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Showing 1 to 19 of 19 for “"cellulose hydrolysis"”.
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Characterisation of recombinant beta-glucosidases from Thermomyces lanuginosus SSBP and investigation of their synergistic potential in cellulose hydrolysis
Submitted in complete fulfilment for the Degree of Master of Applied Science in Biotechnology in the Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa, 2020.
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Lignocellulose Saccharification via Cellulose Solvent Based Fractionation Followed by Enzymatic Hydrolysis: the Last Obstacle to Integrated Biorefineries
… structure of lignocellulosic biomass. Cellulose- and organic-solvent-based lignocelluloses fractionation (COSLIF) technology was employed to overcome biomass recalcitrance. Surface response methodology (SRM) showed that optimal COSLIF pretreatment conditions were 85% (w/v) H₃PO₄ and ~50 …
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Cellulase production by fermentation with trichoderma viride on cellulose and wood wastes
… viride QM 6a and the use of this cellulase for cellulose hydrolysis were studied using Solka Floc cellulose and waste wood (oak) as substrates. The fermentation produced higher enzyme concentration solutions with media containing additional amounts of soy peptone and …
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Investigating biomass saccharification for the production of cellulosic ethanol
… biomass pretreatment followed by enzymatic hydrolysis is among the most costly steps for emerging cellulosic ethanol biorefineries. In this project, two pretreatment methods (dilute acid, DA, and cellulose solvent- and organic solvent-lignocellulose fractionation, COSLIF) for corn stover …
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A stereoelectronic and thermodynamic study of b-D-methyl glucose conformational changes related to anomeric centre reactivity
… glucose in glycosidase reactions (for example, cellulose hydrolysis). The investigation is computational and is done in isolation from the enzyme binding pocket and incoming nucleophile. What is the effect of the C1-O1 bond breaking process on key glucose properties is the central question asked …
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Characterization of novel genetic alleles of cellulose synthase
Cellulose is the most abundant polymer on earth. Despite this, relatively little is known about the proteins and biochemical mechanisms governing cellulose biosynthesis. Only in the past 10 years since the sequencing of the Arabidopsis thaliana genome have we begun to unravel the full complexity of …
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Application of the extended Kalman filter to enzyme reactions
… The first model problem is a simulation of cellulose hydrolysis with enzyme inactivation. The second model problem is a simulation of the separation of a chiral substance into its respective enantiomers. In the first problem, the hydrolysis of cellulose, a slowly varying parameter is tracked …
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Cellulolytic enzyme systems of Myrothecium verrucaria
… (B) the formation of glucose in the course of cellulose hydrolysis, (C) the significance of intermediate dextrins in the course of cellulose hydrolysis, (D) the properties of a β-aryl-glucosidase present in filtrates of this organism, and (E) a transglucosidase which synthesized higher …
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Genetic Improvement of Switchgrass Cell Wall Content, Leaf Angle and Flowering Time
… transgenic biomass had significantly increased cellulose hydrolysis (saccharification) efficiency for biofuel production. Erect leaf is a desirable trait to adjust the overall plant architecture to perceive more solar energy and thereby to increase the plant biomass production in a field …
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Enzyme diffusion and cellulose breakdown in the bioremediation of medical waste
… within the waste stream and the breakdown of cellulose-derived material by cellulase enzymes. It was found that the waste stream contained a variety of small crevices which could possibly contain pathogens. Circulation in these crevices was restricted by their small openings and one must rely …
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Cellulose mineralization in two-stage anaerobic digestion systems
… high-strength solid waste/sludge, particulate cellulose, by two-stage anaerobic digestion systems. Cellulose hydrolysis and methanogesis were examined in both the thermo-meso and acid-methane two-stage systems under different hydraulic retention times (HRTs), feed concentrations and organic …
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Compounds Released from Biomass Deconstruction: Understanding Their Effect on Cellulose Enzyme Hydrolysis and Their Biological Activity
… and exoglucanase was determined. The initial hydrolysis rate of cellobiose by betaglucosidase was significantly reduced by the CPC sugar-rich fraction; however, exoglucanase was deactivated by the CPC phenolic-rich fraction. Finally, biological activities of water-extracted compounds from …
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Catalytic conversion of biomass-derived molecules into mono- and dicarboxylic acids and esters
… to enable the sequential one-pot combination of cellulose hydrolysis and glucose oxidation. The study provides insights into the deactivation of the catalysts caused by leaching and hydrothermal sintering of Au nanoparticles, as well as by adsorption of reaction species. We found that lowering …
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Structure and Property of Polymers and Biopolymers from Molecular Dynamic Simulations
… chains grafted on a substrate for biomass hydrolysis was successfully synthesized. A poly (styrene sulfonic acid) (PSSA) polymer chain is immobilized on a substrate and used to catalyze biomass hydrolysis. A neighboring poly (vinyl imidazolium chloride) ionic liquid (PIL) polymer chain is …
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Cell surface display in biomedical applications and biofuels production
… to a more widespread utilization of lignocellulose is the absence of low-cost technology to break down its major component – cellulose. Consolidated bioprocessing (CBP), which combines enzyme production, cellulose hydrolysis, and fermentation in a single step, has been proposed to …
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Upgrading biomass by hydrothermal and chemical conditioning
… higher than water under ambient condition. Hemicellulose hydrolysis occurs at temperatures as low as 180 °C, while cellulose hydrolysis starts around 220-230 °C. Many monomers, aldehydes, and intermediates are produced as a result of hydrolysis of biomass. Reactive intermediate species promote …
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… of thermostable enzymes with low-cost. The hydrolysis performance of 21 reconstituted bacterial cellulase mixtures containing the glycoside hydrolase family 5 Bacillus subtilis endoglucanase, family 9 Clostridium phytofermentans processive endoglucanase, and family 48 Clostridium …
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Pengaruh variasi jumlah ragi roti (Saccharomyces cerevisiae) dan lama fermentasi glukosa hasil hidrolisis selulosa limbah bagas tebu dengan enzim kasar dari campuran kapang Trichoderma sp, Gliocladium sp, dan Botrytis sp terhadap kadar etanol
… that be kibbled. Robust of sugarcane contains cellulose, hemicellulose, and lignin. In this research, hydrolysis process on biotenol making used enzyme from mold,i.e. mixture of Trichoderma sp, Gliocladium sp, and Botrytis sp because it can produce selulase enzyme with a ratio of clear zone to …
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Cellulose photonics: designing functionality and optical appearance of natural materials
Cellulose is the most abundant biopolymer on Earth as it is found in every plant cell wall; therefore, it represents one of the most promising natural resources for the fabrication of sustainable materials. In plants, cellulose is mainly used for structural integrity, however, some species organise …