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Showing 1 to 20 of 37 for “"cellulases"”.
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Possibilities for recycling cellulases after use in cotton processing
… activity (measured as fabric weight loss) of the cellulases remaining in solution, however, decreased by 80% over the five cycles. It was suggested that this could be due to end-product inhibition, thermal and or mechanical deactivation, or to the loss of some components of the cellulase complex …
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Molecular Ecology and Evolution of Fibrobacter and Its Cellulases
Fibrobacter is a major genus of fiber digesting bacteria of the bovine rumen. It is the only Gram ($-$) anaerobic cellulolytic bacteria identified at present. Strains of Fibrobacter are indistinguishable from each other using traditional phenotypic criteria. Of 21 substrates examined, only …
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Effects of the Non-ionic Surfactant Tween 80 on the Enzymatic Hydrolysis of Model Cellulose and Lignocellulosic Substrates
… different sulfate group densities. Adsorption of cellulases onto sulfated substrates increased with increasing sulfate group density but their rate of hydrolysis decreased. The decrease indicated an inhibitory effect of sulfate groups on the enzymatic hydrolysis of cellulose, possibly due to …
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Enzyme diffusion and cellulose breakdown in the bioremediation of medical waste
… This biological process also utilizes cellulases to breakdown the paper within the waste stream. Small-scale simulated waste experiments were conducted with cellulases to determine the increase in maximum mixable solids concentration and the mass reduction of the waste due to cellulase …
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Cellulases from extremely thermophilic anaerobic bacteria: a comparison of several new cellulolytic isolates and the partial purification and characterisation of components of the cellulase complex from one isolate
Forty-seven thermal pool sites in the central volcanic plateau of the North Island of New Zealand were sampled and enrichment cultures on crystalline cellulose screened for growth and cellulase activity. Eight anaerobic cellulolytic cultures resulted. Cellulolytic isolates were obtained from five …
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ΜΕΛΕΤΗ ΤΟΥ ΜΗΧΑΝΙΣΜΟΥ ΠΡΟΚΑΤΕΡΓΑΣΙΑΣ ΓΙΑ ΤΗΝ ΜΕΤΑΤΡΟΠΗ ΤΗΣ ΒΙΟΜΑΖΑΣ ΣΕ ΚΑΥΣΙΜΑ ΚΑΙ ΧΗΜΙΚΑ: ΕΠΙΔΡΑΣΗ ΣΤΗ ΣΤΕΡΕΑ ΦΑΣΗ
… NIGER, TRICHODERMA VIVIDE AND FUSARIUM OXYSPORUM CELLULASES AND ON BIOETHANOL PRODUCTION WITH F . OXYSPORUM CELLULASES AND ON BIOETHANOL PRODUCTION WITH F. OXYSPORUM WAS EVALUATED D.CONVERSION YIELDS ARE INVERSE LINEAR FUNCTIONS OF PARTICLE SIZE AND LINEAR FUNCTION OF DEGREE OF DELIGNIFICATION. …
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Pure culture and metagenomic approaches to investigate cellulose and xylan degradation
… for the use of lignocellulose. The study of cellulases and xylanases that degrade cellulose and xylan into constituent monosaccharides is required to advance industrial application of these enzymes. The use of a traditional pure culture approach to discover and characterize cellulases and …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
… CelD polypeptide had common features with other cellulases e.g. a putative catalytic domain, hydroxyamino acid rich domain, a putative cellulose binding domain, and repeated amino acid sequences near the C-terminus. In addition, CelD showed amino acid sequence similarity with other Ruminococcus …
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Molecular Design and Mechanistic Characterization of Glycoside Hydrolases using Computational and Experimental Techniques
… activity and stability of enzymes. So obtaining cellulases with high thermostability and simultaneously enhanced activity is a great challenge in the field of bioethanol production. In the studies presented in this dissertation, different computational techniques, such as Molecular Dynamics (MD), …
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Spatial variability of soil biological and chemical indicators in selected soils in South Africa
… Spatial dependence of the soil respiration, cellulases and organic carbon have strong spatial dependence with nugget to sill ratios of less than 25% in the field of University of Venda Experimental Farm. Thus, catalase, acid phosphatase and organic phosphorus exhibited moderate spatial …
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Characterization of endoglucanase cela from the rumen bacterium Clostridium longisporum
… source of renewable energy and other chemicals. Cellulases are being studied to elucidate the enzymatic degradation of cellulose. We are interested in the molecular mechanisms of microbial degradation of cellulose in the rumen. The long-term aim is the potential genetic modification of …
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EVALUATION OF CELLULOLYTIC ENZYMES FROM A NEWLY ISOLATED BREVIBACILLUS SP. JXL; AND OPTIMIZATION OF COSLIF PRETREATMENT VARIABLES OF SWEET SORGHUM BAGASSE USING A RESPONSE SURFACE METHOD
… aeration, strain JXL produced a basal level of cellulases as FPU of 0.02 IU/ml in the crude culture supernatant. When glucose or cellobiose was used besides cellulose, cellulase activities were enhanced ten times during the first 24 h, but with no significant difference between the effects …
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The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen
… focused on the poorly characterised exo-acting cellulases from rumen bacteria. Preliminary enzymology studies on one cellulase from Ruminococcus flavefaciens FD-1, previously isolated in this laboratory, indicated that an exo-acting cellodextrinase, CelA, had been isolated. In this report, the …
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The Termite Digestome: Understanding the Digestive Physiology Involved in Lignocellulosic Biomass Degradation
… saccharification in the presence of recombinant cellulases. These results suggest that utilizing Reticulitermes flavipes as a source of feedstock-specific recombinant enzymes for utilization in commercial biorefinery processes can likely decrease biocatalyst input while increasing simple sugar …
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Characterization of Cellulase Enzyme Inhibitors Formed During the Chemical Pretreatments of Rice Straw
… hydrolyzates. Elucidating the inhibition of cellulases using actual biomass hydrolyzates would offer insights as to which inhibitors, formed during a pretreatment, are key in causing inhibition. Knowing the key inhibitor(s) would allow for the development of processing conditions that …
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Recombinant production and regioselectivity study of lytic polysaccharide monooxygenases
… making it better accessible for classical cellulases. Therefore, these enzymes are currently attracting much scientific attention. Although they all have a relatively similar active site, the target of their oxidative activity is known to differ amongst LPMOs. In this thesis, an extensive …
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Celulases para produção de etanol de segunda geração
… the use of cellulolytic enzymes. The potential cellulases assume that the emerging industry bioenergy and bioproducts becomes a motivation for the development of improved enzyme preparations for hydrolysis of cellulose. However, a major difficulty in the implementation of a biomass bioconversion …
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Molecular mechanisms behind the adjustment of phototrophic light-harvesting and mixotrophic utilization of cellulosic carbon sources in Chlamydomonas reinhardtii
… was shown to be performed by extracellular cellulases CrCel9B and CrCel9C that belong to glycoside hydrolase family 9 (GHF9/subgroup E2). These enzymes display homology to cellulases from Metazoa and phylogenetic analyses suggested that they originated from an ancient eukaryotic ancestor. …
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Improving Brassica napus straw for cellulosic ethanol production
… pretreatments are effective, but commercial cellulases are poorly adapted to hydrolyse Brassica napus straw. Following pretreatment, saccharification was limited by uronic acids and xylose retention, which impeded cellulase performance. Surprisingly, lignin abundance correlated positively …
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Biological wastes as plant growth substrate amendments
… plant defense proteins, namely chitinases and cellulases. Protective effects were seen in all crop models but the results indicate that further trials are required to confirm long-term efficacy. The second part of the research investigated the persistence of enteric bacteria in raw salad …
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