Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"cellular lipids"”.
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Mass spectrometry methods for imaging cellular lipids, organelles, and proteins
Lipids and their secondary metabolites function as energy storage molecules, structural components in cellular membranes, and messengers that can alter cellular function. Imaging the distributions of various lipid species within tissues and cells can yield insight into the mechanism by which lipids …
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Hormonal regulation of phosphatidylcholine metabolism in Apium graveolens
… accompanied by an increase in the synthesis of cellular phospholipids Metabolic studies involving radiolabelled precursors of phosphatidyicholine, revealed a generalized switching off of the CDP-choline pathway coupled to a concomitant increase in flux through the phospholipid methyltransferase …
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Analysis of Hopanoids From Three Psychrophilic Marine Bacterial Strains
… hopanoids, was also analyzed as a control. Cellular lipids were extracted from freeze-dried cells using a chloroform/methanol/water solvent system. Crude extracts were treated with a solution of periodic acid followed by sodium borohydride reduction in order to convert the complex hopanoids …
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The Effect of Dietary Fats Containing Trans-Octadecenoates on Calcium Transport and Membrane Properties of The Rat Erythrocyte
… the amount of trans fatty acid in the membrane lipids can be correlated to the level of trans acid in the dietary fat. Time studies determined that the equilibrium period for trans-octadecenoate incorporation into cellular lipids is 9 to 12 days. (Ca('2+) + Mg('2+))-ATPase activity using …
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Novel Metabolic Regulation of Invadopodia and Invasion by Acetyl-CoA Carboxylase and Lipogenesis
… that facilitate matrix degradation and cellular invasion. Although cellular lipids have been implicated in several aspects of invadopodia assembly and function, the contribution of de novo fatty acid and phospholipid synthesis has not been elucidated. The data in this dissertation …
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Effect of Fatty Acid Modification on Prostacyclin Production in Cultured Human Endothelial Cells (Arachidonate, Elcosapentaenoate)
… of radiolabelled ('3)H-AA and ('14)C-EPA into cellular lipids was determined after two-dimensional thin-layer chromatography. Neutral lipid contained the vast majority of radiolabel ((TURN)50%). Of the phospholipids, phosphatidylcholine contained the greatest percentage of radiolabel. …
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Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
… study uses the condensing enzyme, EloA, from the cellular slime mold, Dictyostelium discoideum as a model for the family of ELOs. EloA has substrate specificity for monounsaturated and saturated C16 fatty acids and catalyzes the elongation of 16:1Δ9 to 18:1Δ11. Site-directed mutagenesis was used …
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Lipid studies in breath-hold diving mammals and obese, pre-diabetic mice.
Lipidology, or the scientific investigation of cellular lipids in biological systems, as well as their pathways and networks, is becoming an increasingly integral component in the investigation of metabolic disease and, outside the healthcare fields, in the elucidation of adaptations to specific …
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Analytical Potential Of Polymerized Liposomes Bound To Lanthanide Ions For Qualitative And Quantitative Analysis Of Proteins
… often very strong interactions among different cellular components. Such interactions play a variety of organizational, mechanical, and physiological roles at the cellular and organism levels. Antigen-antibody complexes are representative examples of highly selective and potent interactions …
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USE OF MICROALGAE FOR WASTEWATER TREATMENT AND BIOFUEL PRODUCTION: EFFECTS OF ENVIRONMENTAL CONTAMINANTS ON THE CELLULAR RESPONSE
… into biodiesel depends on the accumulation of lipids, mainly triacylglycerols. However, the presence of toxic contaminants in municipal or industrial wastewater could negatively affect the biomass growth and modify the pattern of lipid accumulation in algae cells. This research aims to evaluate …
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Investigating the regulation of tumour associated lipogenesis using the model organism, Saccharomyces cerevisiae
… human disease, where cancer cells accumulate cellular lipid. To investigate the role of the HOG1 pathway in neutral lipid accumulation, a Nile red assay was undertaken to measure lipid accumulation in stationary phase of genomic deletion strains of key HOG pathway proteins. Deletion of the …
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The Use of Spectroscopic Techniques in the Characterization of Mycobacterial Metabolites
… The first is for the cell wall associated lipids, the second is for covalently bound mycolic acids and the third deals with the complex cell wall polysaccharides. Key structural features and major lipid species were readily identifiable using this technique. This approach can be used for …
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A role for myotubularins in vaccinia virus egress
… distinct forms of infectious virus. Intracellular mature virus (IMV) particles are surrounded by a single lipid membrane and are mostly released upon lysis of the infected cell. Some IMVs are wrapped by an additional double membrane to form intracellular enveloped virus (IEV), also called …
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The interaction of hepatitis C virus and intracellular lipid metabolism
… measured incorporation of 14C acetate into total cellular lipids. Toxicity was assayed using mitochondrial enzyme activity assays. Treating genotype 1b replicon cells with 30 μM cerulenin led to inhibition of fatty acid biosynthesis and a corresponding inhibition of HCV RNA replication. However, …