Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"cellular imaging"”.
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Enzyme engineering by directed evolution and applications in cellular imaging and proteomic mapping
… individual protein components within the native cellular context. A wide panoply of enzyme-mediated labeling methodologies have been developed that couple the power of enzyme specificity with the functional diversity of small molecules to specifically tag proteins within the crowded heterogeneous …
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Xanthene/Benzoxanthene Dyes: Novel Synthetic Metholologies, Applications in Molecular Recognition, Cellular Imaging and the Design of a Tunable White (RGB) Light Emitter
<p>My graduate work has focused on the development of new fluorophore materials towards addressing several interdisciplinary research problems. Selective detection of sialic acid via a boron-amide interaction. Concentration changes of sialic acid can be monitored ratiometrically in the visible …
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The synthesis and characterisation of inorganic and organic luminophores suitable for biomolecule conjugation
… which were prepared for applications in cell imaging. A key aim of this thesis was the synthesis, characterisation and identification of novel bioconjugates suitable for applications in cellular imaging. Some preliminary studies of their application in cell imaging are also presented. Chapter …
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New technologies for optical coherence microscopy
… from unacceptable false negative rates. Optical imaging technology that provides real-time, high-resolution imaging of human tissue in vivo with resolution at or near that of histopathology may significantly improve clinicians' capabilities to identify malignancies at curable stages. The ability …
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Fluorinated Nanoparticles: A Novel Technology Platform for Multimodal Biomedical Imaging Applications
… in microbubble contrast agents for ultrasound imaging, positron sources for PET imaging, and as an imaging probe in 19F MRI. Additionally, fluorinated organic compounds generate a high ionization yield of fluorine in secondary ion mass spectrometry (SIMS), which is gaining recognition as an in …
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Silicon based photonic crystal coupled to an optical cavity for highly sensitive biodetection
… to be used in disease diagnosis, DNA sequencing, cellular imaging, flow cytometry, forensics, and monitoring processes in biological systems. In order to achieve better sensitivity and higher signal-to-noise ratio (SNR), metal-enhancement fluorescence and nano-engineered surfaces were used to …
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DNA Walkers: Emerging Analytical Applications, Biomolecular-Nanomaterial Probes and Biomolecule Sensors
… acid sensing, to protein detection and to cellular imaging and analysis. The research described herein focuses on improving the understanding of biophysical processes involved in the design and operation of DNA walkers. Specifically, we developed a series of stochastic DNA walkers capable …
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Advances in Optical Coherence Tomography and Microscopy for endoscopic applications and functional neuroimaging
… Tomography (OCT) is a developing medical imaging technology that generates micron resolution cross-sectional images of subsurface internal tissue structure in situ and in real time, without the need to remove and process specimens. Previous studies have suggested that OCT holds great …
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Fluorescence Off-On Sensors for F-, K+, Fe3+, and Ca2+ Ions
… growth in applying fluorescence technique to cellular imaging. The distinguished merits of fluorescence techniques, such as high sensitivity, non-invasiveness, low cytotoxicity, low cost, and convenience, make it a promising tool to replace radioactive tracers for most biochemical …
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COMPARISON OF E. COLI AND Y. PESTIS LPS TLR4 TIME COURSE ON P388D1 MACROPHAGES USING FLUORESCENT CONJUGATED ANTIBODIES
The innate immune system enables cellular response to infectious agents, and protein interactions are essential for this response. However, the protein interactions involved in cellular response to pathogens are not completely understood. Clarifying the manner in which proteins bind and respond to …
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A New Approach to Drug Delivery: Non- Peptidic, High Load Macrocyclic Alternatives to Cell Penetrating Peptides
… is of interest where a molecule has an intracellular target, for example gene therapy, delivery of cytotoxic agents or cellular imaging. Chapter 2 introduces the mechanisms of cell uptake and the design and applications of cell penetrating peptides. Calixarenes are presented as alternatives …
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CHEMICAL BIOLOGY STRATEGIES FOR THE FORMATION AND CLEAVAGE OF CHEMICAL BONDS
… DNA-protein conjugates which are suitable for cellular applications. Using the method developed, various types of ONs can be easily conjugated to a range of proteins via solvent accessible cysteine residues, including therapeutic full-length IgG antibodies. Moreover, in order to optimise and …
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Upconversion Nanoparticles for pH Sensing and Imaging
… to the micro or nanoscale to provide relevant subcellular information, but the most widely used optical pH sensors (fluorescent dyes, dye-labeled quantum dots) suffer from drawbacks of photobleaching, blinking, and limited tissue penetration. Autofluorescence is also an issue during tissue imaging …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
… fluorescence microscopy is a powerful method for imaging detailed biological structures at the nanoscale. However, imaging capabilities in thick samples such as mammalian cells remain limited due to increased fluorescence background which degrades the achievable localization precision of …
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Surface Modification of Spherical Particles with Bioactive Glycopolymers
… and lectins were extended towards the cellular uptake of fluorescent, magnetic galactose-displaying core-shell nanospheres. These particles were prepared by grafting a galactose-displaying glycocopolymer onto silica-encapsulated iron oxide particles via thiol-ene chemistry. Due to the …
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SYNTHESIS AND CHARACTERIZATIONS OF NANOSTRUCTURED MAGNETIC MATERIALS FOR MRI T1 CONTRAST AGENTS
… and were successfully used for in vitro imaging application. Moreover, the r1 value was 0.616 s-1mM-1, which was significant to offer a brightness in T1 imaging, Hence, it was shown that the nanocomposites combining fluorescent and MR contrast agent were promising candidates for …
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STRUCTURE–ACTIVITY RELATIONSHIP STUDIES OF HYBRID ANTITUMOR AGENTS FOR THE TREATMENT OF NON-SMALL CELL LUNG CANCER
… mass spectrometry (LC–ESMS). A cellular imaging study using confocal fluorescence microscopy was performed to study the subcellular distribution of complex 54. The results show higher cellular levels of 54 than of PT-ACRAMTU and suggest that the drug reaches the nucleus, although …
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Multifunctional Nanoparticles for Cancer Imaging and Therapy
… but also provide contrast for magnetic resonance imaging, a method that can be used to visualize the distribution of the therapeutics. Furthermore, the surface of iron oxide nanoparticles can be readily coated with biocompatible polymers that can provide stability as well as chemical conjugation …
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