Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"cellular localisation"”.
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Investigating the cellular localisation of DEAD box helicase DDX3
… We were also interested as to whether DDX3's cellular localisation was regulated during viral infection, cellular stress and during the cell cycle. We investigated the relationship of DDX3 with HIV and HCV viral proteins, and found that both HIV and HCV target the cellular localisation of …
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Biochemical investigation of phosphodiesterase type IV post-translational modification, cellular localisation and interaction with associated binding proteins
… regions, which play a crucial role in sub-cellular localisation and protein-protein interactions. It is shown here that PDE4D5 interacts with a novel RhoGAP called ARHGAP21 which has been previously reported to bind β-arrestins. This interaction is independent of GAP activity of ARHGAP as …
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The role of the E2 copper binding domain in the cell biology of the amyloid precursor protein
… copper binding domain (E2 CuBD) within the extracellular region of the protein. The current project aims were to investigate how copper binding within the APP E2 CuBD might influence the expression, proteolysis, cellular localisation and cytotoxicity of APP. In order to achieve this, a range of …
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Studies of the PTEN tumour suppressor in endometrial cancer.
… PIP3, this enzyme modulates several cellular functions e.g. proliferation, differentiation and migration.The PTEN protein comprises a 403 amino acid, 55KDa protein which is present in the cytoplasm but has also been detected in the nucleus of some cells. The function of nuclear versus …
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Characterisation and functional analysis of a novel msp domain – containing protein, MOSPD1
… Mospd 1 and Mospd 3 by identifying the sub-cellular localisation of MOSPD1 and MOSPD3 in both cells and tissues and to investigate the function of MOSPD1. Mouse monoclonal antibodies specific for MOSPD1 and MOSPD3 were generated and tested to ensure they did not cross react. MOSPD1 was found …
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Molecular Pathogenesis of MALT lymphoma
… and 4394 additional gene sets covering various cellular pathways, biological processes and molecular functions showed that translocation-positive MALT lymphomas were characterized by an enhanced expression of NF-κB target genes, particularly TLR6, CCR2, CD69 and BCL2, while …
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NDE1 in the DISC1 pathway: interactions of schizophrenia-related proteins
… highly similar proteins which are known to play cellular roles including microtubule function and mitosis. Their orthologues have also been shown to be important in neurodevelopment within the mouse brain. To date, most work in the literature has investigated NDEL1, with few focusing on NDE1. In …
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THE ROLE OF P24 PROTEINS IN THE ER TO GOLGI TRANSPORT OF TOLL-LIKE RECEPTORS
… ER-export deficient TMED7 and TMED2 and their cellular localisation observed using confocal microscopy. These data suggest that TMED7 is required for ER-export of TLR4 and TLR2 but not TLR3, while TMED2 is required for the ER-export of TLR2, TLR3 and TLR4. Here, evidence is also presented …
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Structural and functional studies of human ADAM 12 in myoblast fusion and Ebola virus VP40 in assembly
… a monomer in solution consisting of two domains. Cellular localisation studies ofdifferent VP40 mutants showed that the C-terminal domain is required for plasma membrane association. Moreover, we showed that wild type VP40 and an N-terminally truncated form VP40 (31-326) are both released into the …
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Characterisation of the Arabidopsis mid-SUN proteins at the plant NE
… imaging technique used to determine protein localisation at the nuclear periphery was further refined. This was used to show that fluorescent protein fusions of both mid-SUN proteins co-localised with the ER/outer nuclear membrane (ONM) marker mCherry and not the inner nuclear membrane (INM) …
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Using Dictyostelium discoideum to develop flavonoid therapeutics for polycystic kidney disease: the role of MATE transporters
… was dominant earlier in the life cycle, when unicellular D. discoideum cells prey on bacteria. Ddmate2 was also highly transcribed after cells have aggregated and differentiated to form a motile, multicellular slug. DdMmate1 is hypothesised to be a toxin efflux transporter in unicellular and …
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The localisation and regulation of phosphatidylinositol-4-phosphate 5-Kinase gamma splice variants and the discovery of a new mammalian splice variant, PIP5KIγ_v6
… 5-kinases, PIP5Ks) catalyse the majority of cellular synthesis of PI(4,5)P2. To date, three mammalian isoforms (r1, r2, r3) have been found. PIP5KIr is subject to complex C-terminal splice variation, enhancing its transcriptional diversity through evolution and producing at least 5 known …
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Mucosal and systemic immune responses induced by immunisation of cotton rats with recombinant adenoviruses
… from challenge were, however, dependent on the cellular localisation of the foreign gene expressed by the vectors, the replication-capability of the viruses, the route of immunisation and the presence or absence of pre-existing adenovirus-specific immunity in the cotton rat.
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Molekulare Alteration im E-Cadherin-Gen bei malignen Melanomen und kolorektalen Karzinomen
… epithelial tissues and structures in multicellular organisms. Interference with cell attachment, independence of growth control, and increased migration have long been implicated during the neoplastic process. It is now apparent that the hallmarks of epithelial malignancy, that is to say, …
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Epidermal growth factor receptor signalling regulates ommatidial rotation during Drosophila eye development
… a key role in ommatidial rotation, as its sub-cellular localisation is disturbed in argosroulette mutants. Genetic interactions further implicate non-muscle Myosin II as well as genes involved in actin polymerisation/depolymerisation in this process. This study also describes the design of an …
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Elucidating the role of TM9SF2 in membrane trafficking
… mislocalised. One mechanism to prevent enzyme mislocalisation is their capture by vesicles formed by coat protein complex 1 (COP1), via cargo adaptors such as Golgi phosphoprotein 3 (GOLPH3), for recycling to earlier parts of the Golgi. Published genome-wide CRISPR-Cas9 knockout screens …
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The role of Iron Regulated 2 and Iron Regulated Transporter 1 in nickel hyperaccumulation traits in Senecio coronatus
… Ni transport activity and determine their sub-cellular localisation. Full-length ScIREG2 and ScIRT1 protein coding sequences were obtained using RT-PCR and conformed to the predicted sequences derived from the RNA-Seq data. Heterologous expression of ScIRT1 in yeast consistently conferred an …
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SODIUM/IODIDE SYMPORTER-NIS Abundant and Important in Gastric Mucosa
… in gastric mucosa - the focus of this thesis. Cellular localisation of NIS: NIS expression was found basolaterally in gastric mucosal surface cells of several mammals including man, basolaterally in salivary gland ducts and apicolaterally in rat bile ducts. Weak NIS immunoreactivity was found …
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Identification of the Apoptin tumour specific kinase/s
… (HGyV) Apoptin was shown to have a similar cellular localisation to CAV Apoptin, and was also found to be toxic to <br/>transformed cells, though more indiscriminately than CAV Apoptin.<br/>In conclusion, the results presented here in this study propose a novel role for PKG and PKC in the …
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Subcellular labelling of myelin protein (PLP and DM20) in the central nervous system
… provide the best resolution to establish the sub-cellular localisation of the two myelin protein isoforms encoded by the Plp1 gene in mice and thus provide insight into the functions for the two isoforms. The gene is expressed primarily by oligodendroglia of the central nervous system (CNS), with …
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