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Showing 1 to 20 of 122 for “"cellulase"”.
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Investigation of Ruminococcus flavefaciens FD-1 cellulase
Restriction data tranferred 2014-07-01T11:23:52-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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Regulation of cellulase activity and synthesis in Clostridium thermocellum
Thesis (Sc.D.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1984.
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Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers
… surfactant Tween 80 on the adsorption of cellulase onto alkane thiol self-assembled monolayers (SAMs) on gold was also studied. Methyl (-CH3), hydroxyl (-OH) and carboxyl (-COOH) terminated SAMs were prepared. Adsorption of cellulase onto untreated and Tween 80-treated SAMs were monitored …
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Cellulase and related enzyme activity in protoplasts and mycelium of filamentous fungi.
Production of cellulase, pectinase and xylanase was investigated using culture filtrates from selected mesophilic (Aspergillus niger, Penicillium funiculosum, Penicillium janthinellum, Penicillium ochrochloron, Trichoderma viride), thermotolerant (Aspergillus fumigatus) and thermophilic (Geosmithia …
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Cellulase production by fermentation with trichoderma viride on cellulose and wood wastes
<p>"Cellulase production by the fungus Trichoderma viride QM 6a and the use of this cellulase for cellulose hydrolysis were studied using Solka Floc cellulose and waste wood (oak) as substrates. The fermentation produced higher enzyme concentration solutions with media containing additional amounts …
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Properties of C(x) Cellulase and Cellobiase From Fruits of Lycopersicon Esculentum
Made available in DSpace on 2014-12-12T00:57:21Z (GMT). No. of bitstreams: 1 7212338.pdf: 2443496 bytes, checksum: 2da0d7ca7303e0717130070872f4ce60 (MD5) Previous issue date: 1971
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Biosynthesis and identification of enzymes of the cellulase system of Trichoderma reesei
… activity. Some of the enzymic components of the cellulase system produced by T. reesei under these conditions were purified by ion exchange chromatography on DEAE-Sephadex A-50. Two cellobiohydrolases were isolated in pure form. One of these was identical to the previously isolated …
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The purification of C₁ components from the cellulase complex of Trichoderma viride
… of crystalline cellulose, has been isolated from Cellulase Meiji, a commercial cellulase derived from culture filtrates of Trichoderma viride. Initial purification was accomplished with a substrate affinity column, using Avicel as the crystalline cellulose substrate. The resulting Avicel Water …
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Impact of Amphiphiles in Cellulase Stabilization, Recycling and Ethanol Production from Pretreated Biomass
… as a lignin-blocker for biomass and potential cellulase stabilizer in hydrolysate. A variety of caseins blocking buffers, such as complete commercial casein, freeze-dried acid casein, gluten and lactose-free casein and liquid casein (containing 0.01-0.45% lactose) and ultrafiltered casein were …
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Characterization of Cellulase Enzyme Inhibitors Formed During the Chemical Pretreatments of Rice Straw
… enzyme cocktail is made up of endo-cellulase, exo-cellulase and beta-glucosidase enzymes, whose role is to cleave cellulose polymers into glucose monomers. Specifically, endo-cellulase and exo-cellulase enzymes cleave cellulose chains in the middle and at the end, resulting in …
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Cellulase and Polygalacturonase in Tomato Fruits and the Effect of Calcium on Fruit Cracking
Made available in DSpace on 2014-12-04T19:49:29Z (GMT). No. of bitstreams: 1 6303230.pdf: 2370719 bytes, checksum: 8d459bd504924ff8cd41c5be9fa22b52 (MD5) Previous issue date: 1962
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Molecular analysis of two cellulase genes from Ruminococcus flavefaciens FD-1 and their transcriptional regulation
… a cellodextrinase gene (celA) and a carboxymethylcellulase gene (celE), and study their regulation and induction at the transcriptional level. The sequence of the celA gene from FD-1 was determined and the amino acid sequence of the CelA enzyme (336 amino acid residues) deduced. It showed 40% …
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The importance of crystal lattice structure of cellulose in the production and action of cellulase
… structure on the induction and action of "cellulase.” The study was also aimed to obtain evidence from these experiments which may be helpful in elucidating the mechanism of the degradation of crystalline cellulose. The results of various experiments clearly demonstrated the importance of …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
… and supported the zymogram results that the cellulase and xylanase activities were located on a single polypeptide. DNA sequence analysis of celD revealed an open reading frame of 1086 nucleotides encoding a 362 amino acid polypeptide with a molecular mass of 46,329 daltons. The CelD …
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Structural characterization of a glycoprotein cellulase, β-(1[arrow]4)-glucan cellobiohydrolase C, from Trichoderma viride
… and identical to the "C₁" enzymes, F-II cellulase and hydrocellulase C, purified previously in this laboratory. Purified cellobiohydrolase C has an E₂₈₀<sup>1%</sup> = 14.2and degrades both microcrystalline and phosphoric acid-swollen cellulose to cellobiose at the rates of 213 and 738 …
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Use of the cellulose-binding domain of a cellulase from cellulomonas fimi for affinity purification of fusion preteins
… cellulose-binding domain (CBD) from a bacterial cellulase as an affinity tag for purification of heterologous proteins. The CBD of endoglucanase A (CenA) from Cellulomonas fimi is an N-terminal domain comprising amino acids. CenA binds strongly to cellulose, and the CBD retains this function when …
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… performance of 21 reconstituted bacterial cellulase mixtures containing the glycoside hydrolase family 5 Bacillus subtilis endoglucanase, family 9 Clostridium phytofermentans processive endoglucanase, and family 48 Clostridium phytofermentans cellobiohydrolase was investigated on …
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Investigating biomass saccharification for the production of cellulosic ethanol
… measurements of total substrate accessibility to cellulase (TSAC), cellulose accessibility to cellulase (CAC), and non-cellulose accessibility to cellulase (NCAC) based on adsorption of a non-hydrolytic recombinant protein TGC were established to find out the cause. The COSLIF-pretreated corn …
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Possibilities for recycling cellulases after use in cotton processing
… treatments on cotton fabrics, using a total cellulase from Trichoderma reesei, showed that active enzymes remained in the treatment liquor and adsorbed on the fabric substrates after five treatment cycles. The activity (measured as fabric weight loss) of the cellulases remaining in solution, …
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Increasing dry grind ethanol yield through fiber pretreatment after liquefaction
… of hot water pretreatment, disk milling and cellulase dose on conversion of corn fiber into ethanol and to modify the conventional dry grind process for converting corn fiber into ethanol. Mash obtained after liquefaction was filtered and retentate (referred to as fiber) was used to study …
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