Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 39 for “"cellular transformation"”.
-
MicroRNAs in cellular transformation and tumorigenesis
MicroRNAs (miRNAs) are a novel class of small (approximately 23 nucleotides long), highly conserved, non-coding RNAs that function by broadly regulating gene expression. In animals, this regulation is achieved via interaction with target messenger RNAs (mRNAs), largely through their imperfect base …
-
The role of BCLFA1 in cellular transformation
The malignant transformation of normal cells into cancer cells result in the loss of control of cellular regulatory mechanisms such as loss of function of tumour suppressors and gain of function of oncogenes. Genetic mutations may be inherited or acquired during the process of malignant …
-
The role of cellular micro-RNAs in Epstein-Barr virus induced cellular transformation and oncogenesis
… is known about the contribution of miRNAs to the transformation of B cells. We initially examined the regulation of the oncogenic miR-155, which is highly expressed in Hodgkin’s lymphoma but was reportedly absent in Burkitt’s lymphoma. We found that miR-155 was up-regulated by EBV-LMP1 expression, …
-
THE EFFECTS OF RETINOIC ACID ON CELLULAR TRANSFORMATION AND TUMORIGENESIS INVOLVING CELLS WITH KNOWN ONCOGENES (VITAMIN A, RETINOIDS, RETROVIRUS)
Vitamin A is known to have an important role in cellular differentiation and proliferation. In addition to regulating normal cellular processes vitamin A has also been shown to possess potent antineoplastic activity. The work in this dissertation characterizes the role of retinoic acid (RA) in …
-
The role of DNA methylation in transcriptional regulation of the human type 1 alpha 2 collagen (COL1A2) gene
… correlated with pathological conditions and cellular transformation. Numerous studies have suggested that methylation of the cytosines in CpG dinucleotides is inversely correlated with transcriptional activity and plays a critical role in differential gene expression.
-
Analysis of the function of the Nf2 tumor suppressor protein, Merlin
… actin cytoskeleton. However, the molecular and cellular functions of this tumor suppressor gene were largely unknown when the work described here began. Mutational analysis of Nf2 in flies has lead to the identification of a dominant-negative allele, which harbors mutations in the amino-terminal …
-
Gene mutations and expression in breast cancer
… suppressor genes playing an important role in cellular transformation and ultimately in tumour formation. In this study, 40 breast cancer patients from the Western Cape province in South Africa and 4 breast cancer cell lines were screened for mutations in the human Ha-ras oncogene and the p53 …
-
The role of viral sequences in genetic aberrations and malignant transformation
… the low risk HPV11 and high risk HPV18 on the cellular gene expression profile in order to identify genes required for the initiation of cellular transformation and also to identify genomic alterations associated with oesophageal squamous cell carcinoma. Cancer is a leading cause of death …
-
AGO2 in overexpression exhibits oncogenic functions KrasG̳1̳2̳D̳ -associated mouse tumor models
… focuses on the role of Argonaute-2 in promoting cellular transformation in mouse model systems, highlighting novel oncogenic functions associated with AGO2 overexpression. In short, we have determined that AGO2 overexpression promotes metastasis in an autochthonous mouse model of non-small cell …
-
Use of fourier transform- infrared spectroscopic imaging as a tool for understanding early molecular events driving breast cancer progression
… it is important to study this characteristic cellular transformation in vitro. We report a direct comparison between α-SMA expression in primary normal human dermal fibroblasts and molecular spectra obtained through Fourier Transform infrared (FT-IR) spectroscopic imaging. Fibroblasts were …
-
Methods and Mechanisms of DNA Methylation in Development and Disease
… regulation and is required for normal cellular differentiation. Failure to properly establish or maintain DNA methylation patterns leads to cell dysfunction and diseases such as cancer and neurological disorders. The goal of this thesis is to understand the role of DNA methylation in …
-
Inactivation of the JAK-STAT Pathway
… pathway can lead to abnormal development and cellular transformation demonstrating the importance of understanding mechanisms of downregulation. After interferon-γ (IFN-γ) treatment of cells the appearance of tyrosine phosphorylated Stat1 in the nucleus was maximal within 20-30 minutes, …
-
Using High-Resolution NMR to Examine the Components that Contribute to the Activity and Stability of Phosphatase of Regenerating Liver (PRL-1) and Their Dependence on the Redox State of Cysteine Residues
… cancer tissues and when over-expressed induces cellular transformation. Elevated levels of PRL-1 have been shown to trigger metastasis and to promote cellular transformation and tumorigenesis. Because its enzyme activity contributes to both tumorigenesis and metastasis, this protein is an …
-
Metabolomic Investigation of Melanoma Metastasis in a Patient-Derived Xenograft Mouse Model
… reprogramming is considered a major factor in cellular transformation and tumor initiation, but whether or how metabolism supports tumor metastasis remains an open question. This study seeks to identify metabolic predictors of metastasis, with the rationale that understanding metabolic changes …
-
Characterizing the role of p21-Activated Kinase 3 (PAK3) in AP-1-induced transformation
… and the role of PAK3 in cJun/AP-1-associated cellular transformation. Our results showed elevated PAK3 expression at both the mRNA and protein level in cJun-over-expressing Rat1a fibroblasts, as well as in transformed human fibroblasts. Elevated PAK3 protein levels were also seen in cervical, …
-
Longitudinal Keratinocyte Proliferation Induced by Human Papillomavirus 16E6 and NFX1-123 Partnership
… a significant role in inducing and maintaining cellular transformation of its infected host cell; however, 16E6 itself has no enzymatic activity and carries out most of its functions through partnerships with host endogenous proteins. Previously, it was demonstrated that 16E6 binds to the host …
-
Analysis of the Role of the Transcription Factor-C/ebpbeta in Implantation
… pathways underlying this hormonally induced cellular transformation, an essential prerequisite for embryo implantation, are poorly understood. We previously identified the transcription factor C/EBPbeta as a target of steroid hormone regulation in the uterus. The uteri of C/EBPbeta-null mice …
-
Analysis of the role of the transcription factor-C/EBPβ in implantation
… pathways underlying this hormonally induced cellular transformation, an essential prerequisite for embryo implantation, are poorly understood. We previously identified the transcription factor C/EBPβ as a target of steroid hormone regulation in the uterus. The uteri of C/EBPβ–null mice failed …
-
Investigating the initiation and progression of small cell lung cancer
… revealed cooperation between Nfib and Lmyc in cellular transformation. Comparative genomics identified NFIB amplifications in human SCLC and uncovered a role for NFIB in regulating cell viability and proliferation. We also examined the effect of lung injury on SCLC initiation and progression …
-
Higher order chromatin degradation induced by hydrogen peroxide in glial cells
… concentrations as low as 50 muM. Chelating intracellular calcium with 1,2-bis( o-aminophenoxy)ethane-N,N,N',N' -tetraacetic acid tetra(acetoxymethyl) ester (BAPTA/AM) prior to H 2O2 exposure inhibited HOCD in a dose dependent manner. However, neither removing extracellular calcium, nor blocking …
Page 1 of 2