two isoforms of DNA topoisomerase II in human cells
… IIbeta does not adopt these functions. A centrosomal protein was recognized by topoisomerase IIalpha. This topoisomerase IIalpha-like protein resembles a modified form of topoisomerase IIalpha with an apparent size of 205 kDa compared to 170 kDa. The expression of the protein is constant …
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Molecular expression and immunotherapeutic potential of the novel tumour associated antigen T21
… SEREX expression cloning showing restricted protein expression to normal testis and prostate, breast, kidney, ovary, melanoma, colon and stomach cancer among others. More recently, T21 was found to share significant sequence similarity with a centrosomal protein called CEP290. This study …
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The PLK1 and NLRP3 interactome in NLRP3 inflammasome activation
… domains responsible for this interaction. Protein-protein interactions regulate protein functionality of both PLK1 and NLRP3. Therefore, I set out to identify the PLK1 and NLRP3 interactome during NLRP3 inflammasome activation, which could indicate the underlying mechanism of how PLK1 …
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Investigating the Biological Functions of the Protein Kinase WNK1 in the Regulation of Cytoskeletal Structures and Membrane Trafficking
… microtubule organization center was observed. Centrosomal structure was impaired. Cells showed a migratory defect. Clues from a former student and my mass spectrometry data suggested that dynein and its associated protein-dynactin, centrosomal protein of 70 and 170 kDa might be potential …
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The Role of CDCA7 in Akt-mediated Myc-Dependent Apoptosis and Proliferation
CDCA7, or cell division cycle associated protein A7, was described in 2001 by Prescott and colleagues as a target of Myc-dependent transcriptional regulation (Prescott et al., 2001). We have identified CDCA7 as associating with the transcription factor Myc and is the target of phosphorylation by …
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Regulation of Mitotic Spindle Assembly in Caenorhabditis elegans Embryos
… regulator of spindle assembly 1), a putative Protein Phosphatase 2A (PP2A) regulatory subunit whose silencing causes defects in spindle formation. Upon rsa-1(RNAi), spindle poles collapse onto each other and microtubule amounts are strongly reduced. My thesis work demonstrates that RSA-1 …
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CHARACTERISATION OF A NOVEL SPINDLE DOMAIN IN MAMMALIAN MEIOSIS
… the absence of centrosomes, a large number of centrosomal proteins are highly expressed in mouse oocytes. Here, I characterise the localisation and function of centrosomal proteins at a previously undescribed meiotic spindle pole domain (MSPD). An initial protein screen identified a group of …
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Identification of centrosomal proteomes using COMPACT, a novel tool for purification of centrosomes
… by appendage structures, and surrounded by a proteinaceous matrix known as the pericentriolar material. Despite a number of advances in the field, focussing particularly on centrosome structure and assembly in normal and diseased cells, relatively little is known about the spatial and temporal …