Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"cellular identity"”.
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Programming and reprogramming cellular identity
… converge on gene regulatory machinery to shape cellular identity. The work presented here focuses on the mechanisms by which transcription factors, chromatin regulators, miRNAs and signal transduction pathways coordinately regulate two particular medically important gene expression programs: (1) …
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The Functional Cellular Identity and Interconversion of Human White, Beige, and Brown Adipocytes
Since the mid-20th century, the prevalence of obesity has seen an unprecedented growth, with nearly 40 % of the population now qualifying as overweight, and almost 15 % as obese. Due to its systemic nature, obesity represents a significant healthcare concern, and can lead to the development of a …
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FUNCTIONAL STUDY OF MAMMARY EPITHELIAL CELL ARCHITECTURE
Cell organization confers cellular identity and guides cellular functions. Here, the
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Biological and medical implications of discordance in CpG methylation
… expression. It is a critical part of controlling cellular identity and is essential for normal development. DNA methylation is generally studied by comparing methylation levels at individual cytosines or computing region-level averages to identify differential methylation. Here we extended this …
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INTEGRATIVE GENOMICS UNVEILS TRANSCRIPTION FACTOR ROLES IN CELLULAR FATEAND SPATIALLY RESOLVED TRIPLE-NEGATIVE BREAST CANCER IMMUNITY CAPACITY
… investigates transcription factors (TFs) in cellular differentiation, addressing the inefficiency in current cellular conversion strategies due to the complex TF regulatory landscape. We developed a comprehensive screening of 130 developmental TFs, comparing their dosages in a single …
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Molecular targets of chromatin marks H3K4ME3, H3K9ME3 and H3K27ME3 in an adult germinal niche
… in a delicate balance between maintaining cellular identity through self-renewal and differentiating into myriad neural cell types. Understanding exactly how epigenetic mechanisms regulate this balance and the subsequent differentiation process in adult mammalian brain is an ongoing effort. …
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Massively multiplexed imaging probes for comprehensive single-cell analysis
… microscopy techniques are widely used to study cellular physiology in their native tissue environments. In particular, the use of fluorescent probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular …
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Scalable development of multiplexed microparticle technologies for optical single-cell barcoding
… role in developing an understanding of cellular identity and function. However, current imaging techniques, although widely used to distinguish several different cell populations, are not scalable to single cells at a large scale because they rely on fluorescent molecules with broad …
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Regulation of Chromatin Multi-Scale Organization and Function by Linker Histone H1
… for regulating gene expression and maintaining cellular identity. The organizing principles underlying this 3D structure are a subject of much study, and it has proven to be a complex system to unravel. There are multiple scales of genome organization, ranging from single basepair modifications …
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Transposable elements and the regulatory logic of hematopoietic differentiation
… cis-regulatory elements is central to establish cellular identity and function. Understanding this regulatory logic is critical for deriving select cell types in vitro for translational applications. The human hematopoietic system has long been a model system and an important source for adoptive …
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Mechanotransduction at the nuclear envelope: the role of forces in facilitating embryonic stem cell fate decisions
… has focused on the transcriptional regulation of cellular identity, the role of the mechanical properties of cells and the importance of their physical interactions with the local environment remains less well understood. In this project, we explored the impact of cytoskeleton-generated forces …
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Towards causality in gene regulatory network inference
… is key to gaining mechanistic insights into cellular functions, phenotypes, and diseases. Advances in single-cell technologies promise to unveil mechanisms of gene regulation at unprecedented resolution by enabling measurements of genomic and/or epigenetic features for individual cells. …
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Methods for Dissecting High Dimensional Single Cell RNA Sequencing Data
… rapidly advanced into a staple for interrogating cellular identity in heterogeneous populations. Researchers routinely capture transcriptome-wide snapshots of thousands or even millions of individual cells. From these readouts the challenge is to accurately identify distinct patterns of mRNA …
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DISCOVERY OF NOVEL EPIGENETIC MECHANISMS OF CARCINOGENESIS BY GENOME-WIDE PROFILING OF NON-CODING REGULATORY ELEMENTS
… they are under normal circumstances drivers of cellular identity and associated global gene expression programs. We report the discovery of thousands of Variant Enhancer Loci, or VELs, which have gained or lost H3K4me1 at enhancer elements in colon cancer samples. We show that VELs are tightly …
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Generation of induced progenitor-like (iPL) cells using interrupted reprogramming
… the epigenome histone alteration. Importantly, cellular identity and function of parental AEC-II cells were maintained, thereby generating large numbers of younger AEC-II cells. In summary, this novel interrupted reprogramming strategy will allow the production of highly specified functional …
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Targeting Unique Domains of Lsd1 Regulates Pediatric Glioma Innate Immunity and Nk Cell Metabolism
… of chromatin accessibility is a key mechanism of cellular identity, allowing different tissues to develop using the same DNA template. Cancers will often hijack these epigenetic pathways, reactivating developmental genes to drive growth and deactivating tumor suppressor and immune recognition …
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Structure, Function & Dynamics At The Membrane
… biological membrane is necessary for maintaining cellular identity, yet must also allow for interaction with the extracellular environment in order to respond to stimuli. Proteins that are directly embedded in the membrane or that interact more peripherally are responsible for these extracellular …
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Multiscale Modeling Of Cell Fate Switching To Predict Patient-Specific Response To Combination Cancer Therapy
… DNA sequence, but differ in their functional identity, guided by a wide array of regulatory mechanisms controlling cellular lineage commitment and encoded in the unique epigenome of each cell type. Recent experimental studies with induced pluripotent stem cells have allowed researchers to …
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Epigenetic Regulation of Muscle Stem and Progenitor Cells
… importance for resolving and maintaining cellular identity. The mechanisms regulating muscle stem and progenitor cell identity have ramifications for understanding all aspects of myogenesis. The epigenetic mechanisms regulating muscle stem cells are therefore important aspects for …
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An ORC Flip Enables Bidirectional Helicase Loading
… faithfully in every cell cycle to maintain cellular identity and ensure viability. The first step in DNA replication is the licensing of all potential origins by loading two inactive Mcm2-7 replicative DNA helicases around the origin DNA in a head-to-head double hexamer. This conformation …
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